FastQCFastQC Report
Thu 28 Sept 2023
SRR5996798_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5996798_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156527954
Total Bases7.8 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTGA3414253121.81241760816729Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAAGAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTG103641546.621279928056813Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTTTCTTTACTTATTCAATGAAGCGGAGAGATCGGAAGAGCACACGTCT32605942.0830745669875683Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTGA22910041.463638884591822Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGTGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTGA22189351.4175966294173883Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATGAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTGA19340911.235620188327511Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGAGGTGCACAATCGACCGATCCTGAGATCGGAAGAGCACACGTCTGAA18059031.1537255511561852Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTGAA15497260.9900634106544317Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAGAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTGA14642760.9354725226907393Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAAGAGGTGCACAATCGACCGATCCTGAGATCGGAAGAGCACACGTCTGA9343610.5969291593755836Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATATAGATGGATACGAATAAGGCGTCCAGATCGGAAGAGCACACGTCTG9309630.5947583011274779Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTGA9057360.5786416910553881Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAAGAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTG6751850.43135106717104343Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTAGAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTG6043110.38607225390552286Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGATGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTGA5605030.3580849207292392Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTTTCTTTACTTATTCAATGAAGCGGAGAGATCGGAAGAGCACACGTCT5193340.33178354838778507Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTTCTTTACTTATTCAATGAAGCGGAGAGATCGGAAGAGCACACGTCTG4740220.30283536447425874Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAAGAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTG4592810.29341787729493995Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAATATAGATGGATACGAATAAGGCGTCCAGATCGGAAGAGCACACGTCT4571570.29206093117399334Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAATAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTG3539180.22610530001561255Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTGAA3536280.2259200295942027Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CAATATAGATGGATACGAATAAGGCGTCAGATCGGAAGAGCACACGTCTG3197960.20430599891441756Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGTTTCTTTACTTATTCAATGAAGCGGAGAGATCGGAAGAGCACACGTCT3138330.20049645573211797Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGAGAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTG3016320.19270168189894055Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAGGTGCACAATCGACCGATCCTGAGATCGGAAGAGCACACGTCTGAAC2823170.18036203296952313Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAGAGGTGCACAATCGACCGATCCTGATAGATCGGAAGAGCACACGTCTG2666120.17032868135489715Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAAGTGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTG2657600.16978436963406549Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTTTCTTTACTTATTCAATGAAGCGGAGCAGATCGGAAGAGCACACGTC2479210.15838768326327193No Hit
AATATAGATGGATACGAATAAGGCGTCAGATCGGAAGAGCACACGTCTGA2388590.15259830202597552Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTTCTTTACTTATTCAATGAAGCGGAGAGATCGGAAGAGCACACGTCTG2323750.1484559109486603Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGAGGTGCACAATCGACCGATCCTGGAGATCGGAAGAGCACACGTCTGA2110010.13480084202723303Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGTTTCTTTACTTATTCAATGAAGCGGAGAGATCGGAAGAGCACACGTCT2030720.1297352931604792Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTGTCTTTACTTATTCAATGAAGCGGAGAGATCGGAAGAGCACACGTCT1795120.11468366857973497Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCCACGTTCTAGCATTCAAGGTCCCAAGATCGGAAGAGCACACGTCTG1584110.10120300939984177Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTATCTTTACTTATTCAATGAAGCGGAGAGATCGGAAGAGCACACGTCT1567950.10017060594812348Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)