FastQCFastQC Report
Thu 28 Sept 2023
SRR6053328_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6053328_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30360609
Total Bases975.6 Mbp
Sequences flagged as poor quality0
Sequence length25-44
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT385465512.696237417371965No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT13294774.37895366328126No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT11240273.7022544574122342No Hit
TCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG5140491.6931445610988898No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC4215981.3886348590701854No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC3527601.1619002767698106No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT3491071.1498682388090435No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT3292931.084606043310923No Hit
TGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG2794190.9203339761728758No Hit
ATGTACACGGAGTCGACCCGCAACGCGA2713860.8938753501288463No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC2658970.8757960026427665No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC2298040.7569149881018526No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA2115840.6969030166687368No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC2040500.6720879676688962No Hit
TCGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC2025130.6670254868734682No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC1487250.4898617152244871No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG1406580.4632911019670258No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC1402390.4619110242485584No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG1402040.46179574329355516No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGA1280790.42185912673886083No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGA1169130.3850812083512554No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC1132020.3728581333793403No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT1128120.3715735741664471No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT1122320.3696632040549648No Hit
ACCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT1095580.3608557390927171No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG1016120.3346836685654099No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG979740.3227010367282158No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC930680.30654194057833295No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC894230.29453625255013827No Hit
TGCCCGGCTAGCTCAGTCGGTAGAGCATGGGA794770.2617766988797886No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGG771460.2540989872765727No Hit
GCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT720980.23747217982353383No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT699030.23024241707404486No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCC686430.22609230269392816No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGA684200.22535779832347896No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC674820.22226826872939207No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGA658050.21674466411395107No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT645850.21272629939669527No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC636220.20955442626332033No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC590430.19447238360732488No Hit
TTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG585030.19269376315870343No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGA573260.18881702932902303No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGC545950.17982182109719866No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG545190.17957149673776307No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGTTA541920.17849444324387564No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCT518380.1707409755845148No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGG512490.16880096179888882No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGA512000.16863956846188427No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTT494810.16297762670043936No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC485240.1598255160164936No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGAC470740.1550495907377879No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG468370.15426897398533737No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTTA465610.15335990131159755No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGG456380.1503197778410835No Hit
TGCCCGGCTAGCTCAGTCGGTAGAGCATGAGA453310.1493085991786265No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGC439660.14481264193350007No Hit
TCGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGA411030.13538265981423495No Hit
TCGCCGCTGGTGTAGTGGTATCATGCAAGATT394040.12978659288422048No Hit
TAACGATGCCGACCGGCGATGCGGCGGCGTTAT393500.12960873083935834No Hit
TTGGTCTAGGGGTATGATTCTCGCTT385370.1269309189417116No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC373590.12305089137045967No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCT369430.12168069487670685No Hit
TGCCCGGATAGCTCAGTCGGTAGAGCATCAGA362830.11950682543950288No Hit
TTCCCTGGTGGTCTAGTGGTTAGGATTCGGCG357200.11765244893473645No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCC353800.11653257680041926No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCT350230.11535671105938619No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT345860.11391734599263142No Hit
TTCTCGCTTCTGGCGCCAAGCGCCCGGCCG339380.11178300145428571No Hit
ACTCGACTGCATAATTTGTGGTAGTGGGGGA334960.11032716767967335No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCT330930.10899978982635032No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTTAT330430.10883510274777428No Hit
TCGACTGCATAATTTGTGGTAGTAGGGGA317350.10452688877222456No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCG312850.10304470506504004No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT305770.10071273603240304No Hit
TGCCCGGCTAGCTCAGTCGGTAGAGCATGGG305650.10067321113354478No Hit
TCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGC304700.10036030568425028No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)