FastQCFastQC Report
Thu 28 Sept 2023
SRR5996796_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5996796_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156607069
Total Bases7.8 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTGA3646744623.285951415130565Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTTTCTTTACTTATTCAATGAAGCGGAGAGATCGGAAGAGCACACGTCT46569972.9736824970525437Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAAGAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTG46050402.940505833743686Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGAGGTGCACAATCGACCGATCCTGAGATCGGAAGAGCACACGTCTGAA35497302.266647363153192Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TAGAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTGA23975251.530917483680127Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGTGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTGA23971361.530669091316689Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTGAA22080701.409942740196485Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATGAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTGA20148661.2865741073284502Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAGAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTGA15221620.9719625108365958Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAAGAGGTGCACAATCGACCGATCCTGAGATCGGAAGAGCACACGTCTGA11037850.704811734903231Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTGA9279890.5925588199342394Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATATAGATGGATACGAATAAGGCGTCCAGATCGGAAGAGCACACGTCTG9209950.5880928657185966Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTTCTTTACTTATTCAATGAAGCGGAGAGATCGGAAGAGCACACGTCTG7058930.45074146684911137Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTTTCTTTACTTATTCAATGAAGCGGAGAGATCGGAAGAGCACACGTCT6849400.4373621218848046Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGATGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTGA5511800.3519509071458326Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTGAA4770120.30459161457137035Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGTTTCTTTACTTATTCAATGAAGCGGAGAGATCGGAAGAGCACACGTCT4472680.2855988576096779Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATATAGATGGATACGAATAAGGCGTCAGATCGGAAGAGCACACGTCTGA4371310.27912596972235015Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAATATAGATGGATACGAATAAGGCGTCAGATCGGAAGAGCACACGTCTG4284590.27358854407779Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAGGTGCACAATCGACCGATCCTGAGATCGGAAGAGCACACGTCTGAAC4010610.2560938037860858Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CAATATAGATGGATACGAATAAGGCGTCCAGATCGGAAGAGCACACGTCT3758340.23998533552786178Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTTCTTTACTTATTCAATGAAGCGGAGAGATCGGAAGAGCACACGTCTG3374920.2155024049393326Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAAGAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTG3158910.2017092855495559Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGAGGTGCACAATCGACCGATCCTGGAGATCGGAAGAGCACACGTCTGA2987390.19075703409020445Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTTTCTTTACTTATTCAATGAAGCGGAGCAGATCGGAAGAGCACACGTC2899120.18512063462473716No Hit
GGTTTCTTTACTTATTCAATGAAGCGGAGAGATCGGAAGAGCACACGTCT2887280.18436460234116253Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGAGGTGCACAATCGACCGATCCTGAGATCGGAAGAGCACACGTCTGAA2746230.17535798463861169Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGTGGTGCACAATCGACCGATCCTGAGATCGGAAGAGCACACGTCTGAA2720440.17371118796687268Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTAGAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTG2581610.16484632631749208Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGAGGTGCACAATCGACCGATCCTGAGATCGGAAGAGCACACGTCTGAA2488110.15887597002406068Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTGTCTTTACTTATTCAATGAAGCGGAGAGATCGGAAGAGCACACGTCT2460260.15709763395163215Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAAGAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTG2149340.1372441240184375Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTATCTTTACTTATTCAATGAAGCGGAGAGATCGGAAGAGCACACGTCT2133930.1362601326763864Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGAGGTGCACAATCGACCGATCCTGCAGATCGGAAGAGCACACGTCTGA2062100.13167349425331495Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAGAGGTGCACAATCGACCGATCCTGAGATCGGAAGAGCACACGTCTGAA1785090.11398527610525677Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATATAGATGGATACGAATAAGGCGTCCAGATCGGAAGAGCACACGTCTGA1714450.10947462403501082Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)