FastQCFastQC Report
Tue 4 Jul 2023
SRR6001749.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6001749.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12537556
Total Bases639.4 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTATG4361793.4789794757447146No Hit
AGACCGCCAGCTAAGGTCCCAAAGTCATGGCTGTAGGCACCATCAATTCG2177361.7366702090901927No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT1463251.1670934909483157No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA1262071.0066315955039402Illumina Single End Adapter 1 (95% over 22bp)
ACCGCCAGCTAAGGTCCCAAAGTCATGGCTGTAGGCACCATCAATTCGTA1234170.9843784546206612No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACCTGTAGGCACCATCAATT1120990.894105677374442No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATTCG967720.7718569711672674No Hit
ACGGGGGCCCGCACAAGCGGTGGAGCATGTGGCTGTAGGCACCATCAATT965380.7699905787060891No Hit
ACCGGAGTGGACGCATCACTGGTGTTCGGGTCTGTAGGCACCATCAATTC948240.7563196527297665No Hit
AGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATTCGTA921640.7351033965471421No Hit
AGACCGCCAGCTAAGGTCCCAAAGTCATGGTTAAGTGGCTGTAGGCACCA817610.6521286923862992No Hit
AGCACTGTGCAAACACGAAAGTGGACGTATACGGTGCTGTAGGCACCATC705840.5629805362384822No Hit
TTTCGCCATTTAAAGTGGTACGCGAGCTGGGCTGTAGGCACCATCAATTC680830.5430324698051199No Hit
AAAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTCTGTAGGCACCATCAA679030.5415967832965213No Hit
TAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTAT601790.4799898800053216No Hit
TCAAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTCTGTAGGCACCATCA594690.47432689433251585No Hit
TAGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATTCGT576030.4594436108600432No Hit
AACGATGTCGACTTGGAGGTTGTGCCCCTGTAGGCACCATCAATTCGTAT561620.44795014275509515No Hit
ATCACGGGAGACACACGGCGGGTGCTGTAGGCACCATCAATTCGTATGCC546240.43568299914273567No Hit
AAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTAT527040.4203690097176834No Hit
AGGTAACATCAAATCGTACCCCAAACCGCTGTAGGCACCATCAATTCGTA477920.3811907201052582No Hit
ATGGCACTGCCCGGTAGCTAAATGCGGAAGCTGTAGGCACCATCAATTCG476630.38016181144076244No Hit
ACCGCCAGCTAAGGTCCCAAAGTCATGGTTAAGTGGCTGTAGGCACCATC471210.37583879984264873No Hit
TGGGCGTTAAGTTGCAGGGTATAGACCCGCTGTAGGCACCATCAATTCGT454300.36235132269798037No Hit
TAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTCTGTAGGCACCATCAAT445590.3554041952035947No Hit
ACGATGTCGACTTGGAGGTTGTGCCCCTGTAGGCACCATCAATTCGTATG445160.3550612256487628No Hit
TTCGCCATTTAAAGTGGTACGCGAGCTGGGCTGTAGGCACCATCAATTCG405740.32361969111045247No Hit
GCCGATATAGCTCAGTTGGTAGAGCAGCGCACTGTAGGCACCATCAATTC397810.3172946944364595No Hit
AGCGCACGCAGGCGGTTTGTTAAGTCAGATGCTGTAGGCACCATCAATTC396880.31655292307368355No Hit
ATAGGGGAGCCGAAGGGAAACCGAGTCTTCTGTAGGCACCATCAATTCGT392500.31305941923609354No Hit
GGCTACGTAGCTCAGTTGGTTAGAGCACATCCTGTAGGCACCATCAATTC384490.30667061427282954No Hit
AACGATGTCGACTTGGAGGTTGTGCCCTGTAGGCACCATCAATTCGTATG380620.30358388827934246No Hit
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACCTGTAGGCACCATCAATT376690.30044930606890213No Hit
ACGATGTCGACTTGGAGGTTGTGCCCTGTAGGCACCATCAATTCGTATGC373380.2978092381003124No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTAGGCACCATCAATTC363570.28998474662844975No Hit
AGACCGCCAGCTAAGGTCCCAAAGTCATGGTCTGTAGGCACCATCAATTC361050.28797478551641165No Hit
AGACCGCCAGCTAAGGTCCCAAAGTCATGGTTCTGTAGGCACCATCAATT359740.28692992477959817No Hit
ATGGCCAGGCTGTCTCCACCCGAGACTCAGTGCTGTAGGCACCATCAATT337890.2695022857724424No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCTGTAGGCACCATCAATT335980.26797886286609607No Hit
TTTCGCCATTTAAAGTGGTACGCGAGCTGGCTGTAGGCACCATCAATTCG334280.2666229367190862No Hit
TAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTATG330720.2637834678465245No Hit
ACCCAGACCGCCAGCTAAGGTCCCAAAGTCATGGCTGTAGGCACCATCAA325540.25965188111622395No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCCTCTGTAGGCACCATCAATTC315360.25153227630648267No Hit
AAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTCTGTAGGCACCATCAAT306230.24425015529342403No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACTCCCTGTAGGCACCATCAATTC299860.23916942026021656No Hit
ACGGTGCTGAAGCAACAAATGCCCTGCTTCCCTGTAGGCACCATCAATTC289190.23065898967869017No Hit
AACTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATTC274460.2189102884166579No Hit
TTGGGAAACAGGTTAATATTCCTGTACTTGGTGTTACCTGTAGGCACCAT262350.20925130862825256No Hit
AGGTTAATGAGGCGAACCGGGGGAACTGACTGTAGGCACCATCAATTCGT262100.20905190772428053No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATTCG253670.20232810924234357No Hit
AAGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATTCGT250950.20015862740712784No Hit
TACCCAGACCGCCAGCTAAGGTCCCAAAGTCATGGCTGTAGGCACCATCA249260.19881067729627688No Hit
ACATCCTGGGGCTGAAGTAGGTCCCAAGGGTATGGCTGCTGTAGGCACCA248270.19802104971654763No Hit
TGCCGATATAGCTCAGTTGGTAGAGCAGCGCACTGTAGGCACCATCAATT232820.18569807385107592No Hit
TAACCCAGACCGCCAGCTAAGGTCCCAAAGTCATGGCTGTAGGCACCATC229370.18294634137626184No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGACTGTAGGCACCATCAATTC226410.18058543467323296No Hit
TAAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTCTGTAGGCACCATCAA226190.18040996187773756No Hit
TAGACCGCCAGCTAAGGTCCCAAAGTCATGGCTGTAGGCACCATCAATTC223710.178431904910335No Hit
TTCGCCATTTAAAGTGGTACGCGAGCTGGCTGTAGGCACCATCAATTCGT217370.17337509798560422No Hit
ATAAAGTGCGTCGTAGTCCGGATTGGAGTCCTGTAGGCACCATCAATTCG217170.1732155772624266No Hit
ACACGTGCTACAATGGCGCATACAAAGAGCTGTAGGCACCATCAATTCGT216090.17235416535726739No Hit
TTAGGTTAATGAGGCGAACCGGGGGAACTGACTGTAGGCACCATCAATTC215720.1720590520193888No Hit
TTGGGCGTTAAGTTGCAGGGTATAGACCCGCTGTAGGCACCATCAATTCG215650.1720032197662766No Hit
ACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCTGTAGGCACCATCAAT215450.17184369904309899No Hit
TAGGGTGAGGTCCCCAGTTCGACTCTGGGTATCAGCACCACTGTAGGCAC214800.1713252566927717No Hit
CATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT208050.16594143228552677No Hit
AAGGCTGAGGCGTGATGACGAGGCACTGTAGGCACCATCAATTCGTATGC206560.16475300289785347No Hit
TAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGCTGTAGGCACCATCAAT203670.16244792844793673No Hit
TGCCGACTTAGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATTC198920.1586593112724681No Hit
TGCCGATACAGCTCAGTTGGTAGAGCAGCGCACTGTAGGCACCATCAATT186510.14876105039929632No Hit
TTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCCTGTAGGCACCAT183710.14652776027480954No Hit
TCCCCATGCGAGAGTAGGGAACTGCCAGGCATCTGTAGGCACCATCAATT182290.1453951631402484No Hit
TTGGCCAGGCTGTCTCCACCCGAGACTCAGTGCTGTAGGCACCATCAATT176700.1409365589274337No Hit
TATTCATCTCCGGGGGTAGAGCCTGTAGGCACCATCAATTCGTATGCCGT174710.13934932773181632No Hit
TTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCTGTAGGCACCATCAAT174450.1391419507916854No Hit
TACACACGTGCTACAATGGCGCATACAAAGAGCTGTAGGCACCATCAATT172760.13779400068083444No Hit
GCCGATACAGCTCAGTTGGTAGAGCAGCGCACTGTAGGCACCATCAATTC171480.13677306805249764No Hit
GCTGATATGGCTCAGTTGGTAGAGCGCACCCTCTGTAGGCACCATCAATT169320.13505024424217926No Hit
ACCGCCAGCTAAGGTCCCAAAGTCATGGTCTGTAGGCACCATCAATTCGT165840.13227458365888853No Hit
ACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCGACGCCTGTAGGCACC164840.1314769800430004No Hit
TTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATTCGT163570.13046402345082247No Hit
TAAGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATTCG161580.12887679225520507No Hit
GCTGATATGGCTCAGTTGGTAGAGCGCACCCCTGTAGGCACCATCAATTC161390.12872524756818632No Hit
TGCCGACTTAGCTCAGTAGGTAGAGCAACTGACTGTAGGCACCATCAATT158020.1260373233826433No Hit
TTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGCTGTAGGCACCATCA153570.12248798729194112No Hit
AGGTTAATGAGGCGAACCGGGGGAACTGCTGTAGGCACCATCAATTCGTA152770.1218499043992306No Hit
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACTGTAGGCACCATCAATTC148670.11857972957408924No Hit
TTAGGTTAATGAGGCGAACCGGGGGCTGTAGGCACCATCAATTCGTATGC148530.1184680650678649No Hit
AGTTCAGTCGGTAGAACGGCGGACCTGTAGGCACCATCAATTCGTATGCC147580.11771034163277118No Hit
TCGCCATTTAAAGTGGTACGCGAGCTGGGCTGTAGGCACCATCAATTCGT144260.11506229762802256No Hit
TACTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT143300.11429659815676994No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGTAGGCACCATCAATTCGTAT142320.11351494661319957No Hit
AGGTTAATGAGGCGAACCGGGGGCTGTAGGCACCATCAATTCGTATGCCG138820.1107233339575911No Hit
AGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCCTGTAGGCACCATCA135960.10844218761615103No Hit
TACACACGTGCTACAATGGCGCATACAAAGCTGTAGGCACCATCAATTCG134990.10766851210873953No Hit
AATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT134240.10707030939682344No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCCTGTAGGCACCATCAAT131080.10454988197061692No Hit
GCATCCGTAGCTCAGCTGGATAGAGTACTCGGCCTGTAGGCACCATCAAT129080.10295467473884065No Hit
AGGTAGCGCCTCGTGAATTCATCTCCGGGCTGTAGGCACCATCAATTCGT128700.10265158536480316No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACTCCTGTAGGCACCATCAATTCG127070.10135149147090548No Hit
TACCGCCAGCTAAGGTCCCAAAGTCATGGCTGTAGGCACCATCAATTCGT126330.10076126479514828No Hit
TAACGATGTCGACTTGGAGGTTGTGCCCCTGTAGGCACCATCAATTCGTA125450.1000593736131667No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)