FastQCFastQC Report
Thu 28 Sept 2023
SRR6053327_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6053327_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30518282
Total Bases967.1 Mbp
Sequences flagged as poor quality0
Sequence length25-44
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGAGTCGACCCGCAACGCGA21823977.151113552197991No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT21270336.969700981202022No Hit
TCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG13639004.469124441539664No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT5281471.7305921742252726No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC4699211.5398016179285583No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC3628461.1889463502565445No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT3625621.1880157605202022No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC3456471.1325899668926318No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG2743510.8989726223776293No Hit
TCGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC2698720.8842961736836956No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA2691340.8818779510589752No Hit
TGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG1828840.5992604695113571No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC1616020.5295252203253119No Hit
TACACGGAGTCGACCCGCAACGCGA1540550.5047957810993424No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG1427960.46790314081244805No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC1386400.45428507410738256No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGA1313190.43029617460117836No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT1310270.4293393710694462No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT1265390.4146334318556988No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC1142810.3744673438694878No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC1105360.36219601090257963No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG844990.2768799370816483No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG793210.25991305801552No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCC730320.23930573811461603No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC722900.23687440859220055No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC704990.23100579514928132No Hit
ACCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT653090.21399959539006816No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG582460.190856090785189No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCT556930.18249061333137953No Hit
TCGACTGCATAATTTGTGGTAGTAGGGGA549570.18007894415550652No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGA541530.17744445771881917No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGG536510.17579954205810142No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGA521120.1707566631699648No Hit
TCGACTGCATAATTTGTGGTAGTAGGGG510390.16724073786329124No Hit
ACGCACGCGCGCGCGCGCGCGCGGAC505230.16554994806064116No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC497270.1629416754193437No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGG488110.15994019584719743No Hit
TTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG482990.15826251294224228No Hit
TCGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGA472020.15466794624939897No Hit
ACGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG460710.15096197092614846No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGA453810.1487010310737675No Hit
TGCCCGGCTAGCTCAGTCGGTAGAGCATGGGA445530.14598790325091038No Hit
ACGGGCCCCTCTCTCGGGGCGAACCC433030.14189199772123479No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGTTA418450.1371145335114211No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC405890.13299896763520305No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC388090.12716639816094497No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGAC384850.12610473944765305No Hit
TCGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGAC382090.1252003635067007No Hit
ACGGGCCCCTCTCTCGGGGCGAACCCATTCC374230.12262485810964063No Hit
CCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG372180.12195312960277384No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCC365400.1197315104434778No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGC342500.11222781151311204No Hit
TCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGC341880.11202465459884013No Hit
TGTACACGGAGTCGACCCGCAACGCGA330410.10826625168480979No Hit
TTCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG321080.1052090677974599No Hit
TTCTCGCTTCTGGCGCCAAGCGCCCGGCCG307020.10060199325768075No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)