FastQCFastQC Report
Tue 4 Jul 2023
SRR6001750.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6001750.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12383731
Total Bases631.5 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTATG3084472.4907437023623977No Hit
AGACCGCCAGCTAAGGTCCCAAAGTCATGGCTGTAGGCACCATCAATTCG1731231.3979874078337136No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT1231700.9946113978089479No Hit
ACCGCCAGCTAAGGTCCCAAAGTCATGGCTGTAGGCACCATCAATTCGTA1125820.9091121246092959No Hit
ACCGGAGTGGACGCATCACTGGTGTTCGGGTCTGTAGGCACCATCAATTC1039170.8391412894869891No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA1027100.8293946307457745Illumina Single End Adapter 1 (95% over 22bp)
ACGGGGGCCCGCACAAGCGGTGGAGCATGTGGCTGTAGGCACCATCAATT968070.7817272516659155No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATTCG831650.6715665900688573No Hit
AGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATTCGTA763750.6167365877052723No Hit
GGCTACGTAGCTCAGTTGGTTAGAGCACATCCTGTAGGCACCATCAATTC666200.5379638818058952No Hit
AGACCGCCAGCTAAGGTCCCAAAGTCATGGTTAAGTGGCTGTAGGCACCA661280.5339909272900065No Hit
TTTCGCCATTTAAAGTGGTACGCGAGCTGGGCTGTAGGCACCATCAATTC636710.5141503800429773No Hit
GCCGATATAGCTCAGTTGGTAGAGCAGCGCACTGTAGGCACCATCAATTC579010.4675569907001372No Hit
AGCACTGTGCAAACACGAAAGTGGACGTATACGGTGCTGTAGGCACCATC556880.449686770489443No Hit
TCAAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTCTGTAGGCACCATCA538740.4350385194898048No Hit
AAAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTCTGTAGGCACCATCAA526480.42513843364330184No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTAGGCACCATCAATTC508950.41098276440274745No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATTCG505950.4085602311613519No Hit
TGGGCGTTAAGTTGCAGGGTATAGACCCGCTGTAGGCACCATCAATTCGT497310.40158333542613284No Hit
ACGATGTCGACTTGGAGGTTGTGCCCCTGTAGGCACCATCAATTCGTATG475720.3841491711988899No Hit
AACGATGTCGACTTGGAGGTTGTGCCCCTGTAGGCACCATCAATTCGTAT474210.38292982946738746No Hit
TAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTAT444020.35855106994814406No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGACTGTAGGCACCATCAATTC439200.35465886654030193No Hit
TAGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATTCGT436100.35215558219085996No Hit
ACCGCCAGCTAAGGTCCCAAAGTCATGGTTAAGTGGCTGTAGGCACCATC434510.35087163957292034No Hit
TTCGCCATTTAAAGTGGTACGCGAGCTGGGCTGTAGGCACCATCAATTCG434360.3507505129108505No Hit
ACGATGTCGACTTGGAGGTTGTGCCCTGTAGGCACCATCAATTCGTATGC407720.3292384177272584No Hit
AGGTAACATCAAATCGTACCCCAAACCGCTGTAGGCACCATCAATTCGTA406060.32789794933368627No Hit
AAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTAT384410.3104153344416154No Hit
ATGGCACTGCCCGGTAGCTAAATGCGGAAGCTGTAGGCACCATCAATTCG381400.30798472608941524No Hit
TAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTCTGTAGGCACCATCAAT340950.27532090288459915No Hit
AACGATGTCGACTTGGAGGTTGTGCCCTGTAGGCACCATCAATTCGTATG326550.26369274332590076No Hit
TTTCGCCATTTAAAGTGGTACGCGAGCTGGCTGTAGGCACCATCAATTCG315440.2547212952219327No Hit
ACCCAGACCGCCAGCTAAGGTCCCAAAGTCATGGCTGTAGGCACCATCAA312920.2526863672991605No Hit
ATAGGGGAGCCGAAGGGAAACCGAGTCTTCTGTAGGCACCATCAATTCGT305900.247017639514295No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACCTGTAGGCACCATCAATT291390.23530065373674544No Hit
AGCGCACGCAGGCGGTTTGTTAAGTCAGATGCTGTAGGCACCATCAATTC287340.2320302338608615No Hit
AGACCGCCAGCTAAGGTCCCAAAGTCATGGTCTGTAGGCACCATCAATTC287120.23185258142315915No Hit
AGACCGCCAGCTAAGGTCCCAAAGTCATGGTTCTGTAGGCACCATCAATT286540.23138422499648933No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGTAGGCACCATCAATTCGTAT286090.23102084501028003No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCTGTAGGCACCATCAATT276970.22365634395643766No Hit
ACGGTGCTGAAGCAACAAATGCCCTGCTTCCCTGTAGGCACCATCAATTC272660.2201759711996328No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCCTCTGTAGGCACCATCAATTC271590.21931193434353508No Hit
ATGGCCAGGCTGTCTCCACCCGAGACTCAGTGCTGTAGGCACCATCAATT268350.21669559844282793No Hit
TGCCGATATAGCTCAGTTGGTAGAGCAGCGCACTGTAGGCACCATCAATT266300.21504020072787433No Hit
TCGCCATTTAAAGTGGTACGCGAGCTGGGCTGTAGGCACCATCAATTCGT263380.21268226837291604No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACTCCCTGTAGGCACCATCAATTC258720.20891926673794833No Hit
TTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCCTGTAGGCACCAT250770.20249955364825029No Hit
AAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTCTGTAGGCACCATCAAT238430.19253486691531008No Hit
TACCCAGACCGCCAGCTAAGGTCCCAAAGTCATGGCTGTAGGCACCATCA234880.18966820257965875No Hit
TAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTATG233760.18876379016953776No Hit
GCCGATACAGCTCAGTTGGTAGAGCAGCGCACTGTAGGCACCATCAATTC232310.18759289910286325No Hit
AGGTTAATGAGGCGAACCGGGGGAACTGACTGTAGGCACCATCAATTCGT231290.1867692378007888No Hit
TTCGCCATTTAAAGTGGTACGCGAGCTGGCTGTAGGCACCATCAATTCGT230770.1863493320389469No Hit
TGCCGACTTAGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATTC227880.18401562501640256No Hit
GCCGACTCAGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATTCG219450.1772083066080812No Hit
TCCCCATGCGAGAGTAGGGAACTGCCAGGCATCTGTAGGCACCATCAATT219100.17692567772991838No Hit
AACTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATTC213640.17251666723057857No Hit
TTGGGAAACAGGTTAATATTCCTGTACTTGGTGTTACCTGTAGGCACCAT198790.16052512768567082No Hit
GGCTACGCAGCTCAGTTGGTTAGAGCACATCCTGTAGGCACCATCAATTC193310.1560999669647217No Hit
ACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCTGTAGGCACCATCAAT193150.15597076519184727No Hit
CATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT190500.15383086082861458No Hit
AAGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATTCGT189010.15262766931872146No Hit
TGCCGATACAGCTCAGTTGGTAGAGCAGCGCACTGTAGGCACCATCAATT186590.15067349250399575No Hit
TTGGGCGTTAAGTTGCAGGGTATAGACCCGCTGTAGGCACCATCAATTCG185620.14989020675594456No Hit
TGCCGACTTAGCTCAGTAGGTAGAGCAACTGACTGTAGGCACCATCAATT180170.14548927136740938No Hit
TAGACCGCCAGCTAAGGTCCCAAAGTCATGGCTGTAGGCACCATCAATTC178270.14395500031452557No Hit
ACACGTGCTACAATGGCGCATACAAAGAGCTGTAGGCACCATCAATTCGT177400.14325246567452088No Hit
TAACCCAGACCGCCAGCTAAGGTCCCAAAGTCATGGCTGTAGGCACCATC175160.14144364085427888No Hit
TAAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTCTGTAGGCACCATCAA171310.138334723194488No Hit
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACTGTAGGCACCATCAATTC169460.13684082769562744No Hit
TAGGGTGAGGTCCCCAGTTCGACTCTGGGTATCAGCACCACTGTAGGCAC169430.13681660236321347No Hit
GGCTACGTAGCTCAGTTGGTTAGAGCACATCACTGTAGGCACCATCAATT168230.13584758906665528No Hit
TTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCTGTAGGCACCATCAAT168210.13583143884504598No Hit
TTAGGTTAATGAGGCGAACCGGGGGAACTGACTGTAGGCACCATCAATTC163620.13212496298571086No Hit
ATAAAGTGCGTCGTAGTCCGGATTGGAGTCCTGTAGGCACCATCAATTCG160840.12988008218201766No Hit
GCTGATATGGCTCAGTTGGTAGAGCGCACCCTCTGTAGGCACCATCAATT160300.1294440261985665No Hit
GCCGACTCAGCTCAGTAGGTAGAGCAACTGACTGTAGGCACCATCAATTC158850.128273135131892No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGCCTGTAGGCACCATCAATT153280.12377529841370101No Hit
AAGGCTGAGGCGTGATGACGAGGCACTGTAGGCACCATCAATTCGTATGC151540.12237022913369161No Hit
ACCGCCAGCTAAGGTCCCAAAGTCATGGTCTGTAGGCACCATCAATTCGT150350.12140929094793806No Hit
GCTGATATGGCTCAGTTGGTAGAGCGCACCCCTGTAGGCACCATCAATTC148560.11996384611390541No Hit
TATTCATCTCCGGGGGTAGAGCCTGTAGGCACCATCAATTCGTATGCCGT146610.11838919950699833No Hit
TTGGCCAGGCTGTCTCCACCCGAGACTCAGTGCTGTAGGCACCATCAATT145130.11719408310790988No Hit
ACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCGACGCCTGTAGGCACC143010.11548215961732372No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGACCTGTAGGCACCATCAATT141260.11406901522650968No Hit
AGGTAGCGCCTCGTGAATTCATCTCCGGGCTGTAGGCACCATCAATTCGT140810.11370563524030035No Hit
AGGTTAATGAGGCGAACCGGGGGAACTGCTGTAGGCACCATCAATTCGTA139350.11252666906282122No Hit
TGCCGACTTAGCTCAGTAGGTAGAGCAACTGTAGGCACCATCAATTCGTA138330.11170300776074674No Hit
ACATCCTGGGGCTGAAGTAGGTCCCAAGGGTATGGCTGCTGTAGGCACCA137350.11091164690189088No Hit
TACACACGTGCTACAATGGCGCATACAAAGAGCTGTAGGCACCATCAATT136600.110306013591542No Hit
GCATCCGTAGCTCAGCTGGATAGAGTACTCGGCCTGTAGGCACCATCAAT136190.10997493404855127No Hit
TAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGCTGTAGGCACCATCAAT134740.10880404298187678No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACCTGTAGGGACCATCAATT133880.10810958345267674No Hit
GATCGTCGGACTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAA133450.10776235368807673Illumina Single End Adapter 1 (95% over 22bp)
TTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATTCGT129680.10471803691472303No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)