FastQCFastQC Report
Mon 10 Jul 2023
SRR6051823.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6051823.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117529960
Total Bases5.8 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGGGCACGTCTGCCTGGGTGTCACATGGAATTCTCGGGTGCCAAGGAA2312211019.67337519726885RNA PCR Primer, Index 1 (100% over 23bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGC87894757.478497397599727RNA PCR Primer, Index 3 (100% over 50bp)
ACCCCTGTTTTTGGTCCCAAGGCTCGCTGGAATTCTCGGGTGCCAAGGAA61098155.198517041952537RNA PCR Primer, Index 1 (100% over 23bp)
ACGCATTTATTAGATAAAAGGTCGACGTGGAATTCTCGGGTGCCAAGGAA43722173.720087201595236RNA PCR Primer, Index 1 (100% over 23bp)
CCGAGGGCACGTCTGCCTGGGTGTCACATGGAATTCTCGGGTGCCAAGGA38556253.2805465091624297RNA PCR Primer, Index 1 (100% over 22bp)
CCGAGGGCACGTCTGCCTGGGTGTCTGGAATTCTCGGGTGCCAAGGAACT20670751.7587643184767527RNA PCR Primer, Index 1 (100% over 25bp)
CGAGGGCACGTCTGCCTGGGTGTCTGGAATTCTCGGGTGCCAAGGAACTC20535551.7472608686329851RNA PCR Primer, Index 1 (100% over 26bp)
ACCCCTGTTTTTGGTCCCAAGGCTCGCTTGGAATTCTCGGGTGCCAAGGA16536271.4069833768342983RNA PCR Primer, Index 1 (100% over 22bp)
ACCCCTGTTTTTGGTCCCAAGGCTCGTGGAATTCTCGGGTGCCAAGGAAC9439660.8031705277530937RNA PCR Primer, Index 1 (100% over 24bp)
AACCCCTGTTTTTGGTCCCAAGGCTCGCTGGAATTCTCGGGTGCCAAGGA7199420.612560405874383RNA PCR Primer, Index 1 (100% over 22bp)
GAAGGTCACGGCGAGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC7135600.6071303010738709RNA PCR Primer, Index 1 (100% over 33bp)
ACGCATTTATTAGATAAAAGGTCGACTGGAATTCTCGGGTGCCAAGGAAC6112620.5200903667456366RNA PCR Primer, Index 1 (100% over 24bp)
ACCCCTGTTTTTGGTCCCAAGGCTCTGGAATTCTCGGGTGCCAAGGAACT4092080.348173350863048RNA PCR Primer, Index 1 (100% over 25bp)
TGCCGGCCGGGGGACGGACTGGGATGGAATTCTCGGGTGCCAAGGAACTC3961370.3370519312692696RNA PCR Primer, Index 1 (100% over 26bp)
ACGCATTTATTAGATAAAAGGTCTGGAATTCTCGGGTGCCAAGGAACTCC3950770.336150033574418RNA PCR Primer, Index 1 (100% over 27bp)
GAGGGCACGTCTGCCTGGGTGTCACATGGAATTCTCGGGTGCCAAGGAAC3583240.30487885812264376RNA PCR Primer, Index 1 (100% over 24bp)
AACCCCTGTTTTTGGTCCCAAGGCTCGTGGAATTCTCGGGTGCCAAGGAA3509240.29858259119632136RNA PCR Primer, Index 1 (100% over 23bp)
CCCCTGTTTTTGGTCCCAAGGCTCGCTTGGAATTCTCGGGTGCCAAGGAA3426380.29153247393260406RNA PCR Primer, Index 1 (100% over 23bp)
GAGGGCACGTCTGCCTGGGTGTCTGGAATTCTCGGGTGCCAAGGAACTCC3306650.2813452842151907RNA PCR Primer, Index 1 (100% over 27bp)
CGAGGGCACGTCTGCCTGGGTGTCACTGGAATTCTCGGGTGCCAAGGAAC3290730.2799907359791495RNA PCR Primer, Index 1 (100% over 24bp)
AACCCCTGTTTTTGGTCCCAAGGCTCTGGAATTCTCGGGTGCCAAGGAAC3106030.26427559406980144RNA PCR Primer, Index 1 (100% over 24bp)
AGGGAGAACTGCGAGGAGCCAACCCTTGGAATTCTCGGGTGCCAAGGAAC2573120.21893311288457853RNA PCR Primer, Index 1 (100% over 24bp)
CCCCTGTTTTTGGTCCCAAGGCTCGCTGGAATTCTCGGGTGCCAAGGAAC2309960.19654222633956484RNA PCR Primer, Index 1 (100% over 24bp)
ACGTCTGCCTGGGTGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC2200240.18720673435096888RNA PCR Primer, Index 1 (100% over 33bp)
GAGATCGGAAAGAACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT1853030.1576644797632876RNA PCR Primer, Index 3 (100% over 34bp)
TGGCCGAGGGCACGTCTGCCTGTGGAATTCTCGGGTGCCAAGGAACTCCA1721350.14646052802196136RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCACCTCGTATGC1683190.14321369632049563RNA PCR Primer, Index 3 (98% over 50bp)
GGTCACGGCGAGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTA1642440.13974649527660862RNA PCR Primer, Index 3 (100% over 36bp)
ATGTACACGGAGTCGACCCGCAACGCGTGGAATTCTCGGGTGCCAAGGAA1632070.13886416706004154RNA PCR Primer, Index 1 (100% over 23bp)
AGGGAGAACTGCGAGGAGCCAACCCTGGAATTCTCGGGTGCCAAGGAACT1615250.1374330426046261RNA PCR Primer, Index 1 (100% over 25bp)
GAAAAGGACTTTGAAAAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1591910.1354471659821887RNA PCR Primer, Index 1 (100% over 32bp)
ACGCATTTATTAGATAAAAGGTCGACGCTGGAATTCTCGGGTGCCAAGGA1578740.1343266006386797RNA PCR Primer, Index 1 (100% over 22bp)
GGGAGAGCACTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGG1526940.12991921379025398RNA PCR Primer, Index 3 (100% over 38bp)
CCGAGGGCACGTCTGCCTGGGTGTTGGAATTCTCGGGTGCCAAGGAACTC1472690.1253033694557541RNA PCR Primer, Index 1 (100% over 26bp)
AGGGCACGTCTGCCTGGGTGTCACATGGAATTCTCGGGTGCCAAGGAACT1269870.10804649299633896RNA PCR Primer, Index 1 (100% over 25bp)
TAAGTCCGTTCGGCGGAAGCGTCGTTCTGGAATTCTCGGGTGCCAAGGAA1178320.10025698979221978RNA PCR Primer, Index 1 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)