FastQCFastQC Report
Tue 4 Jul 2023
SRR6051828.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6051828.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106939566
Total Bases5.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGGGCACGTCTGCCTGGGTGTCACATGGAATTCTCGGGTGCCAAGGAA105492079.864643550171131RNA PCR Primer, Index 1 (100% over 23bp)
ACCCCTGTTTTTGGTCCCAAGGCTCGCTGGAATTCTCGGGTGCCAAGGAA26235902.4533389260248164RNA PCR Primer, Index 1 (100% over 23bp)
CGAGGGCACGTCTGCCTGGGTGTCTGGAATTCTCGGGTGCCAAGGAACTC25493532.383919343753462RNA PCR Primer, Index 1 (100% over 26bp)
CCGAGGGCACGTCTGCCTGGGTGTCTGGAATTCTCGGGTGCCAAGGAACT21609912.0207590892972203RNA PCR Primer, Index 1 (100% over 25bp)
CCGAGGGCACGTCTGCCTGGGTGTCACATGGAATTCTCGGGTGCCAAGGA14477371.353789859218243RNA PCR Primer, Index 1 (100% over 22bp)
CGAGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCGATCT13802141.2906485893163246RNA PCR Primer, Index 21 (100% over 43bp)
CCGAGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCGATC13426091.2554838683373748RNA PCR Primer, Index 21 (100% over 42bp)
CCCCTGTTTTTGGTCCCAAGGCTCGCTTGGAATTCTCGGGTGCCAAGGAA13124701.2273006606366814RNA PCR Primer, Index 1 (100% over 23bp)
ACCCCTGTTTTTGGTCCCAAGGCTCGCTTGGAATTCTCGGGTGCCAAGGA12806831.1975763956251702RNA PCR Primer, Index 1 (100% over 22bp)
ACGTCTGCCTGGGTGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC11222741.049446937160751RNA PCR Primer, Index 1 (100% over 33bp)
GAAGGTCACGGCGAGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC11097121.0377001155961303RNA PCR Primer, Index 1 (100% over 33bp)
GCACGTCTGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCG7925330.741103624826755RNA PCR Primer, Index 21 (100% over 39bp)
CCCCTGTTTTTGGTCCCAAGGCTCGCTGGAATTCTCGGGTGCCAAGGAAC6564030.6138074283937154RNA PCR Primer, Index 1 (100% over 24bp)
GAGGGCACGTCTGCCTGGGTGTCTGGAATTCTCGGGTGCCAAGGAACTCC6059950.5666705249205892RNA PCR Primer, Index 1 (100% over 27bp)
ACCCCTGTTTTTGGTCCCAAGGCTCTGGAATTCTCGGGTGCCAAGGAACT5214570.4876183993490305RNA PCR Primer, Index 1 (100% over 25bp)
CCGAGGGCACGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCG4719270.44130252034125517RNA PCR Primer, Index 21 (100% over 39bp)
AACCCCTGTTTTTGGTCCCAAGGCTCGCTGGAATTCTCGGGTGCCAAGGA3793840.35476485849961276RNA PCR Primer, Index 1 (100% over 22bp)
AGGGCACGTCTGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTT3745520.35024641861740863RNA PCR Primer, Index 21 (100% over 36bp)
CGAGGGCACGTCTGCCTGGGTGTCACTGGAATTCTCGGGTGCCAAGGAAC3583650.3351098320335431RNA PCR Primer, Index 1 (100% over 24bp)
TTTTTGGTCCCAAGGCTCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT3195940.2988547756028858RNA PCR Primer, Index 1 (100% over 30bp)
GCACGTCTGCCTGGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG3181680.29752131217738437RNA PCR Primer, Index 6 (100% over 34bp)
CACGTCTGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCGA3160600.29555010537446913RNA PCR Primer, Index 21 (100% over 40bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTT3062130.2863421009208135RNA PCR Primer, Index 21 (100% over 37bp)
CGAGGGCACGTCTGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG2871580.26852362576448086RNA PCR Primer, Index 6 (100% over 34bp)
GGCACGTCTGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTC2771800.25919312221633667RNA PCR Primer, Index 21 (100% over 38bp)
TTTTTGGTCCCAAGGCTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG2770900.25910896253310023RNA PCR Primer, Index 1 (100% over 29bp)
CGAGGGCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCGATC2579660.24122596495295295RNA PCR Primer, Index 21 (100% over 42bp)
CCGAGGGCACGTCTGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC2559180.2393108646055287RNA PCR Primer, Index 1 (100% over 33bp)
TCTGCCTGGGTGTCACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC2100550.19642402513584167RNA PCR Primer, Index 1 (100% over 33bp)
GGCACGTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCG2072910.19383938775289214RNA PCR Primer, Index 21 (100% over 39bp)
CTGGGTGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCGAT1996230.18666898274114935RNA PCR Primer, Index 21 (100% over 41bp)
CTGGGTGTCACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTC1920050.17954533310898232RNA PCR Primer, Index 21 (100% over 38bp)
CCCCTGTTTTTGGTCCCAAGGCTCGCCTGGAATTCTCGGGTGCCAAGGAA1852320.17321184939164613RNA PCR Primer, Index 1 (100% over 23bp)
GGGCACGTCTGCCTGGGTGTCACATGGAATTCTCGGGTGCCAAGGAACTC1767900.16531767110407014RNA PCR Primer, Index 1 (100% over 26bp)
CGTGAACCATCGAGTCTTTGAACGCAATGGAATTCTCGGGTGCCAAGGAA1580060.1477526101050382RNA PCR Primer, Index 1 (100% over 23bp)
CACGTCTGCCTGGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT1527630.1428498410027211RNA PCR Primer, Index 17 (100% over 35bp)
AGGGAGAACTGCGAGGAGCCAACCCTGGAATTCTCGGGTGCCAAGGAACT1519710.14210923579024065RNA PCR Primer, Index 1 (100% over 25bp)
ACGTCTGCCTGGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTT1516490.14180813114577256RNA PCR Primer, Index 21 (100% over 36bp)
ATGTACACGGAGTCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1482730.13865120791681537RNA PCR Primer, Index 1 (100% over 32bp)
AACCCCTGTTTTTGGTCCCAAGGCTCTGGAATTCTCGGGTGCCAAGGAAC1418160.13261321819839816RNA PCR Primer, Index 1 (100% over 24bp)
ACGTCTGCCTGGGTGTCACATGGAATTCTCGGGTGCCAAGGAACTCCAGT1412580.13209142816233238RNA PCR Primer, Index 1 (100% over 30bp)
GAGGGCACGTCTGCCTGGGTGTCACATGGAATTCTCGGGTGCCAAGGAAC1342550.12554286969894754RNA PCR Primer, Index 1 (100% over 24bp)
GAGGGCACGTCTGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT1287620.1204063236987515RNA PCR Primer, Index 17 (100% over 35bp)
CCGAGGGCACGTCTGCCTGGGTGTGGAATTCTCGGGTGCCAAGGAACTCC1280380.11972930580249408RNA PCR Primer, Index 1 (100% over 27bp)
CCCCTGTTTTTGGTCCCAAGGCTCTGGAATTCTCGGGTGCCAAGGAACTC1268790.11864551610392735RNA PCR Primer, Index 1 (100% over 26bp)
ACGTCTGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCGAT1247040.11661165709238057RNA PCR Primer, Index 21 (100% over 41bp)
ACCCCTGTTTTTGGTCCCAAGGCTCGTGGAATTCTCGGGTGCCAAGGAAC1219350.11402234417147346RNA PCR Primer, Index 1 (100% over 24bp)
GGCACGTCTGCCTGGGTGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGT1179970.11033989047608442RNA PCR Primer, Index 1 (100% over 30bp)
CGGCGAGACGAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTC1170820.10948426702984748RNA PCR Primer, Index 21 (100% over 38bp)
CCGAGGGCACGTCTGCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1155810.10808067053498234RNA PCR Primer, Index 1 (100% over 32bp)
ACGCATTTATTAGATAAAAGGTCGACGTGGAATTCTCGGGTGCCAAGGAA1151990.10772345943502333RNA PCR Primer, Index 1 (100% over 23bp)
CACGTCTGCCTGGGTGTCACATGGAATTCTCGGGTGCCAAGGAACTCCAG1130040.10567089827164625RNA PCR Primer, Index 1 (100% over 29bp)
TCTGCCTGGGTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTC1124760.10517716146332594RNA PCR Primer, Index 21 (100% over 38bp)
CCCCTGTTTTTGGTCCCAAGGCTCGCTTTGGAATTCTCGGGTGCCAAGGA1110220.10381751502526201RNA PCR Primer, Index 1 (100% over 22bp)
ACCCCTGTTTTTGGTCCCAAGGCTCGCCTGGAATTCTCGGGTGCCAAGGA1099650.10282910630102987RNA PCR Primer, Index 1 (100% over 22bp)
ATGTACACGGAGTCGACCCGCAACGCGTGGAATTCTCGGGTGCCAAGGAA1089820.10190989553856988RNA PCR Primer, Index 1 (100% over 23bp)
CGAGGGCACGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCGA1071560.10020238907646212RNA PCR Primer, Index 21 (100% over 40bp)

[FAIL]Adapter Content

Adapter graph