FastQCFastQC Report
Tue 4 Jul 2023
SRR6051824.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6051824.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129697201
Total Bases6.4 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGGGCACGTCTGCCTGGGTGTCACATGGAATTCTCGGGTGCCAAGGAA1630308712.570114755213568RNA PCR Primer, Index 1 (100% over 23bp)
ACCCCTGTTTTTGGTCCCAAGGCTCGCTGGAATTCTCGGGTGCCAAGGAA87896266.777035997870147RNA PCR Primer, Index 1 (100% over 23bp)
ACGCATTTATTAGATAAAAGGTCGACGTGGAATTCTCGGGTGCCAAGGAA63167444.8703780430851396RNA PCR Primer, Index 1 (100% over 23bp)
ACCCCTGTTTTTGGTCCCAAGGCTCGCTTGGAATTCTCGGGTGCCAAGGA31843342.4552064157498665RNA PCR Primer, Index 1 (100% over 22bp)
CGAGGGCACGTCTGCCTGGGTGTCTGGAATTCTCGGGTGCCAAGGAACTC28211912.175213480512968RNA PCR Primer, Index 1 (100% over 26bp)
CCGAGGGCACGTCTGCCTGGGTGTCACATGGAATTCTCGGGTGCCAAGGA23199041.7887078380357646RNA PCR Primer, Index 1 (100% over 22bp)
CCGAGGGCACGTCTGCCTGGGTGTCTGGAATTCTCGGGTGCCAAGGAACT20373921.57088355360884RNA PCR Primer, Index 1 (100% over 25bp)
CCCCTGTTTTTGGTCCCAAGGCTCGCTTGGAATTCTCGGGTGCCAAGGAA16981231.3092981089083027RNA PCR Primer, Index 1 (100% over 23bp)
GAAGGTCACGGCGAGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC15460581.192051939501763RNA PCR Primer, Index 1 (100% over 33bp)
AACCCCTGTTTTTGGTCCCAAGGCTCGCTGGAATTCTCGGGTGCCAAGGA15067831.1617698673389258RNA PCR Primer, Index 1 (100% over 22bp)
ACGTCTGCCTGGGTGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC9705440.7483153009601187RNA PCR Primer, Index 1 (100% over 33bp)
ACGCATTTATTAGATAAAAGGTCGACTGGAATTCTCGGGTGCCAAGGAAC7308710.5635210277205597RNA PCR Primer, Index 1 (100% over 24bp)
ACGCATTTATTAGATAAAAGGTCTGGAATTCTCGGGTGCCAAGGAACTCC7170420.5528585000072592RNA PCR Primer, Index 1 (100% over 27bp)
CGAGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCT6529620.503451111485436RNA PCR Primer, Index 11 (100% over 43bp)
CGAGGGCACGTCTGCCTGGGTGTCACTGGAATTCTCGGGTGCCAAGGAAC6490540.5004379392890677RNA PCR Primer, Index 1 (100% over 24bp)
GAGGGCACGTCTGCCTGGGTGTCTGGAATTCTCGGGTGCCAAGGAACTCC5193110.4004026270389598RNA PCR Primer, Index 1 (100% over 27bp)
CCGAGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATC5151370.3971843617504128RNA PCR Primer, Index 11 (100% over 42bp)
ACGCATTTATTAGATAAAAGGTCGACGCTGGAATTCTCGGGTGCCAAGGA4621220.35630838324722214RNA PCR Primer, Index 1 (100% over 22bp)
GCACGTCTGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTAC4055960.31272533013260634RNA PCR Primer, Index 11 (100% over 39bp)
TCCACTAGTAAACGTCACAAAGCAATTGTGGAATTCTCGGGTGCCAAGGA3983200.3071153401375254RNA PCR Primer, Index 1 (100% over 22bp)
CCCCTGTTTTTGGTCCCAAGGCTCGCTGGAATTCTCGGGTGCCAAGGAAC3273480.25239403585895426RNA PCR Primer, Index 1 (100% over 24bp)
ACCCCTGTTTTTGGTCCCAAGGCTCTGGAATTCTCGGGTGCCAAGGAACT2875900.2217395578182138RNA PCR Primer, Index 1 (100% over 25bp)
AGGGCACGTCTGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGC2607840.2010714171079143RNA PCR Primer, Index 11 (100% over 36bp)
AGGGAGAACTGCGAGGAGCCAACCCTTGGAATTCTCGGGTGCCAAGGAAC2213300.17065133117252085RNA PCR Primer, Index 1 (100% over 24bp)
AACCCCTGTTTTTGGTCCCAAGGCTCTGGAATTCTCGGGTGCCAAGGAAC2211980.17054955565309385RNA PCR Primer, Index 1 (100% over 24bp)
GCACGTCTGCCTGGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG2187000.16862353105060454RNA PCR Primer, Index 6 (100% over 34bp)
AACCCCTGTTTTTGGTCCCAAGGCTCGCTTGGAATTCTCGGGTGCCAAGG2164690.16690337056695617Illumina Small RNA Adapter 2 (100% over 21bp)
CGAGGGCACGTCTGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG1989620.15340500678962224RNA PCR Primer, Index 6 (100% over 34bp)
GGCACGTCTGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTA1897830.1463277530561357RNA PCR Primer, Index 11 (100% over 38bp)
ACGTCTGCCTGGGTGTCACATGGAATTCTCGGGTGCCAAGGAACTCCAGT1862640.143614510231412RNA PCR Primer, Index 1 (100% over 30bp)
GGGCACGTCTGCCTGGGTGTCACATGGAATTCTCGGGTGCCAAGGAACTC1807760.13938311590856922RNA PCR Primer, Index 1 (100% over 26bp)
AACCCCTGTTTTTGGTCCCAAGGCTCGTGGAATTCTCGGGTGCCAAGGAA1767950.13631365876585108RNA PCR Primer, Index 1 (100% over 23bp)
GAGGGCACGTCTGCCTGGGTGTCACATGGAATTCTCGGGTGCCAAGGAAC1635750.1261206862899069RNA PCR Primer, Index 1 (100% over 24bp)
CCGAGGGCACGTCTGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1621230.12500115557621017RNA PCR Primer, Index 1 (100% over 33bp)
CGAGGGCACGTCTGCCTGGGTGTCACACCGAGGGCACGTCTGCCTGGGTG1591160.12268267840259714No Hit
ACCCCTGTTTTTGGTCCCAAGGCTCGTGGAATTCTCGGGTGCCAAGGAAC1512250.116598507010186RNA PCR Primer, Index 1 (100% over 24bp)
CACGTCTGCCTGGGTGTCACATGGAATTCTCGGGTGCCAAGGAACTCCAG1494810.11525383651109017RNA PCR Primer, Index 1 (100% over 29bp)
GGGGGTCCCAGTTCCGAACCCGTCGGCTGGAATTCTCGGGTGCCAAGGAA1450480.11183587531700086RNA PCR Primer, Index 1 (100% over 23bp)
CCGAGGGCACGTCTGCCTGGGTGTCACTGGAATTCTCGGGTGCCAAGGAA1446190.1115051048788632RNA PCR Primer, Index 1 (100% over 23bp)
CGAGGGCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATC1395460.10759368662088553RNA PCR Primer, Index 11 (100% over 42bp)
ACCCCTGTTTTTGGTCCCAAGGCTCGCCTGGAATTCTCGGGTGCCAAGGA1369570.1055974985921246RNA PCR Primer, Index 1 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)