FastQCFastQC Report
Thu 28 Sept 2023
SRR6053475_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6053475_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5924856
Total Bases293.4 Mbp
Sequences flagged as poor quality0
Sequence length35-50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTGGAATTCTCGGG494850.8352101721965901No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTTGGAATTCTCGG432630.7301949617003349No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTTGGAATTCTCGGGTG362630.6120486303802152No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCC335980.5670686342419123No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCA282610.47699049563398666No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC263660.4450065959408971No Hit
CGGCGCTGTCTCCACCCGAGACTCAGTGGAATTCTCGGGTGCCAAGGAAC233660.39437245394656006RNA PCR Primer, Index 1 (100% over 24bp)
ACCACCCTGAACGCGCCCGATCTCGTGGAATTCTCGGGTGCCAAGGAACT206520.3485654334890165RNA PCR Primer, Index 1 (100% over 25bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGC180110.30399051048666836No Hit
CCACCCTGAACGCGCCCGATCTCGTGGAATTCTCGGGTGCCAAGGAACTC176630.2981169500153253RNA PCR Primer, Index 1 (100% over 26bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCTGGAATTCTCGGGT175340.29593968190956876No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGC150510.25403149038558914RNA PCR Primer, Index 2 (100% over 50bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGGAATTCTCG146940.24800602748826303No Hit
TCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTGGAATTCTCGGGT137880.2327145166059732No Hit
TGGCGGCGCTGTCTCCACCCGAGACTCAGTGGAATTCTCGGGTGCCAAGG135480.22866378524642625Illumina Small RNA Adapter 2 (100% over 21bp)
GGCGGCGCTGTCTCCACCCGAGACTCAGTGGAATTCTCGGGTGCCAAGG132940.22437676122423902Illumina Small RNA Adapter 2 (100% over 21bp)
TCTGATCTCGGAAGCTAAGCAGGGTCGGGCCTGGTGGAATTCTCGGGTGC129730.21895890803084495No Hit
GTCTACGGCCATACCACCCTGAACGTGGAATTCTCGGGTGCCAAGGAACT126510.21352417679011945RNA PCR Primer, Index 1 (100% over 25bp)
CGGAACGCTCTGGAAAGTGCGGCCTGGAATTCTCGGGTGCCAAGGAACTC120700.2037180312905495RNA PCR Primer, Index 1 (100% over 26bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTTGGAATTCTC116200.19612290999139895No Hit
TACCACCCTGAACGCGCCCGATCTCGTGGAATTCTCGGGTGCCAAGGAAC112050.189118520348849RNA PCR Primer, Index 1 (100% over 24bp)
TCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA105410.17791149692076905No Hit
CGGCGCTGTCTCCACCCGAGACTCAGTTGGAATTCTCGGGTGCCAAGGA105400.1778946188734376RNA PCR Primer, Index 1 (100% over 22bp)
TCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCA96890.16353140059437732No Hit
AGCGGAACGCTCTGGAAAGTGCGGCCTGGAATTCTCGGGTGCCAAGGAAC96660.16314320550575406RNA PCR Primer, Index 1 (100% over 24bp)
CTCAGATTGAACGCTGGCGGCAGGCCTGGAATTCTCGGGTGCCAAGGAAC93740.15821481568497192RNA PCR Primer, Index 1 (100% over 24bp)
ACCACCCTGAACGCGCCCGATCTCGTTGGAATTCTCGGGTGCCAAGGAAC93370.15759032793370842RNA PCR Primer, Index 1 (100% over 24bp)
GCGGCGCTGTCTCCACCCGAGACTCAGTGGAATTCTCGGGTGCCAAGGA92650.15637510852584435RNA PCR Primer, Index 1 (100% over 22bp)
ATGGCGGCGCTGTCTCCACCCGAGACTCAGTGGAATTCTCGGGTGCCA88970.15016398710787232No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATGGAATTC88330.1490837920786598No Hit
TGAGATTAGCGGAACGCTCTGGAAAGTGCGGCCATGGAATTCTCGGGTGC83320.14062789036560552No Hit
AGAGAACTCGGGTGAAGGAACTTGGAATTCTCGGGTGCCAAGGAACTCC82560.13934515876841563RNA PCR Primer, Index 1 (100% over 27bp)
ACCACCCTGAACGCGCCCGATCTTGGAATTCTCGGGTGCCAAGGAACTCC82380.13904135391644962RNA PCR Primer, Index 1 (100% over 27bp)
AGATTTGAGAGGGGCTGCTCCTTGGAATTCTCGGGTGCCAAGGAACTCC75940.1281718914349986RNA PCR Primer, Index 1 (100% over 27bp)
CCACCCTGAACGCGCCCGATCTCGTTGGAATTCTCGGGTGCCAAGGAACT74590.12589335504525342RNA PCR Primer, Index 1 (100% over 25bp)
TCTGATCTCGGAAGCTAAGCAGGGTCGGGCCTGGTTGGAATTCTCGGGTG73690.1243743307854233No Hit
CTCAGATTGAACGCTGGCGGCAGGCCTTGGAATTCTCGGGTGCCAAGGA72780.12283842847826175RNA PCR Primer, Index 1 (100% over 22bp)
TGGCGGCGCTGTCTCCACCCGAGACTCAGTTGGAATTCTCGGGTGCCA72020.12155569688107187No Hit
AACAGACTTTGATCCGGAGATTGGAATTCTCGGGTGCCAAGGAACTCC71840.12125189202910586RNA PCR Primer, Index 1 (100% over 27bp)
AAAACACAGCACTCTGCAAACACGAAAGTGGACGTGGAATTCTCGGGTGC70740.11939530682264683No Hit
CTCGGAAGCTAAGCAGGGTCGGGCCTGGTTGGAATTCTCGGGTGCCAAGG69960.11807881913079407Illumina Small RNA Adapter 2 (100% over 21bp)
TGGCGGCGCTGTCTCCACCCGAGTGGAATTCTCGGGTGCCAAGGAACTCC69300.11696486800691865RNA PCR Primer, Index 1 (100% over 27bp)
CGCCCGATCTCGTCTGATCTCGTGGAATTCTCGGGTGCCAAGGAACTCC68390.1154289656997571RNA PCR Primer, Index 1 (100% over 27bp)
GGCGGCGCTGTCTCCACCCGAGTGGAATTCTCGGGTGCCAAGGAACTCC68290.11526018522644264RNA PCR Primer, Index 1 (100% over 27bp)
AAACAGACTTTGATCCGGAGATGGAATTCTCGGGTGCCAAGGAACTCC67970.11472008771183638RNA PCR Primer, Index 1 (100% over 27bp)
CGCGCCCGATCTCGTCTGATCTCGTGGAATTCTCGGGTGCCAAGGAACTC67730.11431501457588168RNA PCR Primer, Index 1 (100% over 26bp)
CTGATCTCGGAAGCTAAGCAGGGTCGGGCCTGGTTGGAATTCTCGGGTGC66510.11225589280144531No Hit
CGGCGCTGTCTCCACCCGAGACTTGGAATTCTCGGGTGCCAAGGAACTCC65300.11021364907434036RNA PCR Primer, Index 1 (100% over 27bp)
AGATCCTGAAACCGTATGCGTACAAGTGGAATTCTCGGGTGCCAAGGAAC65060.10980857593838568RNA PCR Primer, Index 1 (100% over 24bp)
CGGCGCTGTCTCCACCCGAGACTCAGTGTGGAATTCTCGGGTGCCAAGG64670.10915033209245928Illumina Small RNA Adapter 2 (100% over 21bp)
TTCTTGACGACCATAGAGCGTTGGAACCTGGAATTCTCGGGTGCCAAGG64570.10898155161914484Illumina Small RNA Adapter 2 (100% over 21bp)
TGAGATTTGAGAGGGGCTGCTCCTTGGAATTCTCGGGTGCCAAGGAACTC64470.10881277114583039RNA PCR Primer, Index 1 (100% over 26bp)
TGGCGGCGCTGTCTCCACCCGAGACTTGGAATTCTCGGGTGCCAAGGAAC64180.10832330777321844RNA PCR Primer, Index 1 (100% over 24bp)
GAGAGTAGGTCATCGTCAAGATTTGGAATTCTCGGGTGCCAAGGAACTCC63820.10771569806928641RNA PCR Primer, Index 1 (100% over 27bp)
CCACCCTGAACGCGCCCGATCTTGGAATTCTCGGGTGCCAAGGAACTCC63010.10634857623543931RNA PCR Primer, Index 1 (100% over 27bp)
ACGATGGCGGCGCTGTCTCCACCCGAGACTCAGTGGAATTCTCGGGTGCC62330.105200869016901No Hit
TCCGGATCGCAGTCTGCAACTCGACTGCGTGTGGAATTCTCGGGTGCCA61690.10412067398768848No Hit
TCTTGACGACCATAGAGCGTTGGAACCACCTTGGAATTCTCGGGTGCCA61590.10395189351437402No Hit
TCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC60150.10152145469864585No Hit
AACAGACTTTGATCCGGAGATGTGGAATTCTCGGGTGCCAAGGAACTCC60140.1015045766513144RNA PCR Primer, Index 1 (100% over 27bp)
AAGATACCAGGCCGCTGCGACTGTGGAATTCTCGGGTGCCAAGGAACTCC59960.10120077179934837RNA PCR Primer, Index 1 (100% over 27bp)
GGCGGCGCTGTCTCCACCCGAGACTTGGAATTCTCGGGTGCCAAGGAACT59730.1008125767107251RNA PCR Primer, Index 1 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)