FastQCFastQC Report
Tue 4 Jul 2023
SRR6051825.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6051825.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121525721
Total Bases6 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGGGCACGTCTGCCTGGGTGTCACATGGAATTCTCGGGTGCCAAGGAA2938244024.177959824653087RNA PCR Primer, Index 1 (100% over 23bp)
CGAGGGCACGTCTGCCTGGGTGTCTGGAATTCTCGGGTGCCAAGGAACTC27349472.2505087626676166RNA PCR Primer, Index 1 (100% over 26bp)
ACGCTTTTATTAGATCAAAACCGGCCCGTGGAATTCTCGGGTGCCAAGGA25538392.1014802290290464RNA PCR Primer, Index 1 (100% over 22bp)
CGAGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGCATCT16643051.36950843517316RNA PCR Primer, Index 18 (100% over 43bp)
GAAGGTCACGGCGAGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC14978941.2325736376416974RNA PCR Primer, Index 1 (100% over 33bp)
CCGAGGGCACGTCTGCCTGGGTGTCACATGGAATTCTCGGGTGCCAAGGA14632531.2040685609262916RNA PCR Primer, Index 1 (100% over 22bp)
CGAGGGCACGTCTGCCTGGGTGTCACTGGAATTCTCGGGTGCCAAGGAAC9467910.779086922677052RNA PCR Primer, Index 1 (100% over 24bp)
CCGAGGGCACGTCTGCCTGGGTGTCTGGAATTCTCGGGTGCCAAGGAACT9006290.7411015483709823RNA PCR Primer, Index 1 (100% over 25bp)
AACGCGGCGTGTTCGAATTCTGATCGCTGGAATTCTCGGGTGCCAAGGAA7416360.6102708084323976RNA PCR Primer, Index 1 (100% over 23bp)
CGTGAACCATCGAGTCTTTGAACGCAATGGAATTCTCGGGTGCCAAGGAA4930870.40574702700179827RNA PCR Primer, Index 1 (100% over 23bp)
GCACGTCTGCCTGGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG4867040.40049464096575904RNA PCR Primer, Index 6 (100% over 34bp)
GGGCCCGTCTCGGGAAAACGATCGTCCGTGGAATTCTCGGGTGCCAAGGA4703920.38707196808155536RNA PCR Primer, Index 1 (100% over 22bp)
CCGAGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGCATC3958700.32574997024703933RNA PCR Primer, Index 18 (100% over 42bp)
GCACGTCTGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGC3732700.30715308407839026RNA PCR Primer, Index 18 (100% over 39bp)
CGAGGGCACGTCTGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG3333300.2742876135661849RNA PCR Primer, Index 6 (100% over 34bp)
CACGTCTGCCTGGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT3038190.25000386543684855RNA PCR Primer, Index 17 (100% over 35bp)
CGAGGGCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGCATC2950500.24278810902919884RNA PCR Primer, Index 18 (100% over 42bp)
AAACGCGGCGTGTTCGAATTCTGATCGCTGGAATTCTCGGGTGCCAAGGA2846590.23423765574696734RNA PCR Primer, Index 1 (100% over 22bp)
CCGGGAAGATGACCGAACTCGGCCGTTTGGAATTCTCGGGTGCCAAGGAA2693930.22167570600136574RNA PCR Primer, Index 1 (100% over 23bp)
GGGGGTCCCAGTTCCGAACCCGTCGGCTGGAATTCTCGGGTGCCAAGGAA2668880.21961441397249556RNA PCR Primer, Index 1 (100% over 23bp)
ACGTCTGCCTGGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTC2617210.21536263915685802RNA PCR Primer, Index 18 (100% over 36bp)
AGGGAGAACTGCGAGGAGCCAACCCTTGGAATTCTCGGGTGCCAAGGAAC2383600.19613954810438852RNA PCR Primer, Index 1 (100% over 24bp)
GAGGGCACGTCTGCCTGGGTGTCTGGAATTCTCGGGTGCCAAGGAACTCC2376030.1955166347048457RNA PCR Primer, Index 1 (100% over 27bp)
CGTGAACCATCGAGTCTTTGAACGCAAGTGGAATTCTCGGGTGCCAAGGA2366350.19472009550965758RNA PCR Primer, Index 1 (100% over 22bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCC2296090.1889386033759882RNA PCR Primer, Index 18 (100% over 37bp)
GGCCCGTCTCGGGAAAACGATCGTCCGTTGGAATTCTCGGGTGCCAAGGA2203130.18128919391475984RNA PCR Primer, Index 1 (100% over 22bp)
GGGAGAATCTTAGCGACAAAGGGCTGTGGAATTCTCGGGTGCCAAGGAAC2043720.16817180619730698RNA PCR Primer, Index 1 (100% over 24bp)
GGGCACGTCTGCCTGGGTGTCACATGGAATTCTCGGGTGCCAAGGAACTC2003350.16484987569010184RNA PCR Primer, Index 1 (100% over 26bp)
GAGGGCACGTCTGCCTGGGTGTCACATGGAATTCTCGGGTGCCAAGGAAC1989530.16371266787217825RNA PCR Primer, Index 1 (100% over 24bp)
CGAGGGCACGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTC1846440.15193820574000133RNA PCR Primer, Index 18 (100% over 36bp)
CCGGGAAGATGACCGAACTCGGCCGTTTTGGAATTCTCGGGTGCCAAGGA1802560.14832744748743354RNA PCR Primer, Index 1 (100% over 22bp)
CCGAGGGCACGTCTGCCTGGGTGTCACTGGAATTCTCGGGTGCCAAGGAA1676460.1379510433021829RNA PCR Primer, Index 1 (100% over 23bp)
CGAGGGCACGTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT1667910.137247488537838RNA PCR Primer, Index 17 (100% over 35bp)
GGTCACGGCGAGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTC1617780.13312243586688946RNA PCR Primer, Index 18 (100% over 36bp)
CCGAGGGCACGTCTGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1575540.1296466284697048RNA PCR Primer, Index 1 (100% over 33bp)
ACGCTTTTATTAGATCAAAACCGGCCTGGAATTCTCGGGTGCCAAGGAAC1427190.11743933615501857RNA PCR Primer, Index 1 (100% over 24bp)
ACGATGCCAGCTAGCGATCCGCCGGCGTGGAATTCTCGGGTGCCAAGGAA1382090.11372818763198285RNA PCR Primer, Index 1 (100% over 23bp)
CACGTCTGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGCA1359430.1118635617887015RNA PCR Primer, Index 18 (100% over 40bp)
AGGGCACGTCTGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTC1305790.1074496813723903RNA PCR Primer, Index 18 (100% over 36bp)
GGGAGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGCATCT1283970.1056541766989393RNA PCR Primer, Index 18 (100% over 43bp)
CCGAGGGCACGTCTGCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1223110.10064618337051463RNA PCR Primer, Index 1 (100% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)