FastQCFastQC Report
Tue 4 Jul 2023
SRR6001745.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6001745.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11745422
Total Bases587.2 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA2226291.8954533945225636Illumina Single End Adapter 1 (95% over 22bp)
ACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGGCACCATC925380.7878644121939594No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATTCG635640.5411810661209108No Hit
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTATG606110.5160393555889264No Hit
AGGGCTTACGGGTTCAAGTCCCGTCCTCGGTACCACTGTAGGCACCATCA538070.4581104025040565No Hit
CGGCACGTAGCGCAGCCTGGTAGCGCACCGCTGTAGGCACCATCAATTCG433970.3694801259588629No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAT421100.35852266525630155Illumina Single End Adapter 1 (95% over 22bp)
TCTTCGAGTGTGACGGTTACTTGAACAACTGTAGGCACCATCAATTCGTA389120.3312950356317551No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATTCG386670.3292091165391929No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC324770.27650773211894814Illumina Single End Adapter 1 (95% over 22bp)
TACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGGCACCAT314290.26758510677606984No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT301770.25692563451530304No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGCCTGTAGGCACCATCAATT299830.255273927152213No Hit
GCGGGAGTGGCGAAATTGGTAGACGCACCAGCTGTAGGCACCATCAATTC292770.24926307458344193No Hit
AGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATTCGTA286350.24379711516538102No Hit
GCCGAGGTGGTGGAATTGGTAGACACGCTACCCTGTAGGCACCATCAATT279350.23783734632948908No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG276590.23548749461705162Illumina Single End Adapter 1 (95% over 22bp)
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATA271070.23078779119217685Illumina Single End Adapter 1 (95% over 22bp)
AAAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTCTGTAGGCACCATCAA268040.22820806268178362No Hit
TGCCGACTTAGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATTC262710.22367012441102582No Hit
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA251090.21377690814344516Illumina Single End Adapter 1 (95% over 22bp)
GATCGTCGGACTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAA247060.21034578408506732Illumina Single End Adapter 1 (95% over 22bp)
ATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCACTGTAGGCACCATCAAT238890.20338988245803344No Hit
TCAAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTCTGTAGGCACCATCA213130.18145793314195097No Hit
AGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATTCGTATGCCGT204700.17428066867244105No Hit
ATCGTCGGACTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAA187110.15930462098339251Illumina Single End Adapter 1 (95% over 22bp)
CGCTGTGCGGGTTCAAGTCCCGCTCCGGGTACCACTGTAGGCACCATCAA184070.15671637851751943No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGACTGTAGGCACCATCAATTC174180.14829607654795204No Hit
TAGGGACGGCATCACAATCTAGTGGTCACTGTAGGCACCATCAATTCGTA173960.14810876952739543No Hit
AGCGCACGCAGGCGGTTTGTTAAGTCAGATGCTGTAGGCACCATCAATTC168310.14329838468128264No Hit
AGACCGCCAGCTAAGGTCCCAAAGTCATGGCTGTAGGCACCATCAATTCG164990.14047175146197388No Hit
ACCGGAGTGGACGCATCACTGGTGTTCGGGTCTGTAGGCACCATCAATTC163380.1391010046297187No Hit
AGGTAACATCAAATCGTACCCCAAACCGCTGTAGGCACCATCAATTCGTA155320.1322387565129631No Hit
TGTCACTGGTTCGAGTCCAGTCAGAGGAGCCACTGTAGGCACCATCAATT152380.12973565360188846No Hit
AGGTTAATGAGGCGAACCGGGGGAACTGACTGTAGGCACCATCAATTCGT149920.12764122055384644No Hit
TCCCCATGCGAGAGTAGGGAACTGCCAGGCATCTGTAGGCACCATCAATT148290.12625344581063158No Hit
TTAGGGACGGCATCACAATCTAGTGGTCACTGTAGGCACCATCAATTCGT144260.12282232175225379No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATC138870.11823329974861696Illumina Single End Adapter 1 (95% over 22bp)
ACCGCCAGCTAAGGTCCCAAAGTCATGGCTGTAGGCACCATCAATTCGTA133020.11325263579290723No Hit
ACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCCTGTAGGCACCATCAATT131490.11195000060449084No Hit
GCCGATATAGCTCAGTTGGTAGAGCAGCGCACTGTAGGCACCATCAATTC130290.11092832594690935No Hit
TAGGGCTTACGGGTTCAAGTCCCGTCCTCGGTACCACTGTAGGCACCATC128620.10950649538177513No Hit
AAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTCTGTAGGCACCATCAAT127400.10846779281323395No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACA124380.10589657825832055Illumina Single End Adapter 1 (95% over 22bp)
TTGAGGCGAACCGGGGGAACTGACTGTAGGCACCATCAATTCGTATGCCG124370.10588806430284071No Hit
AGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCCTGTAGGCACCATCA120530.10261870539857998No Hit
TAGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATTCGT119850.10203975642595048No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)