FastQCFastQC Report
Tue 4 Jul 2023
SRR6001742_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6001742_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5968378
Total Bases168.3 Mbp
Sequences flagged as poor quality0
Sequence length24-38
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCTGAGGCGTGATGACGAGGCAC1451642.4322186027761648No Hit
ACGTAGCTCAGTTGGTTAGAGCACATC492050.8244283455236917No Hit
AAGGCTGAGGCGTGATGACGAGGC421120.7055853365855849No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTG395460.6625920811315905No Hit
ACGTAGCGCAGCCTGGTAGCGCACCG346650.5808110679316894No Hit
TGGGGCTGATTCTGGATTCGACGGGATTTGCG344800.5777113983062064No Hit
AGGGACGGCATCACAATCTAGTGGTCA270710.453573818548356No Hit
ATCTCCGGGGGTAGAGCACTGTTTCGGC270040.4524512354948028No Hit
ACTGGTTCGAGTCCAGTCAGAGGAGCCA226350.3792487674205622No Hit
TAAGGCTGAGGCGTGATGACGAGGCAC218490.36607936025499727No Hit
TATTCATCTCCGGGGGTAGAGCACT212650.35629445722104064No Hit
TGCCGACTTAGCTCAGTAGGTAGAGCAACTG205930.3450351167436111No Hit
AAGGCTGAGGCGTGATGACGAGGCA190920.3198859053498287No Hit
AAAATGCCCTGCTTCCAGGAAAAGC177410.29724993959832974No Hit
ACTTGCCCCGAGATGAGTTCTCCCT172950.28977722255527383No Hit
TAGGCTGAGGCGTGATGACGAGGCAC172870.28964318278768536No Hit
TAGGGACGGCATCACAATCTAGTGGTCA171500.2873477517677332No Hit
TAAGCTCTTGATCGAAGCCCCGGT168570.28243854527980633No Hit
AAAGGCTGAGGCGTGATGACGAGGCAC161600.27076033052866294No Hit
AGGCACGCTGATATGTAGGTGAAGCGAC159430.26712450183282627No Hit
TTAGGGACGGCATCACAATCTAGTGGTCA158410.26541549479607357No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTC151240.253402180625959No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCC142990.23957932959340042No Hit
AAGGCTGAGGCGTGATGACGAGGCCC138390.2318720429570647No Hit
TCCCCATGCGAGAGTAGGGAACTGCC136930.22942581719857555No Hit
TGAGCTCGGATATACTTAGAAGTTCGCCG133650.22393018672744922No Hit
GGGGGCTGATTCTGGATTCGACGGGATTTGCG131220.21985872878695015No Hit
TAGCGAAGCTCTTGATCGAAGCCCCGGT119900.20089210167318494No Hit
TTCCCCATGCGAGAGTAGGGAACTGCC118540.1986134256241813No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGC118060.1978091870186506No Hit
ATAGCTCAGTTGGTAGAGCAGCGC114260.1914422980581994No Hit
GCCGATATAGCTCAGTTGGTAGAGCAGCGCA109610.18365123656712093No Hit
TGGGGCTGATTCTGGATTCGACGG108040.18102070612819765No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGA101970.17085043876242423No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGG101250.16964408085412822No Hit
TCGACTTAGCTCAGTAGGTAGAGCAACTG100530.16843772294583217No Hit
ACAGCTCAGTTGGTAGAGCAGCGC99370.1664941463157997No Hit
AAAATGCCCTGCTTCCAGGAAAAGCCTCT98510.16505321881422388No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCG97790.16384686090592787No Hit
ATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCA96850.16227189363676361No Hit
ACCGGAGTGGACGCATCACTGGTGTTCGGGT96600.16185301936304972No Hit
AGAACGTCGTGAGACAGTTCGGTCCCT95840.1605796415709595No Hit
ATCTCCGGGGGTAGAGCACTGTTTCCGC95370.15979215793637735No Hit
AAACCGGGAGATAGCTGGTTCTCCCCGAAAGCT93810.15717838246840263No Hit
ATGAAAGCTACTAAACTGGTACTGGGCG89970.1507444736241572No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGA89560.15005751981526638No Hit
ATAGCTCAGTTGGTAGAGCAGCGCA89560.15005751981526638No Hit
TAGGCGTGTGACTGCGTACCTTTTGT88850.14886791687791892No Hit
ATCTCCGGGGGTAGAGCACTGTTTC88040.14751076423108592No Hit
GCCGACTCAGCTCAGTAGGTAGAGCAACTG86860.14553367765915629No Hit
AAAACCGGGAGATAGCTGGTTCTCCCCGAAAG84710.1419313589052168No Hit
TTTGCCCCGAGATGAGTTCTCCCT81690.13687135767875291No Hit
TAGGCACGCTGATATGTAGGTGAAGCGAC81530.136603278143576No Hit
AAATGGTTCAACGCTGACAAAGGCTTCGGCT79510.13321877401196774No Hit
ACGTAGCTCAGTTGGTTAGAGCAC78650.13177784651039193No Hit
ACTAACTGGAGGACCGAACCGACT75570.12661731545823673No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTT72640.12170810897030986No Hit
AAATGGTTCAACGCTGACAAAGGCTTCGGC72580.1216075791446185No Hit
GCCGATATAGCTCAGTTGGTAGAGCAGCGC72130.12085360545193351No Hit
TATGGTTCAACGCTGACAAAGGCTTCGGCT71670.12008287678829993No Hit
ATTGAGCAGGGGCAACGTGGCCCCGCGGC71630.12001585690450571No Hit
ACCGCCAGCTAAGGTCCCAAAGTCATGG71030.11901055864759237No Hit
ACAGCTCAGTCGGTAGAGCAGGGGAT70940.11885976390905535No Hit
AGTTCAGTCGGTAGAACGGCGGGC69360.11621247849918352No Hit
ACCGCCATCCAGAGCAATGAATTCCGC68870.11539148492270429No Hit
ATGTCCGGTAAAATGACTGGTATCGT68510.11478830596855628No Hit
AGACCGCCAGCTAAGGTCCCAAAGTC68360.11453698140432794No Hit
AACTTGCCCCGAGATGAGTTCTCCCT68100.11410135215966549No Hit
TATGGTTCAACGCTGACAAAGGCTTCGGC67570.11321333869939203No Hit
ACAGCTCAGTTGGTAGAGCAGCGCA67130.11247611997765557No Hit
AGAACGATGGTTACAAATCTCTGGACG66580.11155459657548498No Hit
AGCTCAGTCGGTAGAGCAGGGGAT65620.10994611936442364No Hit
TTTTCGGACGCGGGTTCAACTCCCGCC62860.10532174738262222No Hit
AAGTCAGATGTGAAATCCCCGGGCTC62650.10496989299270253No Hit
AAAATGGTTCAACGCTGACAAAGGCTTCGGCT62240.10428293918381173No Hit
TCCCCATGCGAGAGTAGGGAACTGCCAGGCAT62090.10403161461958342No Hit
ACTTTCAGCGGGGAGGAAGGGAGT61710.10339492572353828No Hit
GCCGATACAGCTCAGTTGGTAGAGCAGCGCA61040.10227234266998506No Hit
TTTGAGCAGGGGCAACGTGGCCCCGCGGC60990.10218856781524226No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)