FastQCFastQC Report
Thu 28 Sept 2023
SRR6053474_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6053474_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8282319
Total Bases410.5 Mbp
Sequences flagged as poor quality0
Sequence length37-50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTTGGAATTCTCGG417640.5042549073514314No Hit
AGCAGGGTCGGGCCTGGTTAGTACTTGGATGGAATTCTCGGGTGCCAAGG346300.4181196111861908Illumina Small RNA Adapter 2 (100% over 21bp)
AGCGGGAAACTTGCCTCAAGATGAGATCTCACTTGGAATTCTCGGGTGCC335320.4048624545854851No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC259130.3128713105592769No Hit
TGAGATTTGAGAGGGGCTGCTCCTTGGAATTCTCGGGTGCCAAGGAACTC246120.2971631495961457RNA PCR Primer, Index 1 (100% over 26bp)
CGGGGCTCAAACCATACACCGAAGCTACGGGTGGAATTCTCGGGTGCCA243720.2942654104484505No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGTGGAATTCTCGGGTGCCAAGG242770.2931183887024878Illumina Small RNA Adapter 2 (100% over 21bp)
AGCAGGGTCGGGCCTGGTTAGTACTTGGTGGAATTCTCGGGTGCCAAGG221490.26742510159292343Illumina Small RNA Adapter 2 (100% over 21bp)
TTGAGAGAACTCGGGTGAAGGAACTTGGAATTCTCGGGTGCCAAGGAACT207400.25041295801332936RNA PCR Primer, Index 1 (100% over 25bp)
AAGCAGGGTCGGGCCTGGTTAGTACTTGGATGGAATTCTCGGGTGCCA205650.2483000232181349No Hit
CTCAGATTGAACGCTGGCGGCAGGCCTGGAATTCTCGGGTGCCAAGGAAC179170.21632830128856423RNA PCR Primer, Index 1 (100% over 24bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTGGAATTCTCGGG173160.20907187950621078No Hit
GGTGGTTGGGTAGAGAATACCAAGGCGCTGGAATTCTCGGGTGCCAAGG166690.20126005772054903Illumina Small RNA Adapter 2 (100% over 21bp)
AATACCAAGGCGCTTGAGAGAACTCGGGTGAAGGAACTTGGAATTCTCGG162260.19591131421042826No Hit
TGGCGGCGCTGTCTCCACCCGAGACTCAGTGGAATTCTCGGGTGCCAAGG155870.1881960837296897Illumina Small RNA Adapter 2 (100% over 21bp)
TGTCAACTAGCCGTTGGGAGCCTGGAATTCTCGGGTGCCAAGGAACTCC154350.1863608489361494RNA PCR Primer, Index 1 (100% over 27bp)
CAGATAACCAGGAACCGTACCCCAAACCGACTGGAATTCTCGGGTGCCA153870.18578130110661037No Hit
AGCGGAACGCTCTGGAAAGTGCGGCCTGGAATTCTCGGGTGCCAAGGAAC153560.18540700980003305RNA PCR Primer, Index 1 (100% over 24bp)
GGCGGCGCTGTCTCCACCCGAGACTCAGTGGAATTCTCGGGTGCCAAGG153000.18473087066557084Illumina Small RNA Adapter 2 (100% over 21bp)
TACCAAGGCGCTTGAGAGAACTCGGGTGAAGGAACTTGGAATTCTCGGGT151790.18326992717860785No Hit
ATACCAAGGCGCTTGAGAGAACTCGGGTGAAGGAACTTGGAATTCTCGGG141200.17048365318940265No Hit
TACTACTGACTGACCGATAGTGAACTTGGAATTCTCGGGTGCCAAGGAAC140570.16972299666313265RNA PCR Primer, Index 1 (100% over 24bp)
ATACTACTGACTGACCGATAGTGAACTTGGAATTCTCGGGTGCCAAGGA140450.16957810970574788RNA PCR Primer, Index 1 (100% over 22bp)
ACCAAGGCGCTTGAGAGAACTCGGGTGAAGGAACTTGGAATTCTCGGGTG135960.16415692271693472No Hit
GTCAACTAGCCGTTGGGAGCCTGGAATTCTCGGGTGCCAAGGAACTCC131060.1582407052903903RNA PCR Primer, Index 1 (100% over 27bp)
TGGCGGCGCTGTCTCCACCCGAGACTCAGTTGGAATTCTCGGGTGCCA128850.1555723704918876No Hit
TCCGGAGATGTCTGAATGGGGGAACCCAGCCATCATGGAATTCTCGGGTG128280.15488415744431No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCGCTCTGGAATTCTCGGGTGCC124420.15022362698176683No Hit
CAGATAACCAGGAACCGTACCCCAAACCGACATGGAATTCTCGGGTGCC124040.14976481828338173No Hit
GGCGGCGCTGTCTCCACCCGAGACTCAGTTGGAATTCTCGGGTGCCAAGG116470.14062486605502636Illumina Small RNA Adapter 2 (100% over 21bp)
TGCCTCAAGATGAGATCTCACTGGAACCTGGAATTCTCGGGTGCCAAGG112480.13580737472198304Illumina Small RNA Adapter 2 (100% over 21bp)
GCAGGGTCGGGCCTGGTTAGTACTTGGATGGAATTCTCGGGTGCCAAGG111790.13497427471702067Illumina Small RNA Adapter 2 (100% over 21bp)
AGATCCTGAAACCGTATGCGTACAAGTGGAATTCTCGGGTGCCAAGGAAC108510.13101403121517052RNA PCR Primer, Index 1 (100% over 24bp)
AAGTTAGCGGATGACCTGTGGATCGGTGGAATTCTCGGGTGCCAAGGAAC107720.1300601920790542RNA PCR Primer, Index 1 (100% over 24bp)
CGGAACGCTCTGGAAAGTGCGGCCTGGAATTCTCGGGTGCCAAGGAACTC106040.12803177467566754RNA PCR Primer, Index 1 (100% over 26bp)
TCTTGACGACCATAGAGCGTTGGAACCACCTTGGAATTCTCGGGTGCCA105870.1278265181527058No Hit
AGAGAACTCGGGTGAAGGAACTTGGAATTCTCGGGTGCCAAGGAACTCC105110.12690890075593564RNA PCR Primer, Index 1 (100% over 27bp)
CGGCGCTGTCTCCACCCGAGACTCAGTGGAATTCTCGGGTGCCAAGGAAC104050.12562906596570356RNA PCR Primer, Index 1 (100% over 24bp)
TGGCTCAGATTGAACGCTGGCGGCAGGCCTGGAATTCTCGGGTGCCAAGG102470.12372138769347087Illumina Small RNA Adapter 2 (100% over 21bp)
TACATGCCGACGGGCGCTGACCCCCCTTGGAATTCTCGGGTGCCAAGGA102180.12337124421312436RNA PCR Primer, Index 1 (100% over 22bp)
CCCCATGTGAGAGTAGGTCATCGTCAAGATTGGAATTCTCGGGTGCCA101270.12227251811962325No Hit
CTTGCCTCAAGATGAGATCTCACTTGGAATTCTCGGGTGCCAAGGAACTC100120.12088401811135265RNA PCR Primer, Index 1 (100% over 26bp)
AGGCGCTTGAGAGAACTCGGGTGAAGGAACTTGGAATTCTCGGGTGCCA99890.12060631810969852No Hit
CGGCGCTGTCTCCACCCGAGACTCAGTTGGAATTCTCGGGTGCCAAGGA99160.11972492245227453RNA PCR Primer, Index 1 (100% over 22bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGGAATTCTCG98440.11885560070796597No Hit
TCATGGCTCAGATTGAACGCTGGCGGCAGGCCTGGAATTCTCGGGTGCC97210.11737050939477217No Hit
AACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCTGGAATTC96620.11665814852096375No Hit
AACACATGCAAGTCGAGCGGATGAGAGGAGCTTGGAATTCTCGGGTGCC95050.11476254416184646No Hit
AGGCCGAGAGCTGATGACGAGTTACCTTGGAATTCTCGGGTGCCAAGGA94220.11376040937326852RNA PCR Primer, Index 1 (100% over 22bp)
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCC93440.11281864415026757No Hit
AAAAGTTAGCGGATGACCTGTGGATCGGTGGAATTCTCGGGTGCCAAGG93220.11255301806172885Illumina Small RNA Adapter 2 (100% over 21bp)
AAGCAGGGTCGGGCCTGGTTAGTACTTGGTGGAATTCTCGGGTGCCAAGG91890.11094718761738107Illumina Small RNA Adapter 2 (100% over 21bp)
TTGAGATTTGAGAGGGGCTGCTCCTTGGAATTCTCGGGTGCCAAGGAACT90050.10872558760414808RNA PCR Primer, Index 1 (100% over 25bp)
AAATCAGTTATGGTTCCTTTGGTCGCTCGCTTGGAATTCTCGGGTGCCA89110.10759063977130076No Hit
AAATCCGGGATCTTAAGGCCGAGAGCTGATGACGAGTTACCTTGGAATTC88950.10739745716145442No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCA88590.10696279628930012No Hit
AGTTAGCGGATGACCTGTGGATCGGTGGAATTCTCGGGTGCCAAGGAACT88310.10662472672206902RNA PCR Primer, Index 1 (100% over 25bp)
GCGGCGCTGTCTCCACCCGAGACTCAGTGGAATTCTCGGGTGCCAAGGA87180.10526037454002919RNA PCR Primer, Index 1 (100% over 22bp)
AAAGTTAGCGGATGACCTGTGGATCGGTGGAATTCTCGGGTGCCAAGGA86460.10439105279572063RNA PCR Primer, Index 1 (100% over 22bp)
TAGCGGATGACCTGTGGATCGGTGGAATTCTCGGGTGCCAAGGAACTCC86030.10387187453175856RNA PCR Primer, Index 1 (100% over 27bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC85310.10300255278744999No Hit
CTCAGATTGAACGCTGGCGGCAGGCCTTGGAATTCTCGGGTGCCAAGGA85210.10288181365629603RNA PCR Primer, Index 1 (100% over 22bp)
TGAGATTAGCGGAACGCTCTGGAAAGTGCGGCCATGGAATTCTCGGGTGC84620.10216945278248761No Hit
ACTACTGACTGACCGATAGTGAACTTGGAATTCTCGGGTGCCAAGGAACT84620.10216945278248761RNA PCR Primer, Index 1 (100% over 25bp)
CTATGTTCGGGAAAGATAACCGCTTGGAATTCTCGGGTGCCAAGGAACTC83920.10132427886440983RNA PCR Primer, Index 1 (100% over 26bp)
GCGGCGCTGTCTCCACCCGAGACTCAGTTGGAATTCTCGGGTGCCAAGG83860.10125183538571744Illumina Small RNA Adapter 2 (100% over 21bp)
AAATCAGTTATGGTTCCTTTGGTCGCTCGCTGGAATTCTCGGGTGCCA82900.10009273972663936No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)