FastQCFastQC Report
Tue 4 Jul 2023
SRR6001747.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6001747.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11769741
Total Bases600.2 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTATG6549855.564990767426403No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT3092572.627559943757471No Hit
AGACCGCCAGCTAAGGTCCCAAAGTCATGGCTGTAGGCACCATCAATTCG1917201.6289228454559874No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATTCG1644901.3975668623464186No Hit
TAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTAT1581651.3438273620464545No Hit
ACGGGGGCCCGCACAAGCGGTGGAGCATGTGGCTGTAGGCACCATCAATT1338081.1368814318004108No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCTGTAGGCACCATCAATT1166440.99104984553186No Hit
AGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATTCGTA1134290.9637340362884791No Hit
ACCGCCAGCTAAGGTCCCAAAGTCATGGCTGTAGGCACCATCAATTCGTA1107490.9409637816159252No Hit
ACCGGAGTGGACGCATCACTGGTGTTCGGGTCTGTAGGCACCATCAATTC1090080.9261716124424488No Hit
CATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT818860.6957332366107292No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA793930.6745518019470437Illumina Single End Adapter 1 (95% over 22bp)
TTCGCCATTTAAAGTGGTACGCGAGCTGGGCTGTAGGCACCATCAATTCG702300.596699621512487No Hit
AAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTAT627800.5334017120682605No Hit
TAGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATTCGT566810.48158238996083264No Hit
AACTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATTC562910.478268808124155No Hit
AACGATGTCGACTTGGAGGTTGTGCCCTGTAGGCACCATCAATTCGTATG534340.4539946970795704No Hit
TAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTATG533860.4535868716227486No Hit
ACGATGTCGACTTGGAGGTTGTGCCCTGTAGGCACCATCAATTCGTATGC514160.43684903516568463No Hit
ACGGTGCTGAAGCAACAAATGCCCTGCTTCCCTGTAGGCACCATCAATTC496650.4219719023553704No Hit
AAGGCTGAGGCGTGATGACGAGGCACTGTAGGCACCATCAATTCGTATGC481140.408794042281814No Hit
ACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCTGTAGGCACCATCAAT468360.39793568949393193No Hit
AGCACTGTGCAAACACGAAAGTGGACGTATACGGTGCTGTAGGCACCATC454000.3857349112440112No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACTCCCTGTAGGCACCATCAATTC445820.378784885750672No Hit
TAGACCGCCAGCTAAGGTCCCAAAGTCATGGCTGTAGGCACCATCAATTC441160.3748255802740264No Hit
TCAAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTCTGTAGGCACCATCA439850.3737125566314501No Hit
TGGGCGTTAAGTTGCAGGGTATAGACCCGCTGTAGGCACCATCAATTCGT439050.373032847536747No Hit
AGACCGCCAGCTAAGGTCCCAAAGTCATGGTTAAGTGGCTGTAGGCACCA422900.3593112201874281No Hit
TATTCATCTCCGGGGGTAGAGCCTGTAGGCACCATCAATTCGTATGCCGT383150.3255381745443676No Hit
ATGGCCAGGCTGTCTCCACCCGAGACTCAGTGCTGTAGGCACCATCAATT377210.3204913345161971No Hit
AAAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTCTGTAGGCACCATCAA376990.32030441451515373No Hit
CAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTAT374220.31795092177474427No Hit
TTCGCCATTTAAAGTGGTACGCGAGCTGGCTGTAGGCACCATCAATTCGT363090.3084944689946873No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATTCG359730.30563969079693426No Hit
ATGGCACTGCCCGGTAGCTAAATGCGGAAGCTGTAGGCACCATCAATTCG350150.29750017438786464No Hit
AACGATGTCGACTTGGAGGTTGTGCCCCTGTAGGCACCATCAATTCGTAT349690.29710934165841035No Hit
AGCGCACGCAGGCGGTTTGTTAAGTCAGATGCTGTAGGCACCATCAATTC344430.29264025436073743No Hit
TACTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT336310.2857412070495009No Hit
ATAGGGGAGCCGAAGGGAAACCGAGTCTTCTGTAGGCACCATCAATTCGT330150.280507447020287No Hit
TACGGTGCTGAAGCAACAAATGCCCTGCTTCCCTGTAGGCACCATCAATT320170.2720280760638658No Hit
CATTCATCTCCGGGGGTAGAGCACTGTTTCGGCTGTAGGCACCATCAATT319330.27131438151442755No Hit
ATAAAGTGCGTCGTAGTCCGGATTGGAGTCCTGTAGGCACCATCAATTCG314600.26729560149199544No Hit
TTTCGCCATTTAAAGTGGTACGCGAGCTGGGCTGTAGGCACCATCAATTC308840.26240169601013313No Hit
AGGTAACATCAAATCGTACCCCAAACCGCTGTAGGCACCATCAATTCGTA307710.261441606913865No Hit
TTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATTCGT305870.25987827599604785No Hit
GGCTACGTAGCTCAGTTGGTTAGAGCACATCCTGTAGGCACCATCAATTC293760.2495891795749796No Hit
ACGATGTCGACTTGGAGGTTGTGCCCCTGTAGGCACCATCAATTCGTATG283110.24054055225174453No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCCTCTGTAGGCACCATCAATTC277820.23604597586302026No Hit
AAGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATTCGT275380.2339728631241758No Hit
ACGGGGCTAAACCATGCACCGAAGCTGCGGCACTGTAGGCACCATCAATT270430.22976716310070033No Hit
ATCACGGGAGACACACGGCGGGTGCTGTAGGCACCATCAATTCGTATGCC269960.22936783400756225No Hit
ATAAAGTGCGTCGTAGTCCGGATTGGAGCTGTAGGCACCATCAATTCGTA269480.22896000855074042No Hit
ACCGCCAGCTAAGGTCCCAAAGTCATGGTTAAGTGGCTGTAGGCACCATC263980.22428700852465655No Hit
TGCCGACTTAGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATTC263040.22348835033838044No Hit
TAAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTCTGTAGGCACCATCAA262280.22284262669841248No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAATT256470.2179062393981312No Hit
ACACGTGCTACAATGGCGCATACAAAGAGCTGTAGGCACCATCAATTCGT243170.20660607569869208No Hit
ACATCCTGGGGCTGAAGTAGGTCCCAAGGGTATGGCTGCTGTAGGCACCA228500.19414191017457394No Hit
TTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGCTGTAGGCACCATCA224180.19047148106317718No Hit
TAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTCTGTAGGCACCATCAAT219790.1867415774059939No Hit
TACCGCCAGCTAAGGTCCCAAAGTCATGGCTGTAGGCACCATCAATTCGT216280.18375935375298402No Hit
TAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGCTGTAGGCACCATCAAT215670.1832410755682729No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACCTGTAGGCACCATCAATT212790.18079412282734175No Hit
TAAGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATTCG211740.1799020046405439No Hit
AGGTTAATGAGGCGAACCGGGGGAACTGACTGTAGGCACCATCAATTCGT209820.17827070281325647No Hit
AGACCGCCAGCTAAGGTCCCAAAGTCATGGTTCTGTAGGCACCATCAATT205470.17457478461080836No Hit
CAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTATG199870.1698168209478866No Hit
ACACACGTGCTACAATGGCGCATACAAAGCTGTAGGCACCATCAATTCGT193200.1641497463707995No Hit
AGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCCTGTAGGCACCATCA191660.162841306363496No Hit
TCCCCATGCGAGAGTAGGGAACTGCCAGGCATCTGTAGGCACCATCAATT187400.15922185543420198No Hit
AACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCTGTAGGCACCATCAA185770.15783694815374444No Hit
GCCGATATAGCTCAGTTGGTAGAGCAGCGCACTGTAGGCACCATCAATTC182500.15505863722914548No Hit
GATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT180750.15357177358448246No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTAGGCACCATCAATTC179470.1524842390329575No Hit
CAGACCGCCAGCTAAGGTCCCAAAGTCATGGCTGTAGGCACCATCAATTC178480.1516430990282624No Hit
TTGGCCAGGCTGTCTCCACCCGAGACTCAGTGCTGTAGGCACCATCAATT176190.14969743174467476No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGCTGTAGGCACCATCAATTCG175790.1493575771973232No Hit
AGACCGCCAGCTAAGGTCCCAAAGTCATGGTCTGTAGGCACCATCAATTC171510.1457211335406616No Hit
AGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATTCGTATGCCGT170310.14470156989860694No Hit
ACCCAGACCGCCAGCTAAGGTCCCAAAGTCATGGCTGTAGGCACCATCAA168820.1434356117097224No Hit
TACACACGTGCTACAATGGCGCATACAAAGCTGTAGGCACCATCAATTCG168090.14281537716080583No Hit
ACCATGCACCGAAGCTGCGGCAGCGACGCCTGTAGGCACCATCAATTCGT166750.14167686442717814No Hit
TTTCGCCATTTAAAGTGGTACGCGAGCTGGCTGTAGGCACCATCAATTCG165730.1408102353314317No Hit
AAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATTCGTATGCC162010.13764958804106223No Hit
GCCGACTCAGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATTCG160970.1367659662179482No Hit
TTGGGAAACAGGTTAATATTCCTGTACTTGGTGTTACCTGTAGGCACCAT160810.1366300243990076No Hit
ACACACGTGCTACAATGGCGCATACAAAGAGCTGTAGGCACCATCAATTC157300.13364780074599772No Hit
CGGCACGTAGCGCAGCCTGGTAGCGCACCGCTGTAGGCACCATCAATTCG156430.1329086171055081No Hit
AAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTCTGTAGGCACCATCAAT150760.12809117889679986No Hit
AATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT149760.127241542528421No Hit
TTGGGAAACAGGTTAATATTCCTGTACTTGGTGCTGTAGGCACCATCAAT148980.12657882616108543No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCCTGTAGGCACCATCAAT147440.12527038615378197No Hit
TATAGGGGAGCCGAAGGGAAACCGAGTCTTCTGTAGGCACCATCAATTCG145910.12397044251016229No Hit
AACTTGCCCCGAGATGAGTTCTCCCTGACTCCCTGTAGGCACCATCAATT143790.12216921340919906No Hit
TTAGGTTAATGAGGCGAACCGGGGGAACTGACTGTAGGCACCATCAATTC143400.12183785522553131No Hit
TGCCGACTCAGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATTC141380.12012158976140597No Hit
TACTCATCTCCGGGGGTAGAGCACTGTTTCGGCTGTAGGCACCATCAATT140840.11966278612248137No Hit
TTAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTA139580.11859224429832398No Hit
TTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCTGTAGGCACCATCAAT137670.11696943883472032No Hit
ACGGTGCTGAAGCAACAAATGCCCTGCTGTAGGCACCATCAATTCGTATG134400.1141911279101214No Hit
ACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCGCTGTAGGCACCATCA132580.11264478971967182No Hit
AACGGGGCTAAACCATGCACCGAAGCTGCGGCACTGTAGGCACCATCAAT131570.11178665698760916No Hit
ACGGGGGCCCGCACAAGCGGTGGAGCATGTGCTGTAGGCACCATCAATTC131450.1116847006234037No Hit
CAGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATTCGT130150.11058017334451115No Hit
TAACCCAGACCGCCAGCTAAGGTCCCAAAGTCATGGCTGTAGGCACCATC129340.10989196788612426No Hit
ATGGGAAACAGGTTAATATTCCTGTACTTGGTGCTGTAGGCACCATCAAT124830.1060601078647355No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACTCCTGTAGGCACCATCAATTCG124660.1059156696821111No Hit
TACACACGTGCTACAATGGCGCATACAAAGAGCTGTAGGCACCATCAATT124220.10554182968002439No Hit
TTTGCCCCGAGATGAGTTCTCCCTGACTCCCTGTAGGCACCATCAATTCG124090.10543137695213514No Hit
TCGCCATTTAAAGTGGTACGCGAGCTGGGCTGTAGGCACCATCAATTCGT122140.10377458603379633No Hit
ACACGTGCTACAATGGCGCATACAAAGCTGTAGGCACCATCAATTCGTAT120230.10215178057019267No Hit
TACCCAGACCGCCAGCTAAGGTCCCAAAGTCATGGCTGTAGGCACCATCA119410.10145507874812199No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)