FastQCFastQC Report
Thu 28 Sept 2023
SRR5996800_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5996800_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140794709
Total Bases7 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTGA82662625.8711453425426665Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGCTCGAATATATTAGCATGGAATAATAGAATAGATCGGAAGAGCACAC45989813.2664444798135137No Hit
CAAGAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTG27603751.9605672823969542Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGCTCGAATATATTAGCATGGAATAATAGAATAGATCGGAAGAGCACACG11509990.8175016008591629No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATAAGATCGGAAGAGCACA11354120.806430872341943No Hit
GTGCTCGAATATATTAGCATGGAATAATAGAATAGATCGGAAGAGCACAC10967880.7789980232850937No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGAGATCGGAAGAGCACACGTCT10371180.7366171693284298Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAGATCGGAAGAGCACAC9715840.6900713861342617No Hit
CTTTGATGATTCATAATAACTTTTCGAATCGCATAGATCGGAAGAGCACA9293410.6600681279862584No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATAGATCGGAAGAGCACAC8387280.5957098856605471No Hit
GCTCGAATATATTAGCATGGAATAATAGAATAGATCGGAAGAGCACACGT7579840.5383611396931116No Hit
ATGCTCGAATATATTAGCATGGAATAATAGAATAGATCGGAAGAGCACAC6608220.46935144416541963No Hit
TGGCTCGAATATATTAGCATGGAATAATAGAATAGATCGGAAGAGCACAC5704340.4051530089813247No Hit
TAGAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTGA5581040.39639557762074706Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGTGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTGA5522160.3922136022881371Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATGAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTGA4711670.33464822886206613Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTCGAATATATTAGCATGGAATAATAGAATAGATCGGAAGAGCACACGT4095640.29089445399542674No Hit
GAGAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTGA3559520.2528163185450385Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGCTCGAATATATTAGCATGGAATAATAGAAAGATCGGAAGAGCACACG3294920.23402299869095222No Hit
AAGAGGTGCACAATCGACCGATCCTGAGATCGGAAGAGCACACGTCTGAA3125360.22197993249874182Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCGATATAGTGTAACGGCTATCACATCACGAGATCGGAAGAGCACACGT2934020.20838993317568488No Hit
GGCTCGAATATATTAGCATGGAATAATAGAATAGATCGGAAGAGCACACG2876070.2042740114616097No Hit
AGAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTGAA2854430.2027370218862415Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TAGCTCGAATATATTAGCATGGAATAATAGAATAGATCGGAAGAGCACAC2853550.20267451953752041No Hit
TGCTCGAATATATTAGCATGGAATAATAGAATAAGATCGGAAGAGCACAC2791040.198234722016436No Hit
GTGCTCGAATATATTAGCATGGAATAATAGAATAAGATCGGAAGAGCACA2693930.19133744578427306No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGCAGATCGGAAGAGCACACGTC2429480.17255477973962785No Hit
CTGCTCGAATATATTAGCATGGAATAATAGAATAGATCGGAAGAGCACAC2240910.15916152076424975No Hit
CAAGAGGTGCACAATCGACCGATCCTGAGATCGGAAGAGCACACGTCTGA2188430.15543410796779303Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGTTCGAATATATTAGCATGGAATAATAGAATAGATCGGAAGAGCACAC2175250.1544979932449024No Hit
AGGAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTGA2168690.15403206664534533Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCAGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGTC2130140.1512940376189847No Hit
GGGCTCGAATATATTAGCATGGAATAATAGAATAGATCGGAAGAGCACAC2019380.14342726472768233No Hit
GTTGATGATTCATAATAACTTTTCGAATCGCATAGATCGGAAGAGCACAC2016730.1432390474275564No Hit
TTCTCGAATATATTAGCATGGAATAATAGAATAGATCGGAAGAGCACACG2000260.1420692591509245No Hit
TAAGAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTG1999650.14202593365919738Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTCGAATATATTAGCATGGAATAATAGAATAAGATCGGAAGAGCACACG1855520.13178904329423344No Hit
ATTAAGCCATGCATGTCTAAGTATAAGCAAAGATCGGAAGAGCACACGTC1688520.11992780211648435No Hit
AGCTCGAATATATTAGCATGGAATAATAGAATAGATCGGAAGAGCACACG1639670.11645821150850207No Hit
ATGCTCGAATATATTAGCATGGAATAATAGAATAAGATCGGAAGAGCACA1622470.11523657469259017No Hit
ATTTTCTTTACTTATTCAATGAAGCGGAGAGATCGGAAGAGCACACGTCT1575280.11188488624242264Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTAGAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTG1572620.11169595868833396Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATATAGATGGATACGAATAAGGCGTCCAGATCGGAAGAGCACACGTCTG1543910.10965681956130893Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGCTCGAATATATTAGCATGGAATAATAGATCGGAAGAGCACACGTCTG1504010.10682290624997846Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGATATAGTGTAACGGCTATCACATCACGAGATCGGAAGAGCACACGTC1496740.10630655161906688No Hit
AAGATGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTGA1443090.10249603910896964Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)