FastQCFastQC Report
Thu 28 Sept 2023
SRR5996802_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5996802_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133914492
Total Bases6.6 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTGA1489481611.12263189558304Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAAGAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTG1486275611.098691245455345Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAGAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTGA9765170.7292093524874066Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAAGAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTG9180550.6855531363999051Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGTGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTGA8726460.6516441850072507Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTAGAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTG8416760.6285174871140907Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTTTCTTTACTTATTCAATGAAGCGGAGAGATCGGAAGAGCACACGTCT8308240.6204138085368684Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTGA7854820.5865548890705571Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAAGAGGTGCACAATCGACCGATCCTGAGATCGGAAGAGCACACGTCTGA7363290.549850123764051Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGAGGTGCACAATCGACCGATCCTGAGATCGGAAGAGCACACGTCTGAA7242670.5408428835319781Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CAATAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTG6349420.47413987128443125Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTGA6233250.465464932652696Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAAGAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTG6042170.4511961259577492Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGAGAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTG4186070.3125927550843414Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTGA3660420.2733400952601904Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAAGTGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTG3362380.2510841022344318Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATATAGATGGATACGAATAAGGCGTCCAGATCGGAAGAGCACACGTCTG3358470.25079212487323627Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGATGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTGA3079090.22992955833338785Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTGAA3050070.22776250385208494Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CAATATAGATGGATACGAATAAGGCGTCCAGATCGGAAGAGCACACGTCT2593070.19363624961516487Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGGCCTTTCCTTCTGGCTAACCTTGAGTAGATCGGAAGAGCACACGTC2550800.1904797577845421No Hit
AAGAGGTGCACAATCGACCGATCCTGATAGATCGGAAGAGCACACGTCTG2538560.1895657416973213Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTCCACGTTCTAGCATTCAAGGTCCCAAGATCGGAAGAGCACACGTCTG2223530.1660410286289254Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCAACGGGGCCTTTCCTTCTGGCTAACCTAGATCGGAAGAGCACACGTCT2162070.16145153281841967Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAAGAGGTGCACAATCGACCGATCCTGAAGATCGGGAGAGCACACGTCTG1860160.13890654941214278Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CAATATAGATGGATACGAATAAGGCGTCAGATCGGAAGAGCACACGTCTG1544460.1153318044174039Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATACTGAATATCTAAATATTATTATTACTTAGATCGGAAGAGCACACGT1413080.10552106638316634No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTAGATCGGAAGAGCACACGTC1385970.1034966402291994No Hit
ATTTTCTTTACTTATTCAATGAAGCGGAGAGATCGGAAGAGCACACGTCT1343280.10030878510146608Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTACATGGTATAACTGTGGTAATTCTAGAAGATCGGAAGAGCACACGTCT1342310.10023635082004419Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)