FastQCFastQC Report
Tue 4 Jul 2023
SRR6051826.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6051826.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91701400
Total Bases4.5 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGGGCACGTCTGCCTGGGTGTCACATGGAATTCTCGGGTGCCAAGGAA85958669.37375656205903RNA PCR Primer, Index 1 (100% over 23bp)
ACCCCTGTTTTTGGTCCCAAGGCTCGCTGGAATTCTCGGGTGCCAAGGAA76674728.36134671880691RNA PCR Primer, Index 1 (100% over 23bp)
CCCCTGTTTTTGGTCCCAAGGCTCGCTGGAATTCTCGGGTGCCAAGGAAC39855804.346258617643787RNA PCR Primer, Index 1 (100% over 24bp)
CCCCTGTTTTTGGTCCCAAGGCTCGCTTGGAATTCTCGGGTGCCAAGGAA37051994.040504288920344RNA PCR Primer, Index 1 (100% over 23bp)
ACCCCTGTTTTTGGTCCCAAGGCTCGCTTGGAATTCTCGGGTGCCAAGGA15742181.716678262272986RNA PCR Primer, Index 1 (100% over 22bp)
ACCCCTGTTTTTGGTCCCAAGGCTCTGGAATTCTCGGGTGCCAAGGAACT14748801.60835058134336RNA PCR Primer, Index 1 (100% over 25bp)
CGAGGGCACGTCTGCCTGGGTGTCTGGAATTCTCGGGTGCCAAGGAACTC11723281.2784188681961235RNA PCR Primer, Index 1 (100% over 26bp)
AGGGAGAACTGCGAGGAGCCAACCCTGGAATTCTCGGGTGCCAAGGAACT10251441.1179153208129864RNA PCR Primer, Index 1 (100% over 25bp)
AACCCCTGTTTTTGGTCCCAAGGCTCTGGAATTCTCGGGTGCCAAGGAAC8776380.9570606337525926RNA PCR Primer, Index 1 (100% over 24bp)
GAAGGTCACGGCGAGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC7812150.851911748348444RNA PCR Primer, Index 1 (100% over 33bp)
CGAGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCT6334970.6908258761589245RNA PCR Primer, Index 19 (100% over 43bp)
AACCCCTGTTTTTGGTCCCAAGGCTCGCTGGAATTCTCGGGTGCCAAGGA5807580.6333142133053584RNA PCR Primer, Index 1 (100% over 22bp)
AGGGAGAACTGCGAGGAGCCAACCCTTGGAATTCTCGGGTGCCAAGGAAC4930310.5376482801789285RNA PCR Primer, Index 1 (100% over 24bp)
CGAGGGCACGTCTGCCTGGGTGTCACTGGAATTCTCGGGTGCCAAGGAAC4872080.5312983225992188RNA PCR Primer, Index 1 (100% over 24bp)
GGGGGTCCCAGTTCCGAACCCGTCGGCTGGAATTCTCGGGTGCCAAGGAA4560620.49733373754381066RNA PCR Primer, Index 1 (100% over 23bp)
CCCCTGTTTTTGGTCCCAAGGCTCGCCTGGAATTCTCGGGTGCCAAGGAA4386140.4783067652184154RNA PCR Primer, Index 1 (100% over 23bp)
ACCCCTGTTTTTGGTCCCAAGGCTCGTGGAATTCTCGGGTGCCAAGGAAC4179050.4557236857888757RNA PCR Primer, Index 1 (100% over 24bp)
ACGCATTTATTAGATAAAAGGTCTGGAATTCTCGGGTGCCAAGGAACTCC4053970.4420837631704641RNA PCR Primer, Index 1 (100% over 27bp)
ACGCATTTATTAGATAAAAGGTCGACGTGGAATTCTCGGGTGCCAAGGAA4004850.4367272473484592RNA PCR Primer, Index 1 (100% over 23bp)
ATGTACACGGAGTCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA3704310.4039534838072265RNA PCR Primer, Index 1 (100% over 32bp)
CCGAGGGCACGTCTGCCTGGGTGTCTGGAATTCTCGGGTGCCAAGGAACT3703260.40383898173855576RNA PCR Primer, Index 1 (100% over 25bp)
ACGCTTTTATTAGATCAAAACCGGCCCGTGGAATTCTCGGGTGCCAAGGA2790870.30434322703906375RNA PCR Primer, Index 1 (100% over 22bp)
CCGAGGGCACGTCTGCCTGGGTGTCACATGGAATTCTCGGGTGCCAAGGA2728220.29751127027504487RNA PCR Primer, Index 1 (100% over 22bp)
CCCTGTTTTTGGTCCCAAGGCTCGCTTGGAATTCTCGGGTGCCAAGGAAC2622880.28602398654764266RNA PCR Primer, Index 1 (100% over 24bp)
ATGTACACGGAGTCGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC2510310.2737482742902508RNA PCR Primer, Index 1 (100% over 33bp)
TTTTGGTCCCAAGGCTCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC2144540.23386120604483684RNA PCR Primer, Index 1 (100% over 31bp)
CGTGAACCATCGAGTCTTTGAACGCAATGGAATTCTCGGGTGCCAAGGAA2105760.22963226297526537RNA PCR Primer, Index 1 (100% over 23bp)
GAGGGCACGTCTGCCTGGGTGTCACATGGAATTCTCGGGTGCCAAGGAAC2051450.22370977978525955RNA PCR Primer, Index 1 (100% over 24bp)
ATGTACACGGAGTCGACCCGCAACGCGTGGAATTCTCGGGTGCCAAGGAA2031960.22158440329155282RNA PCR Primer, Index 1 (100% over 23bp)
GGGGTCCCAGTTCCGAACCCGTCGGCTGGAATTCTCGGGTGCCAAGGAAC1944690.21206764564117886RNA PCR Primer, Index 1 (100% over 24bp)
CTGTTTTTGGTCCCAAGGCTCGCTGGAATTCTCGGGTGCCAAGGAACTCC1922710.20967073567033875RNA PCR Primer, Index 1 (100% over 27bp)
CGTGAACCATCGAGTCTTTGAACGCATGGAATTCTCGGGTGCCAAGGAAC1647020.1796068544209794RNA PCR Primer, Index 1 (100% over 24bp)
GCACGTCTGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAA1625940.17730808908042844RNA PCR Primer, Index 19 (100% over 39bp)
AACGCGGCGTGTTCGAATTCTGATCGCTGGAATTCTCGGGTGCCAAGGAA1611110.1756908836724412RNA PCR Primer, Index 1 (100% over 23bp)
CCCCTGTTTTTGGTCCCAAGGCTCGTGGAATTCTCGGGTGCCAAGGAACT1602730.17477704811485975RNA PCR Primer, Index 1 (100% over 25bp)
ACCCCTGTTTTTGGTCCCAAGGCTTGGAATTCTCGGGTGCCAAGGAACTC1586890.17304970262177022RNA PCR Primer, Index 1 (100% over 26bp)
CCCCTGTTTTTGGTCCCAAGGCTCTGGAATTCTCGGGTGCCAAGGAACTC1562660.17040743107520712RNA PCR Primer, Index 1 (100% over 26bp)
GGGAGAACTGCGAGGAGCCAACCCTTGGAATTCTCGGGTGCCAAGGAACT1549250.1689450760838984RNA PCR Primer, Index 1 (100% over 25bp)
TGTTTTTGGTCCCAAGGCTCGCTGGAATTCTCGGGTGCCAAGGAACTCCA1361730.14849609711520217RNA PCR Primer, Index 1 (100% over 28bp)
GGTCACGGCGAGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTG1351130.14734017146957407RNA PCR Primer, Index 19 (100% over 36bp)
CGAGGGCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATC1313660.14325408336186796RNA PCR Primer, Index 19 (100% over 42bp)
GGCCCGTCTCGGGAAAACGATCGTCCGTGGAATTCTCGGGTGCCAAGGAA1284930.140121088663859RNA PCR Primer, Index 1 (100% over 23bp)
TTTGGTCCCAAGGCTCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1268690.1383501233350854RNA PCR Primer, Index 1 (100% over 32bp)
GGGCACGTCTGCCTGGGTGTCACATGGAATTCTCGGGTGCCAAGGAACTC1235920.13477656829666723RNA PCR Primer, Index 1 (100% over 26bp)
ACCCCTGTTTTTGGTCCCAAGGCTCGCCTGGAATTCTCGGGTGCCAAGGA1228520.13396960133651176RNA PCR Primer, Index 1 (100% over 22bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGA1225870.13368061992510474RNA PCR Primer, Index 19 (100% over 37bp)
CTGTTTTTGGTCCCAAGGCTCGCTTGGAATTCTCGGGTGCCAAGGAACTC1225810.13367407694975214RNA PCR Primer, Index 1 (100% over 26bp)
CCCCTGTTTTTGGTCCCAAGGCTCGCTTTGGAATTCTCGGGTGCCAAGGA1213960.13238183931761127RNA PCR Primer, Index 1 (100% over 22bp)
CACGTCTGCCTGGGTGTCACATGGAATTCTCGGGTGCCAAGGAACTCCAG1202530.13113540251293873RNA PCR Primer, Index 1 (100% over 29bp)
GAGGGCACGTCTGCCTGGGTGTCTGGAATTCTCGGGTGCCAAGGAACTCC1188850.12964360413254325RNA PCR Primer, Index 1 (100% over 27bp)
CAAGGCTCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAA1178690.12853566030616762RNA PCR Primer, Index 19 (100% over 40bp)
GTGAACCATCGAGTCTTTGAACGCAATGGAATTCTCGGGTGCCAAGGAAC1141960.12453026889447708RNA PCR Primer, Index 1 (100% over 24bp)
CGTGAACCATCGAGTCTTTGAACGCTGGAATTCTCGGGTGCCAAGGAACT1127220.12292287794951876RNA PCR Primer, Index 1 (100% over 25bp)
ACGCTTTTATTAGATCAAAACCGGCCTGGAATTCTCGGGTGCCAAGGAAC1115780.1216753506489541RNA PCR Primer, Index 1 (100% over 24bp)
AACCCCTGTTTTTGGTCCCAAGGCTCGTGGAATTCTCGGGTGCCAAGGAA1096580.11958159853611831RNA PCR Primer, Index 1 (100% over 23bp)
CGAGGGCACGTCTGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG1080570.11783571461286306RNA PCR Primer, Index 6 (100% over 34bp)
GTTTTTGGTCCCAAGGCTCGCTGGAATTCTCGGGTGCCAAGGAACTCCAG1024090.11167659381427111RNA PCR Primer, Index 1 (100% over 29bp)
TTTTGGTCCCAAGGCTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT1019400.11116515124087528RNA PCR Primer, Index 1 (100% over 30bp)
ACCCCTGTTTTTGGTCCCAAGGCTGGAATTCTCGGGTGCCAAGGAACTCC976050.10643785154861322RNA PCR Primer, Index 1 (100% over 27bp)
CCGAGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATC955640.10421214943283309RNA PCR Primer, Index 19 (100% over 42bp)
AGGGCACGTCTGCCTGGGTGTCACATGGAATTCTCGGGTGCCAAGGAACT950520.10365381553607687RNA PCR Primer, Index 1 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph