FastQCFastQC Report
Tue 4 Jul 2023
SRR6001748.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6001748.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11622124
Total Bases592.7 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTATG4767614.102184764161869No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT2738592.3563593023099734No Hit
AGACCGCCAGCTAAGGTCCCAAAGTCATGGCTGTAGGCACCATCAATTCG1560741.3429042746403326No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATTCG1449261.2469837699201969No Hit
ACGGGGGCCCGCACAAGCGGTGGAGCATGTGGCTGTAGGCACCATCAATT1379601.187046360888939No Hit
ACCGGAGTGGACGCATCACTGGTGTTCGGGTCTGTAGGCACCATCAATTC1209121.04036060878373No Hit
TAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTAT1199531.0321091050138513No Hit
ACCGCCAGCTAAGGTCCCAAAGTCATGGCTGTAGGCACCATCAATTCGTA1038920.8939157764966198No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCTGTAGGCACCATCAATT998990.8595588895799082No Hit
AGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATTCGTA978700.8421008070469735No Hit
TTCGCCATTTAAAGTGGTACGCGAGCTGGGCTGTAGGCACCATCAATTCG809540.6965508197985153No Hit
CATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT780840.6718565384434032No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATTCG735230.6326124209309761No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA658880.5669187491030039Illumina Single End Adapter 1 (95% over 22bp)
ACGATGTCGACTTGGAGGTTGTGCCCTGTAGGCACCATCAATTCGTATGC569710.4901943913178004No Hit
GGCTACGTAGCTCAGTTGGTTAGAGCACATCCTGTAGGCACCATCAATTC502600.4324510734870838No Hit
TGGGCGTTAAGTTGCAGGGTATAGACCCGCTGTAGGCACCATCAATTCGT495270.42614413682042973No Hit
ACGGTGCTGAAGCAACAAATGCCCTGCTTCCCTGTAGGCACCATCAATTC493780.4248620992169762No Hit
AACGATGTCGACTTGGAGGTTGTGCCCTGTAGGCACCATCAATTCGTATG465850.4008303473616355No Hit
AAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTAT462680.39810279084959No Hit
TAGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATTCGT447420.38497266076321335No Hit
AACTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATTC446600.384267109867353No Hit
ACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCTGTAGGCACCATCAAT430680.37056909735260096No Hit
TTCGCCATTTAAAGTGGTACGCGAGCTGGCTGTAGGCACCATCAATTCGT416140.3580584753699066No Hit
TCAAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTCTGTAGGCACCATCA402700.34649432410117115No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACTCCCTGTAGGCACCATCAATTC398170.34259658561550366No Hit
TAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTATG382160.3288211345877914No Hit
AAGGCTGAGGCGTGATGACGAGGCACTGTAGGCACCATCAATTCGTATGC365830.31477034662510917No Hit
TAGACCGCCAGCTAAGGTCCCAAAGTCATGGCTGTAGGCACCATCAATTC363550.31280857096344866No Hit
AGCACTGTGCAAACACGAAAGTGGACGTATACGGTGCTGTAGGCACCATC360880.31051122841229367No Hit
AGACCGCCAGCTAAGGTCCCAAAGTCATGGTTAAGTGGCTGTAGGCACCA358390.30836876288705917No Hit
TATTCATCTCCGGGGGTAGAGCCTGTAGGCACCATCAATTCGTATGCCGT327840.2820826898766525No Hit
GCCGACTCAGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATTCG317540.27322028228230916No Hit
ACGATGTCGACTTGGAGGTTGTGCCCCTGTAGGCACCATCAATTCGTATG314720.27079387554288703No Hit
ATGGCCAGGCTGTCTCCACCCGAGACTCAGTGCTGTAGGCACCATCAATT311170.2677393564205648No Hit
TGCCGACTTAGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATTC310530.267188682550625No Hit
TACTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT305920.263222109831215No Hit
TTTCGCCATTTAAAGTGGTACGCGAGCTGGGCTGTAGGCACCATCAATTC305190.26259399744831496No Hit
AAAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTCTGTAGGCACCATCAA303290.2609591843969312No Hit
AACGATGTCGACTTGGAGGTTGTGCCCCTGTAGGCACCATCAATTCGTAT302270.26008154791671473No Hit
ATGGCACTGCCCGGTAGCTAAATGCGGAAGCTGTAGGCACCATCAATTCG296270.2549189803860292No Hit
CAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTAT295700.25442853647061414No Hit
CATTCATCTCCGGGGGTAGAGCACTGTTTCGGCTGTAGGCACCATCAATT294290.25321533310090305No Hit
TACGGTGCTGAAGCAACAAATGCCCTGCTTCCCTGTAGGCACCATCAATT273480.23530982804864242No Hit
GCCGATATAGCTCAGTTGGTAGAGCAGCGCACTGTAGGCACCATCAATTC273230.23509472106819718No Hit
ATAGGGGAGCCGAAGGGAAACCGAGTCTTCTGTAGGCACCATCAATTCGT268240.23080118573851044No Hit
AGGTAACATCAAATCGTACCCCAAACCGCTGTAGGCACCATCAATTCGTA267920.23052584880354057No Hit
AGCGCACGCAGGCGGTTTGTTAAGTCAGATGCTGTAGGCACCATCAATTC259710.22346173556571933No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTAGGCACCATCAATTC258930.2227906017867302No Hit
ACCGCCAGCTAAGGTCCCAAAGTCATGGTTAAGTGGCTGTAGGCACCATC255620.2199425853656354No Hit
TTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATTCGT248770.21404865410143617No Hit
ACGGGGCTAAACCATGCACCGAAGCTGCGGCACTGTAGGCACCATCAATT248650.21394540275082247No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCCTCTGTAGGCACCATCAATTC247870.21327426897183335No Hit
TCCCCATGCGAGAGTAGGGAACTGCCAGGCATCTGTAGGCACCATCAATT239290.20589179740295319No Hit
ATAAAGTGCGTCGTAGTCCGGATTGGAGTCCTGTAGGCACCATCAATTCG238940.20559064763032986No Hit
TCGCCATTTAAAGTGGTACGCGAGCTGGGCTGTAGGCACCATCAATTCGT227490.19573874792593848No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAATT223670.19245191326473543No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGACTGTAGGCACCATCAATTC220110.1893887898631954No Hit
AAGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATTCGT215980.18583522254624027No Hit
TACCGCCAGCTAAGGTCCCAAAGTCATGGCTGTAGGCACCATCAATTCGT215250.1852071101633402No Hit
ATAAAGTGCGTCGTAGTCCGGATTGGAGCTGTAGGCACCATCAATTCGTA210150.18081892776225758No Hit
TAAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTCTGTAGGCACCATCAA206350.17754930165949012No Hit
ACACGTGCTACAATGGCGCATACAAAGAGCTGTAGGCACCATCAATTCGT205900.1771621090946887No Hit
AGGTTAATGAGGCGAACCGGGGGAACTGACTGTAGGCACCATCAATTCGT195220.16797273889006864No Hit
CGGCACGTAGCGCAGCCTGGTAGCGCACCGCTGTAGGCACCATCAATTCG187070.16096025132755423No Hit
GATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT185420.15954054525661573No Hit
TTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGCTGTAGGCACCATCA181650.1562967319915017No Hit
AGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCCTGTAGGCACCATCA175470.15097928743489572No Hit
TAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTCTGTAGGCACCATCAAT175320.15085022324662858No Hit
AGACCGCCAGCTAAGGTCCCAAAGTCATGGTTCTGTAGGCACCATCAATT170640.14682342057269396No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCCTGTAGGCACCATCAATTCGTA168770.14521442035896365No Hit
TAAGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATTCG167530.14414748973595534No Hit
ACCCAGACCGCCAGCTAAGGTCCCAAAGTCATGGCTGTAGGCACCATCAA167170.1438377356841142No Hit
GCGGGAATAGCTCAGTTGGTAGAGCACGACCCTGTAGGCACCATCAATTC167140.14381192284646077No Hit
ACACACGTGCTACAATGGCGCATACAAAGCTGTAGGCACCATCAATTCGT164300.141368307548603No Hit
TTTCGCCATTTAAAGTGGTACGCGAGCTGGCTGTAGGCACCATCAATTCG161290.13877841950404246No Hit
AACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCTGTAGGCACCATCAA159340.1371005850565697No Hit
TGCCGACTCAGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATTC157980.13593040308294765No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGTAGGCACCATCAATTCGTAT155230.13356422629805018No Hit
AGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATTCGTATGCCGT150310.12933092092288812No Hit
TTGGCCAGGCTGTCTCCACCCGAGACTCAGTGCTGTAGGCACCATCAATT148010.12735193670279202No Hit
ACCATGCACCGAAGCTGCGGCAGCGACGCCTGTAGGCACCATCAATTCGT146730.12625058896291247No Hit
TAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGCTGTAGGCACCATCAAT146380.12594943919028914No Hit
CAGACCGCCAGCTAAGGTCCCAAAGTCATGGCTGTAGGCACCATCAATTC146170.12576874932671514No Hit
CAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTATG144350.1242027705090739No Hit
TCGACTCCATGAAGTCGGAATCGCTAGTAATCGTGGCTGTAGGCACCATC140930.12126010701658319No Hit
TTCGCCATTTAAAGTGGTACGCGAGCTGCTGTAGGCACCATCAATTCGTA140400.12080408021803932No Hit
AGACCGCCAGCTAAGGTCCCAAAGTCATGGTCTGTAGGCACCATCAATTC139700.12020178067279268No Hit
GCCGATATAGCTCAGTTGGTAGAGCAGCGCCTGTAGGCACCATCAATTCG136950.11783560388789519No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCCTGTAGGCACCATCAAT136060.11706982303751018No Hit
TTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCTGTAGGCACCATCAAT135100.1162438122326005No Hit
ACGGGGGCCCGCACAAGCGGTGGAGCATGTGCTGTAGGCACCATCAATTC134720.11591684962232378No Hit
TACACACGTGCTACAATGGCGCATACAAAGCTGTAGGCACCATCAATTCG134510.11573615975874976No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGCCTGTAGGCACCATCAATT132180.11373136270100026No Hit
ACACACGTGCTACAATGGCGCATACAAAGAGCTGTAGGCACCATCAATTC131710.11332696157776323No Hit
ACGGTGCTGAAGCAACAAATGCCCTGCTGTAGGCACCATCAATTCGTATG130690.11244932509754671No Hit
ACATCCTGGGGCTGAAGTAGGTCCCAAGGGTATGGCTGCTGTAGGCACCA127670.10985083277376838No Hit
GGCTACGCAGCTCAGTTGGTTAGAGCACATCCTGTAGGCACCATCAATTC127600.10979060281924372No Hit
AATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT126860.10915388615712583No Hit
TTGGGAAACAGGTTAATATTCCTGTACTTGGTGTTACCTGTAGGCACCAT123410.10618540982698171No Hit
TACTCATCTCCGGGGGTAGAGCACTGTTTCGGCTGTAGGCACCATCAATT123270.10606494991793239No Hit
ACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCGCTGTAGGCACCATCA122960.1057982172621803No Hit
GCCGATACAGCTCAGTTGGTAGAGCAGCGCACTGTAGGCACCATCAATTC122370.10529056478832957No Hit
TATAGGGGAGCCGAAGGGAAACCGAGTCTTCTGTAGGCACCATCAATTCG121900.10488616366509254No Hit
AAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATTCGTATGCC121830.10482593371056788No Hit
TTGGGAAACAGGTTAATATTCCTGTACTTGGTGCTGTAGGCACCATCAAT120190.1034148319188472No Hit
AAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTCTGTAGGCACCATCAAT119950.10320832921761977No Hit
CGCAGGTTCGAATCCTGCACGACCCACCACTGTAGGCACCATCAATTCGT119520.10283834521125398No Hit
TGCCGATATAGCTCAGTTGGTAGAGCAGCGCACTGTAGGCACCATCAATT119230.10258882111393752No Hit
AACTTGCCCCGAGATGAGTTCTCCCTGACTCCCTGTAGGCACCATCAATT117820.10137561774422645No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)