FastQCFastQC Report
Thu 28 Sept 2023
SRR6053476_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6053476_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5040198
Total Bases250.2 Mbp
Sequences flagged as poor quality0
Sequence length37-50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATCCGGGATCTTAAGGCCGAGAGCTGATGACGAGTTACCTTGGAATTC495550.9831955014465701No Hit
AGCGGGAAACTTGCCTCAAGATGAGATCTCACTTGGAATTCTCGGGTGCC324840.6444984899402761No Hit
AACTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTGG240890.4779375730874065No Hit
GGTGGTTGGGTAGAGAATACCAAGGCGCTGGAATTCTCGGGTGCCAAGG192060.38105645849627334Illumina Small RNA Adapter 2 (100% over 21bp)
CAGATAACCAGGAACCGTACCCCAAACCGACATGGAATTCTCGGGTGCC183340.36375555087319983No Hit
CCCCATGTGAGAGTAGGTCATCGTCAAGATTGGAATTCTCGGGTGCCA175870.3489347045493054No Hit
ATACTACTGACTGACCGATAGTGAACTTGGAATTCTCGGGTGCCAAGGA169850.33699072933245877RNA PCR Primer, Index 1 (100% over 22bp)
CAGATAACCAGGAACCGTACCCCAAACCGACTGGAATTCTCGGGTGCCA164330.3260387786352838No Hit
TTGAGAGAACTCGGGTGAAGGAACTTGGAATTCTCGGGTGCCAAGGAACT157590.31266628811011RNA PCR Primer, Index 1 (100% over 25bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTTGGAATTCTCGG148060.29375830076516835No Hit
TCTGAATGGGGGAACCCAGCCATCACAAGATGGTTATCTTGTACTTGGA139660.2770922888346847No Hit
TACTACTGACTGACCGATAGTGAACTTGGAATTCTCGGGTGCCAAGGAAC131980.26185479221252816RNA PCR Primer, Index 1 (100% over 24bp)
CTCAGATTGAACGCTGGCGGCAGGCCTGGAATTCTCGGGTGCCAAGGAAC129130.2562002524503998RNA PCR Primer, Index 1 (100% over 24bp)
TTCTGTGACTGCGTACCTTTTGTATATGGAATTCTCGGGTGCCAAGGAAC126990.2519543875062051RNA PCR Primer, Index 1 (100% over 24bp)
CGGGGCTCAAACCATACACCGAAGCTACGGGTGGAATTCTCGGGTGCCA125510.24901799492797702No Hit
AATACCAAGGCGCTTGAGAGAACTCGGGTGAAGGAACTTGGAATTCTCGG123830.2456847925418803No Hit
CAGATAACCAGGAACCGTACCCCAAACCGACACATGGAATTCTCGGGTGC121430.2409230748474564No Hit
TCCTCCAAGGCTAAATACTACTGACTGACCGATAGTGAACTTGGAATTCT117200.23253054741103424No Hit
AGCGGGAAACTTGCCTCAAGATGAGATCTCACTGGAACCTGGAATTCTCG112960.2241181794842187No Hit
AACTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTTG105300.208920363842849No Hit
AGGCCGAGAGCTGATGACGAGTTACCTTGGAATTCTCGGGTGCCAAGGA102810.20398008173488422RNA PCR Primer, Index 1 (100% over 22bp)
AAGTTAGCGGATGACCTGTGGATCGGTGGAATTCTCGGGTGCCAAGGAAC101970.20231348054183584RNA PCR Primer, Index 1 (100% over 24bp)
AAAAGTTAGCGGATGACCTGTGGATCGGTGGAATTCTCGGGTGCCAAGG100800.19999214316580421Illumina Small RNA Adapter 2 (100% over 21bp)
TGAGATTTGAGAGGGGCTGCTCCTTGGAATTCTCGGGTGCCAAGGAACTC95350.18917907590138325RNA PCR Primer, Index 1 (100% over 26bp)
CAGATAACCAGGAACCGTACCCCAAACCGATGGAATTCTCGGGTGCCA94120.18673869558299097No Hit
TCCCCATGTGAGAGTAGGTCATCGTCAAGATTGGAATTCTCGGGTGCCA92270.1830682048602059No Hit
AAAGTTAGCGGATGACCTGTGGATCGGTGGAATTCTCGGGTGCCAAGGA89200.17697717430942198RNA PCR Primer, Index 1 (100% over 22bp)
CCCCATGTGAGAGTAGGTCATCGTCAAGTGGAATTCTCGGGTGCCAAGG88450.1754891375299145Illumina Small RNA Adapter 2 (100% over 21bp)
GAACTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTG88430.17544945654912764No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTGGAATTCTCGGG87060.17273130936522732No Hit
TCCCCATGTGAGAGTAGGTCATCGTCAAGTGGAATTCTCGGGTGCCAAGG85720.1700726836525073Illumina Small RNA Adapter 2 (100% over 21bp)
CAGATAACCAGGAACCGTACCCCAAACCGACACTGGAATTCTCGGGTGCC83380.165430008900444No Hit
ACTCTGCAATTTTGACGTTACCGACAGAATAAGCACCGGCTAACTCTGTT81400.16150159180254425No Hit
TGTTCTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTGGAATTCT81120.16094605807152815No Hit
ACAGGTGGTTGGGTAGAGAATACCAAGGCGCTGGAATTCTCGGGTGCCA80280.15927945687847977No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCGCTCTGGAATTCTCGGGTGCC79380.1574938127430708No Hit
AGGTGGTTGGGTAGAGAATACCAAGGCGCTGGAATTCTCGGGTGCCAAGG77160.1530892238757287Illumina Small RNA Adapter 2 (100% over 21bp)
AGTTAGCGGATGACCTGTGGATCGGTGGAATTCTCGGGTGCCAAGGAACT76780.15233528524077825RNA PCR Primer, Index 1 (100% over 25bp)
ATCTGCCGTGGACGTTTGAGATTTGAGAGGGGTGGAATTCTCGGGTGCC76250.15128373924992627No Hit
AAGGCCGAGAGCTGATGACGAGTTACCTTGGAATTCTCGGGTGCCAAGG76030.15084724846127076Illumina Small RNA Adapter 2 (100% over 21bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGGAATTCTCG75690.15017267178789404No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGTGGAATTCTCGGGTGCCAAGG74600.14801005833500985Illumina Small RNA Adapter 2 (100% over 21bp)
TTGAGAGAACTCGGGTGAAGGAACTAGGCAAAATGGCACCGTGGAATTCT74490.14779181294068208No Hit
GTTCTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTGGAATTCTC74290.14739500313281342No Hit
TAACACATGCAAGTCGAGCGGATGAGAGGAGCTTGGAATTCTCGGGTGCC73010.144855420362454No Hit
ACTACTGACTGACCGATAGTGAACTTGGAATTCTCGGGTGCCAAGGAACT71020.14090716277416085RNA PCR Primer, Index 1 (100% over 25bp)
TGGCTCAGATTGAACGCTGGCGGCAGGCCTGGAATTCTCGGGTGCCAAGG69880.1386453468693095Illumina Small RNA Adapter 2 (100% over 21bp)
TCTAAGCGGGAAACTTGCCTCAAGATGAGATCTCACTTGGAATTCTCGGG68820.1365422548876056No Hit
TCATGGCTCAGATTGAACGCTGGCGGCAGGCCTGGAATTCTCGGGTGCC66110.13116548199098527No Hit
AGATCCTGAAACCGTATGCGTACAAGTGGAATTCTCGGGTGCCAAGGAAC64300.1275743532297739RNA PCR Primer, Index 1 (100% over 24bp)
CAGGTGGTTGGGTAGAGAATACCAAGGCGCTGGAATTCTCGGGTGCCA64050.1270783409699381No Hit
TGTTCTGTGACTGCGTACCTTTTGTATATGGAATTCTCGGGTGCCAAGG63180.12535221830570942Illumina Small RNA Adapter 2 (100% over 21bp)
CCAAGTTTAAGGTGGTAGGCTGAGATCTTAGGTTGGAATTCTCGGGTGCC62930.12485620604587361No Hit
TAAATCAGTTATGGTTCCTTTGGTCGCTCGCTCTGGAATTCTCGGGTGCC62250.12350705269912016No Hit
AATACTACTGACTGACCGATAGTGAACTTGGAATTCTCGGGTGCCAAGG62000.12301104043928435Illumina Small RNA Adapter 2 (100% over 21bp)
TTCTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTGGAATTCTCG61000.12102699139994104No Hit
GAACTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTT60100.11924134726453207No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC58630.11632479517669743No Hit
TGCCTCAAGATGAGATCTCACTGGAACCTGGAATTCTCGGGTGCCAAGG57940.11495580133955056Illumina Small RNA Adapter 2 (100% over 21bp)
TAAGTGGCTTTGAGATTAGCGGAACGCTCTGGAAAGTGCGGCCATTGGA57220.11352728603122336No Hit
TGGCTTTGAGATTAGCGGAACGCTCTGGAAAGTGCGGCCATAGTTGGAAT56820.11273366641548604No Hit
ACTTGCCTCAAGATGAGATCTCACTTGGAATTCTCGGGTGCCAAGGAACT56190.11148371552069979RNA PCR Primer, Index 1 (100% over 25bp)
AACACATGCAAGTCGAGCGGATGAGAGGAGCTTGGAATTCTCGGGTGCC55850.11080913884732306No Hit
TACCAAGGCGCTTGAGAGAACTCGGGTGAAGGAACTTGGAATTCTCGGGT55650.1104123290394544No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAT55440.1099956787411923No Hit
GAAAAGTTAGCGGATGACCTGTGGATCGGTGGAATTCTCGGGTGCCAAGG54960.10904333520230752Illumina Small RNA Adapter 2 (100% over 21bp)
TCTTGACGACCATAGAGCGTTGGAACCACCTTGGAATTCTCGGGTGCCA54070.107277531557292No Hit
AAATCCGGGATCTTAAGGCCGAGAGCTGATTGGAATTCTCGGGTGCCA53450.10604742115289914No Hit
TAAGTGGCTTTGAGATTAGCGGAACGCTCTGGAAAGTGCGGCCATAGTTG53290.10572997330660422No Hit
TCTTGACGACCATAGAGCGTTGGAACCACCTGATGGAATTCTCGGGTGCC53280.10571013281621079No Hit
TCTGCCGTGGACGTTTGAGATTTGAGAGGGGTGGAATTCTCGGGTGCCA52850.10485699172929318No Hit
TCTTGACGACCATAGAGCGTTGGAACCACCTGATTGGAATTCTCGGGTGC51780.10273405925719586No Hit
TTCTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTGGAATTCTC51780.10273405925719586No Hit
CGGGAAACTTGCCTCAAGATGAGATCTCACTTGGAATTCTCGGGTGCCA51000.10118650100650807No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)