FastQCFastQC Report
Tue 4 Jul 2023
SRR6001743_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6001743_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6838687
Total Bases194.5 Mbp
Sequences flagged as poor quality0
Sequence length24-38
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCTGAGGCGTGATGACGAGGCAC748211.094084288402145No Hit
AAAATGCCCTGCTTCCAGGAAAAGCCTCT491440.7186174773022951No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCC401150.5865892093028969No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTG364780.5334064857771674No Hit
ACGTAGCTCAGTTGGTTAGAGCACATC302000.4416052379645391No Hit
ACGTAGCGCAGCCTGGTAGCGCACCG295910.43270001975525424No Hit
ATAGCTCAGTTGGTAGAGCAGCGC291510.4262660361557708No Hit
AGGGACGGCATCACAATCTAGTGGTCA252050.36856490142040427No Hit
TAGGGACGGCATCACAATCTAGTGGTCA232190.3395242390827362No Hit
TTAGGGACGGCATCACAATCTAGTGGTCA223770.3272119341037249No Hit
ACTTGCCCCGAGATGAGTTCTCCCT222640.32555957013385756No Hit
TGAGCTCGGATATACTTAGAAGTTCGCCG214690.31393453158479107No Hit
ACCGGAGTGGACGCATCACTGGTGTTCGGGT203030.29688447504616017No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGA192480.28145753709739896No Hit
TAGCGAAGCTCTTGATCGAAGCCCCGGT176190.2576371750893117No Hit
ACTGGTTCGAGTCCAGTCAGAGGAGCCA168710.24669940297019002No Hit
TGGGGCTGATTCTGGATTCGACGGGATTTGCG164970.2412305169106292No Hit
AAAATGCCCTGCTTCCAGGAAAAGC161940.236799841840985No Hit
AAGGCTGAGGCGTGATGACGAGGC160000.23396303998121276No Hit
ATAGCTCAGTTGGTAGAGCAGCGCA159000.23250077098133018No Hit
TGCCGACTTAGCTCAGTAGGTAGAGCAACTG158090.23117010619143705No Hit
ACAGCTCAGTTGGTAGAGCAGCGC144610.21145872007301988No Hit
GCCGATATAGCTCAGTTGGTAGAGCAGCGCA132760.1941308324244113No Hit
AGGCACGCTGATATGTAGGTGAAGCGAC132750.19411620973441246No Hit
ATGAAAGCTACTAAACTGGTACTGGGCG130730.19116242635464964No Hit
AGCTCAGTAGGTAGAGCAACTGAC130580.19094308600466728No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCCT124090.18145296019542934No Hit
ATCTCCGGGGGTAGAGCACTGTTTCGGC123870.18113126101545518No Hit
AGACCGCCAGCTAAGGTCCCAAAGTC113680.16623073990665166No Hit
TAAGGCTGAGGCGTGATGACGAGGCAC108610.15881703607724698No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACTCC105530.15431324755760867No Hit
GCGGGAATAGCTCAGTTGGTAGAGCACGACC96900.14169386608862197No Hit
ATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCA94060.13754102212895544No Hit
AGGTTAATGAGGCGAACCGGGGGAACTGA93630.13691224645900596No Hit
ACCGCCAGCTAAGGTCCCAAAGTCATGG93130.13618111195906465No Hit
ACTAACTGGAGGACCGAACCGACT90150.13182355033941456No Hit
TGGGGCTGATTCTGGATTCGACGG88480.12938156110961066No Hit
ACAGCTCAGTTGGTAGAGCAGCGCA88410.1292792022796189No Hit
AACTTGCCCCGAGATGAGTTCTCCCTGACCC87900.1285334450896788No Hit
AAAGGCTGAGGCGTGATGACGAGGCAC86990.12720278029978563No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACC86600.12663249538983143No Hit
GCCGACTCAGCTCAGTAGGTAGAGCAACTGA82810.12109049588027643No Hit
AACTTGCCCCGAGATGAGTTCTCCCT82670.12088577822029288No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGC82450.12056407904031871No Hit
TCCCCATGCGAGAGTAGGGAACTGCC80360.11750793683056411No Hit
TAGGCGTGTGACTGCGTACCTTTTGT80360.11750793683056411No Hit
AGCTCAGTCGGTAGAGCAGGGGAT79280.11592868631069093No Hit
AGGGCTTACGGGTTCAAGTCCCGTCCTCGGT77920.11394000047085062No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGG74640.10914375815123575No Hit
CGGAGGTTCGAATCCTCCCGGATGC74000.10820790599131092No Hit
TCCCCATGCGAGAGTAGGGAACTGCCAGGCAT72860.10654091933144476No Hit
AGCTCAGGTGGTTAGAGCGCACCCC72480.10598525711148939No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGC72380.10583903021150112No Hit
TTTCCAAAGGAATTCGCTCCACTGTG71690.10483006460158215No Hit
AGGTAACATCAAATCGTACCCCAAACCG70650.10330930484170427No Hit
TAGGCTGAGGCGTGATGACGAGGCAC68760.10054561643192618No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)