FastQCFastQC Report
Thu 28 Sept 2023
SRR6053329_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6053329_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29514081
Total Bases940.1 Mbp
Sequences flagged as poor quality0
Sequence length25-43
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT378884612.83741818015611No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT25972588.800063942360259No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT12393454.199165137481327No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT5960252.019459796156282No Hit
TCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG4454121.5091508354944205No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT3383081.1462596446760447No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC3344191.1330828833870856No Hit
TGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG2334800.7910800271910888No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA1938050.6566526669083819No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC1853390.6279680536215917No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT1744110.5909416593388085No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC1647920.5583504361867138No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC1495680.5067682778264382No Hit
ACCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT1209690.40986876738598094No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG1157660.3922398939001353No Hit
TCGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC1008550.341718246283867No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC861370.29185052382284915No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG859350.29116610474844196No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCT807310.2735338430493567No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT767840.26016056539249854No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC763150.25857149338310753No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCT752670.2550206459079651No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT727940.24664159456633597No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC721040.24430372743098455No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC717300.2430365356793593No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC704570.23872334022529787No Hit
GCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT665850.2256041785614128No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGG658060.2229647604477334No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC624810.21169895142593123No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC624100.21145838828591684No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCT614750.20829040890685363No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC573160.19419883004319194No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGA570010.19313154287270543No Hit
TTCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT520720.17643103981452107No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC512110.17351378821519126No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGA499920.1693835562760704No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG490880.16632061150743607No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT480650.1628544693632846No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGA470510.1594188211382899No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT470190.15931039831462143No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC458050.15519710744169876No Hit
TTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC450860.15276098212239778No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTT449530.1523103497615257No Hit
ATGTACACGGAGTCGACCCGCAACGCGA443860.15038923285464997No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGC442270.14985050694954724No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCT413990.14026863990784602No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTTA409940.13889641354579194No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT397800.13478312267286927No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGTTA382640.12964659140157542No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCC349020.11825541848990656No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGAC342350.11599548025906685No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGC340320.11530767297141997No Hit
TGCCCGGCTAGCTCAGTCGGTAGAGCATGGGA337740.11443351395559294No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC336300.11394561124908481No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGG335500.11367455418991362No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGG330940.1121295289526379No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG326710.11069631475227029No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC316270.10715902013008638No Hit
TTCTCGCTTCTGGCGCCAAGCGCCCGGCCG309030.1047059537445872No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCC308660.10458058985472053No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT303840.10294747107321418No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC303640.10287970680842137No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)