FastQCFastQC Report
Tue 4 Jul 2023
SRR6051827.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6051827.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118382525
Total Bases5.9 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCATTTATTAGATAAAAGGTCGACGTGGAATTCTCGGGTGCCAAGGAA79384176.705733806573225RNA PCR Primer, Index 1 (100% over 23bp)
ACCCCTGTTTTTGGTCCCAAGGCTCGCTGGAATTCTCGGGTGCCAAGGAA71573876.045982715776674RNA PCR Primer, Index 1 (100% over 23bp)
CGAGGGCACGTCTGCCTGGGTGTCACATGGAATTCTCGGGTGCCAAGGAA63110705.3310824380540955RNA PCR Primer, Index 1 (100% over 23bp)
GAAGGTCACGGCGAGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC45248833.8222558608206745RNA PCR Primer, Index 1 (100% over 33bp)
ACCCCTGTTTTTGGTCCCAAGGCTCGCTTGGAATTCTCGGGTGCCAAGGA41420533.498871983005938RNA PCR Primer, Index 1 (100% over 22bp)
CCGAGGGCACGTCTGCCTGGGTGTCACATGGAATTCTCGGGTGCCAAGGA22647881.9131100641754346RNA PCR Primer, Index 1 (100% over 22bp)
CGAGGGCACGTCTGCCTGGGTGTCTGGAATTCTCGGGTGCCAAGGAACTC18839921.5914443453541811RNA PCR Primer, Index 1 (100% over 26bp)
CCCCTGTTTTTGGTCCCAAGGCTCGCTTGGAATTCTCGGGTGCCAAGGAA17376841.467855158521074RNA PCR Primer, Index 1 (100% over 23bp)
CCGAGGGCACGTCTGCCTGGGTGTCTGGAATTCTCGGGTGCCAAGGAACT16934691.4305058960349089RNA PCR Primer, Index 1 (100% over 25bp)
ACGCATTTATTAGATAAAAGGTCTGGAATTCTCGGGTGCCAAGGAACTCC8227360.6949809526363794RNA PCR Primer, Index 1 (100% over 27bp)
ACGCATTTATTAGATAAAAGGTCGACGCTGGAATTCTCGGGTGCCAAGGA8156790.6890197687538765RNA PCR Primer, Index 1 (100% over 22bp)
CCGAGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCCATC6931270.5854977328790715RNA PCR Primer, Index 20 (100% over 42bp)
AACCCCTGTTTTTGGTCCCAAGGCTCGCTGGAATTCTCGGGTGCCAAGGA4417290.37313699804933204RNA PCR Primer, Index 1 (100% over 22bp)
ACCCCTGTTTTTGGTCCCAAGGCTCGTGGAATTCTCGGGTGCCAAGGAAC3344320.28250115462565106RNA PCR Primer, Index 1 (100% over 24bp)
ACGCATTTATTAGATAAAAGGTCGACTGGAATTCTCGGGTGCCAAGGAAC3326850.2810254300624184RNA PCR Primer, Index 1 (100% over 24bp)
ACCCCTGTTTTTGGTCCCAAGGCTCTGGAATTCTCGGGTGCCAAGGAACT2740460.2314919368378061RNA PCR Primer, Index 1 (100% over 25bp)
AGGGAGAACTGCGAGGAGCCAACCCTTGGAATTCTCGGGTGCCAAGGAAC2737410.23123429746071053RNA PCR Primer, Index 1 (100% over 24bp)
ACGTCTGCCTGGGTGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC2710910.22899579139742118RNA PCR Primer, Index 1 (100% over 33bp)
CCGAGGGCACGTCTGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC2627450.2219457643769636RNA PCR Primer, Index 1 (100% over 33bp)
GAGGGCACGTCTGCCTGGGTGTCTGGAATTCTCGGGTGCCAAGGAACTCC2539700.21453335279003383RNA PCR Primer, Index 1 (100% over 27bp)
CGAGGGCACGTCTGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG2495450.21079547002397525RNA PCR Primer, Index 6 (100% over 34bp)
CACGTCTGCCTGGGTGTCACATGGAATTCTCGGGTGCCAAGGAACTCCAG2414960.20399632462645986RNA PCR Primer, Index 1 (100% over 29bp)
ACCCCTGTTTTTGGTCCCAAGGCTCGCCTGGAATTCTCGGGTGCCAAGGA2385920.20154325986880242RNA PCR Primer, Index 1 (100% over 22bp)
CCGAGGGCACGTCTGCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA2305190.19472384120882708RNA PCR Primer, Index 1 (100% over 32bp)
CGAGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCCATCT2258590.1907874494145145RNA PCR Primer, Index 20 (100% over 43bp)
CCGAGGGCACGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGC2246520.18976787325663141RNA PCR Primer, Index 20 (100% over 38bp)
GAAGGTCACGGCGAGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG2228100.18821190036282803RNA PCR Primer, Index 6 (100% over 34bp)
CGAGGGCACGTCTGCCTGGGTGTCACTGGAATTCTCGGGTGCCAAGGAAC2097110.17714692265602547RNA PCR Primer, Index 1 (100% over 24bp)
GGTCACGGCGAGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTG1781430.15048082476700003RNA PCR Primer, Index 19 (100% over 36bp)
CCGAGGGCACGTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG1700660.143658027229948RNA PCR Primer, Index 6 (100% over 34bp)
AAGGTCACGGCGAGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG1588520.13418534534552293RNA PCR Primer, Index 6 (100% over 34bp)
ATCCAAGTTGAAAGAGATAAATTGAACATGGAATTCTCGGGTGCCAAGGA1549580.13089600851139135RNA PCR Primer, Index 1 (100% over 22bp)
GAAGGTCACGGCGAGACGAGCTGGAATTCTCGGGTGCCAAGGAACTCCAG1538200.12993471798308068RNA PCR Primer, Index 1 (100% over 29bp)
TGGCCGAGGGCACGTCTGCCTGTGGAATTCTCGGGTGCCAAGGAACTCCA1505580.1271792437270619RNA PCR Primer, Index 1 (100% over 28bp)
AGGTCACGGCGAGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT1464490.12370829225005972RNA PCR Primer, Index 17 (100% over 35bp)
CCCCTGTTTTTGGTCCCAAGGCTCGCCTGGAATTCTCGGGTGCCAAGGAA1458850.1232318705822502RNA PCR Primer, Index 1 (100% over 23bp)
CCCCTGTTTTTGGTCCCAAGGCTCGCTTTGGAATTCTCGGGTGCCAAGGA1449390.12243276615361938RNA PCR Primer, Index 1 (100% over 22bp)
TCCAAGTTGAAAGAGATAAATTGAACATGGAATTCTCGGGTGCCAAGGAA1446980.12222918880975044RNA PCR Primer, Index 1 (100% over 23bp)
GAAGGTCACGGCGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTG1407090.11885960364504812RNA PCR Primer, Index 19 (100% over 36bp)
CCGAGGGCACGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCC1383980.11690745741400599RNA PCR Primer, Index 20 (100% over 39bp)
ACCGAGATCTTTGGAGATGATTCTGTACTGGAATTCTCGGGTGCCAAGGA1279600.10809027768245356RNA PCR Primer, Index 1 (100% over 22bp)
CCGAGGGCACGTCTGCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1268000.10711040333022126RNA PCR Primer, Index 1 (100% over 31bp)
CCCCTGTTTTTGGTCCCAAGGCTCGCTGGAATTCTCGGGTGCCAAGGAAC1242620.10496650582507848RNA PCR Primer, Index 1 (100% over 24bp)
AACCCCTGTTTTTGGTCCCAAGGCTCGTGGAATTCTCGGGTGCCAAGGAA1215570.10268154020198506RNA PCR Primer, Index 1 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)