FastQCFastQC Report
Tue 4 Jul 2023
SRR6001746.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6001746.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16428973
Total Bases821.4 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA4701602.8617735265618856Illumina Single End Adapter 1 (95% over 22bp)
GATCGTCGGACTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAA1170600.7125217139257579Illumina Single End Adapter 1 (95% over 22bp)
ATCGTCGGACTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAA1108940.6749904574071672Illumina Single End Adapter 1 (95% over 22bp)
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGCCTGTAGGCACCATCAATT945960.5757876648771655No Hit
ACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGGCACCATC940750.5726164380451535No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAT902480.5493222248280523Illumina Single End Adapter 1 (95% over 22bp)
GCCGACTTAGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATTCG892730.5433875872825404No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTAGGCACCATCAATTC873350.5315913538843846No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACCTGTAGGCACCATCAATT831590.506172844766377No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATTCG820560.499459095830275No Hit
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA746460.45435585048438515Illumina Single End Adapter 1 (95% over 22bp)
GCCGACTTAGCTCAGTAGGTAGAGCAACTGACTGTAGGCACCATCAATTC737770.4490664145592059No Hit
GCCGATATAGCTCAGTTGGTAGAGCAGCGCACTGTAGGCACCATCAATTC734860.44729515350716076No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC714140.4346832878719808Illumina Single End Adapter 1 (95% over 22bp)
AGGGCTTACGGGTTCAAGTCCCGTCCTCGGTACCACTGTAGGCACCATCA702910.427847802781099No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG613000.3731213143998715Illumina Single End Adapter 1 (95% over 22bp)
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTATG606790.3693414067939609No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATA584360.3556886970354142Illumina Single End Adapter 1 (95% over 22bp)
GCCGAGGTGGTGGAATTGGTAGACACGCTACCCTGTAGGCACCATCAATT556720.3388647604448556No Hit
GGCTACGTAGCTCAGTTGGTTAGAGCACATCCTGTAGGCACCATCAATTC552350.33620482546291847No Hit
GCGGGAGTGGCGAAATTGGTAGACGCACCAGCTGTAGGCACCATCAATTC494770.3011569865018343No Hit
AAAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTCTGTAGGCACCATCAA467280.28442435202736044No Hit
TCGTCGGACTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAA457260.278325370672896Illumina Single End Adapter 1 (95% over 22bp)
AGGTAACATCAAATCGTACCCCAAACCGCTGTAGGCACCATCAATTCGTA435400.26501960895547155No Hit
AGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATTCGTA433630.26394224398567095No Hit
AGACCGCCAGCTAAGGTCCCAAAGTCATGGCTGTAGGCACCATCAATTCG426740.25974843345350923No Hit
CGGCACGTAGCGCAGCCTGGTAGCGCACCGCTGTAGGCACCATCAATTCG413540.2517138472380471No Hit
TCTTCGAGTGTGACGGTTACTTGAACAACTGTAGGCACCATCAATTCGTA398280.2424253786283537No Hit
TCAAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTCTGTAGGCACCATCA385250.23449426814445432No Hit
ACCGGAGTGGACGCATCACTGGTGTTCGGGTCTGTAGGCACCATCAATTC372030.22644750831351418No Hit
AGCTCAGTAGGTAGAGCAACTGACTGTAGGCACCATCAATTCGTATGCCG370720.22565013649970697No Hit
AGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATTCGTATGCCGT349540.21275827770853356No Hit
TGCCGACTTAGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATTC334230.20343937506014528No Hit
AGGTTAATGAGGCGAACCGGGGGAACTGACTGTAGGCACCATCAATTCGT331130.20155246466105947No Hit
ACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA328790.20012815165013662Illumina Single End Adapter 1 (95% over 22bp)
ACCGCCAGCTAAGGTCCCAAAGTCATGGCTGTAGGCACCATCAATTCGTA326290.19860644971539002No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATC303460.18471026764728388Illumina Single End Adapter 1 (95% over 22bp)
GCCGACTTAGCTCAGTAGGTAGAGCAACTGACCTGTAGGCACCATCAATT299590.18235467305229608No Hit
AGAACTCTGAACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTG296370.18039472096034245Illumina Single End Adapter 1 (95% over 22bp)
TTGAGGCGAACCGGGGGAACTGACTGTAGGCACCATCAATTCGTATGCCG281650.17143493996855433No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACA272300.16574377473260196Illumina Single End Adapter 1 (95% over 22bp)
TGCCGACTTAGCTCAGTAGGTAGAGCAACTGACTGTAGGCACCATCAATT269630.1641185970662926No Hit
AACTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA262480.15976652953291726Illumina Single End Adapter 1 (95% over 22bp)
AACTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATTC259640.15803787613504508No Hit
AGCGCACGCAGGCGGTTTGTTAAGTCAGATGCTGTAGGCACCATCAATTC257140.15651617420029848No Hit
AGCTCAGTTGGTAGAGCAGCGCACTGTAGGCACCATCAATTCGTATGCCG251800.15326581886767968No Hit
TACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGGCACCAT241860.14721553197512713No Hit
TTAGGTTAATGAGGCGAACCGGGGGAACTGACTGTAGGCACCATCAATTC238060.14490254503431224No Hit
TAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTCTGTAGGCACCATCAAT230370.14022178988303163No Hit
AAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTCTGTAGGCACCATCAAT230120.14006961968955697No Hit
AGACCGCCAGCTAAGGTCCCAAAGTCATGGTCTGTAGGCACCATCAATTC226110.1376288097862234No Hit
TCCCCATGCGAGAGTAGGGAACTGCCAGGCATCTGTAGGCACCATCAATT216130.13155417566271488No Hit
TTTCCAAAGGAATTCGCTCCACTGTGCTGTAGGCACCATCAATTCGTATG214430.1305194183470872No Hit
AGCTCAGTAGGTAGAGCAACTGACCTGTAGGCACCATCAATTCGTATGCC211950.12900989002781854No Hit
TTTCGCCATTTAAAGTGGTACGCGAGCTGGGCTGTAGGCACCATCAATTC206160.12548562834694535No Hit
TGGGCGTTAAGTTGCAGGGTATAGACCCGCTGTAGGCACCATCAATTCGT205230.1249195552272196No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCCTCTGTAGGCACCATCAATTC204320.12436565572297184No Hit
CGGTGGTTCAAGTCCACTCAGGCCTCTGTAGGCACCATCAATTCGTATGC202390.12319090182934746No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATT196510.1196118588788234Illumina Single End Adapter 1 (95% over 22bp)
AAAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTGTAGGCACCATCAATT195770.11916143510613841No Hit
ATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCACTGTAGGCACCATCAAT192820.1173658268231374No Hit
TGCCGATATAGCTCAGTTGGTAGAGCAGCGCACTGTAGGCACCATCAATT190750.1161058576211672No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGA184270.11216160620630396Illumina Single End Adapter 1 (95% over 22bp)
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGT179880.10948949760888889Illumina Single End Adapter 1 (95% over 22bp)
GCCGATACAGCTCAGTTGGTAGAGCAGCGCACTGTAGGCACCATCAATTC178450.10861908410221381No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACTCCCTGTAGGCACCATCAATTC176830.10763302124849801No Hit
TAGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATTCGT176630.10751128509371827No Hit
AGACCGCCAGCTAAGGTCCCAAAGTCATGGTTCTGTAGGCACCATCAATT173530.10562437469463246No Hit
ACGATGTCGACTTGGAGGTTGTGCCCCTGTAGGCACCATCAATTCGTATG172760.10515569049873051No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATG172660.10509482242134065Illumina Single End Adapter 1 (95% over 22bp)
TCAAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTGTAGGCACCATCAAT171910.10463831184091665No Hit
ACGATGTCGACTTGGAGGTTGTGCCCTGTAGGCACCATCAATTCGTATGC171740.10453483610935388No Hit
GCGGGAGTGGCGAAATTGGTAGACGCACCAGACTGTAGGCACCATCAATT167070.1016922968952472No Hit
CGCTGTGCGGGTTCAAGTCCCGCTCCGGGTACCACTGTAGGCACCATCAA166730.10148534543212165No Hit
ACCTGCGGTTGGATCACCTCCCTGTAGGCACCATCAATTCGTATGCCGTC164510.10013407411406665No Hit
AAGTCCCCCCCCTCGCACCACTGTAGGCACCATCAATTCGTATGCCGTCT164480.1001158136908497No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)