FastQCFastQC Report
Fri 7 Jul 2023
SRR6001744.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6001744.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38289935
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCTGTCTGTGACGCCACTGTAGGCACCATCAATTCGTATGCCGTCTTC11212432.9282969532332714No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA3586050.9365516029212376Illumina Single End Adapter 1 (95% over 22bp)
ATCCTGCTGGTGTCGCCACTGTAGGCACCATCAATTCGTATGCCGTCTTC1631890.42619294078195746No Hit
ATCCGGGTAAGACCTCCACTGTAGGCACCATCAATTCGTATGCCGTCTTC1473580.3848478719015846No Hit
ATCCGGGTGGGACCTCCACTGTAGGCACCATCAATTCGTATGCCGTCTTC1452800.37942085824904115No Hit
ATCCGGGTAGGACCTCCACTGTAGGCACCATCAATTCGTATGCCGTCTTC1376680.359540960307193No Hit
TCCTGGGCGAAATCACCACTGTAGGCACCATCAATTCGTATGCCGTCTTC1346770.3517295080286765No Hit
CCCTCACTGGGGTCGCCACTGTAGGCACCATCAATTCGTATGCCGTCTTC1232890.3219880106874039No Hit
TTCCCAGCTCGCCCCCCACTGTAGGCACCATCAATTCGTATGCCGTCTTC1083380.2829411959043545No Hit
TGCTAAGGCATTGGGTTCTGTAGGCACCATCAATTCGTATGCCGTCTTCT1000430.26127753938469733No Hit
ATCCGATTGGAAGCACCACTGTAGGCACCATCAATTCGTATGCCGTCTTC961920.25122006605652375No Hit
CCCCCCATCGTGAGTGCCACTGTAGGCACCATCAATTCGTATGCCGTCTT903290.235907948133106No Hit
ACCTCGCATCCTTCACCACTGTAGGCACCATCAATTCGTATGCCGTCTTC817940.2136174950414515No Hit
TGCTAAGGCATTGGGTTTTACCCTGTAGGCACCATCAATTCGTATGCCGT790970.20657386856363166No Hit
ATCCCTTAGCTCTCACCACTGTAGGCACCATCAATTCGTATGCCGTCTTC782990.20448976996174062No Hit
TGCTAAGGCATTGGGTTTTACCTGTAGGCACCATCAATTCGTATGCCGTC716470.1871170583078817No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATTCG681440.17796844000910422No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAT673370.17586083653576323Illumina Single End Adapter 1 (95% over 22bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTAGGCACCATCAATTC653570.1706897648167854No Hit
AATTCCCCGTCGCGGAGCCACTGTAGGCACCATCAATTCGTATGCCGTCT644580.1683418893241788No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATTCG621200.16223584605197164No Hit
ACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGGCACCATC612820.16004728135474766No Hit
TGCTAAGGCATTGGGTTTCTGTAGGCACCATCAATTCGTATGCCGTCTTC611210.1596268053210328No Hit
AAAGATTGGAAATCTTTTGGGCCTGTAGGCACCATCAATTCGTATGCCGT581410.1518440812187328No Hit
AGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATTCGTA578400.15105797385135286No Hit
ACCTCCCCTGGAGCACCACTGTAGGCACCATCAATTCGTATGCCGTCTTC567030.14808852509151554No Hit
TCCTGAGTGCGAGCTCCACTGTAGGCACCATCAATTCGTATGCCGTCTTC560690.14643273748048932No Hit
TCCCGCTAGAGACCACCACTGTAGGCACCATCAATTCGTATGCCGTCTTC558430.1458425040418585No Hit
TGCTAAGGCATTGGGTTTTCTGTAGGCACCATCAATTCGTATGCCGTCTT536170.14002896583658342No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC535820.13993755800316715Illumina Single End Adapter 1 (95% over 22bp)
TTCCTGTCCGTTTCACCACTGTAGGCACCATCAATTCGTATGCCGTCTTC499710.1305068812469909No Hit
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTATG494820.12922978323154635No Hit
CGTCCGATCAACTGTAGTCTGTAGGCACCATCAATTCGTATGCCGTCTTC487930.1274303547394374No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATTCGTA481230.12568054764261158No Hit
AGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCT480500.12548989701862903No Hit
GCCGATATAGCTCAGTTGGTAGAGCAGCGCACTGTAGGCACCATCAATTC479260.12516605212309712No Hit
AAAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTCTGTAGGCACCATCAA476410.12442173119385029No Hit
TGCTAAGGCATTGGGTTTTACTGTAGGCACCATCAATTCGTATGCCGTCT464260.12124857354811389No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG456560.11923760121295585Illumina Single End Adapter 1 (95% over 22bp)
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATA433150.11312372298359868Illumina Single End Adapter 1 (95% over 22bp)
CGGCACGTAGCGCAGCCTGGTAGCGCACCGCTGTAGGCACCATCAATTCG421000.1099505653378623No Hit
ATACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTAT419630.10959276896134716No Hit
AAATCCTGCTGGTGTCGCCACTGTAGGCACCATCAATTCGTATGCCGTCT411470.10746166061655629No Hit
AGAAAGCACCGTTTCCCGTCCGATCAACCTGTAGGCACCATCAATTCGTA410770.10727884494972373No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGACTGTAGGCACCATCAATTC409090.10684008734932562No Hit
CGTCCGATCAACTGTAGTTCTGTAGGCACCATCAATTCGTATGCCGTCTT407640.10646139775374391No Hit
ATGCCTTGCTAAGGCATTGGGTTCTGTAGGCACCATCAATTCGTATGCCG407250.10635954331079435No Hit
AAAGATTGGAAATCTTTTGGGCTCTGTAGGCACCATCAATTCGTATGCCG407200.10634648504887774No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACCTGTAGGCACCATCAATT402250.10505371711913326No Hit
ATGCCTTGCTAAGGCATTGGGTTTTACCCTGTAGGCACCATCAATTCGTA398550.10408740573730407No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)