FastQCFastQC Report
Fri 29 Sept 2023
SRR6053477_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6053477_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5675996
Total Bases281 Mbp
Sequences flagged as poor quality0
Sequence length38-50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAACACATGCAAGTCGAGCGGTGGAATTCTCGGGTGCCAAGGAACTCC453610.7991725152730904RNA PCR Primer, Index 1 (100% over 27bp)
AGCGGGAAACTTGCCTCAAGATGAGATCTCACTTGGAATTCTCGGGTGCC414350.730004038057814No Hit
GTCAACTAGCCGTTGGGAGCCTGGAATTCTCGGGTGCCAAGGAACTCC296580.5225162244652745RNA PCR Primer, Index 1 (100% over 27bp)
CCCCATGTGAGAGTAGGTCATCGTCAAGATTGGAATTCTCGGGTGCCA288270.5078756221815519No Hit
TTCTGTGACTGCGTACCTTTTGTATATGGAATTCTCGGGTGCCAAGGAAC225990.39815038629343646RNA PCR Primer, Index 1 (100% over 24bp)
TGAGATTTGAGAGGGGCTGCTCCTTGGAATTCTCGGGTGCCAAGGAACTC219860.38735051962686373RNA PCR Primer, Index 1 (100% over 26bp)
ATACTACTGACTGACCGATAGTGAACTTGGAATTCTCGGGTGCCAAGGA200450.35315387819159844RNA PCR Primer, Index 1 (100% over 22bp)
TTGAGAGAACTCGGGTGAAGGAACTTGGAATTCTCGGGTGCCAAGGAACT199540.35155063534223774RNA PCR Primer, Index 1 (100% over 25bp)
CTCAGATTGAACGCTGGCGGCAGGCCTGGAATTCTCGGGTGCCAAGGAAC168380.2966527812916006RNA PCR Primer, Index 1 (100% over 24bp)
TGCCTCAAGATGAGATCTCACTGGAACCTGGAATTCTCGGGTGCCAAGG160580.28291069972565164Illumina Small RNA Adapter 2 (100% over 21bp)
CAGATAACCAGGAACCGTACCCCAAACCGACTGGAATTCTCGGGTGCCA146920.25884443893195136No Hit
TCTAAGCGGGAAACTTGCCTCAAGATGAGATCTCACTTGGAATTCTCGGG140970.24836169722459284No Hit
AGGCCGAGAGCTGATGACGAGTTACCTTGGAATTCTCGGGTGCCAAGGA139240.2453137740054785RNA PCR Primer, Index 1 (100% over 22bp)
AGCGGGAAACTTGCCTCAAGATGAGATCTCACTGGAACCTGGAATTCTCG137590.2424067952126816No Hit
CGGGGCTCAAACCATACACCGAAGCTACGGGTGGAATTCTCGGGTGCCA136970.24131447590872157No Hit
AAATCCGGGATCTTAAGGCCGAGAGCTGATGACGAGTTACCTTGGAATTC135610.23891842066132535No Hit
CCCCATGTGAGAGTAGGTCATCGTCAAGTGGAATTCTCGGGTGCCAAGG134390.23676901815998463Illumina Small RNA Adapter 2 (100% over 21bp)
GGTGGTTGGGTAGAGAATACCAAGGCGCTGGAATTCTCGGGTGCCAAGG130470.2298627412704308Illumina Small RNA Adapter 2 (100% over 21bp)
AAGGCGCTTGAGAGAACTCGGGTGAAGGAACTTGGAATTCTCGGGTGCC129480.22811855399475267No Hit
CAGATAACCAGGAACCGTACCCCAAACCGACATGGAATTCTCGGGTGCC129210.22764286655593136No Hit
AATACCAAGGCGCTTGAGAGAACTCGGGTGAAGGAACTTGGAATTCTCGG128850.22700861663750294No Hit
AAGGCCGAGAGCTGATGACGAGTTACCTTGGAATTCTCGGGTGCCAAGG126920.22360833235259503Illumina Small RNA Adapter 2 (100% over 21bp)
TGTCAACTAGCCGTTGGGAGCCTGGAATTCTCGGGTGCCAAGGAACTCC120220.21180423664851067RNA PCR Primer, Index 1 (100% over 27bp)
CTATGTTCGGGAAAGATAACCGCTTGGAATTCTCGGGTGCCAAGGAACTC118760.20923200086821764RNA PCR Primer, Index 1 (100% over 26bp)
TCCCCATGTGAGAGTAGGTCATCGTCAAGATTGGAATTCTCGGGTGCCA114820.20229048787208445No Hit
TACTACTGACTGACCGATAGTGAACTTGGAATTCTCGGGTGCCAAGGAAC114060.20095151582206894RNA PCR Primer, Index 1 (100% over 24bp)
ACTACTGACTGACCGATAGTGAACTTGGAATTCTCGGGTGCCAAGGAACT110760.19513755823647513RNA PCR Primer, Index 1 (100% over 25bp)
AACACATGCAAGTCGAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT109820.1934814612272454RNA PCR Primer, Index 1 (100% over 30bp)
TCCCCATGTGAGAGTAGGTCATCGTCAAGTGGAATTCTCGGGTGCCAAGG108800.19168441979169823Illumina Small RNA Adapter 2 (100% over 21bp)
CTTGCCTCAAGATGAGATCTCACTTGGAATTCTCGGGTGCCAAGGAACTC106770.18810795497389357RNA PCR Primer, Index 1 (100% over 26bp)
AGCGGAACGCTCTGGAAAGTGCGGCCTGGAATTCTCGGGTGCCAAGGAAC106130.18698039956335416RNA PCR Primer, Index 1 (100% over 24bp)
CTCAGATTGAACGCTGGCGGCAGGCCTTGGAATTCTCGGGTGCCAAGGA105350.18560619140675927RNA PCR Primer, Index 1 (100% over 22bp)
TCATGGCTCAGATTGAACGCTGGCGGCAGGCCTGGAATTCTCGGGTGCC104840.18470767068898566No Hit
TGCCTCAAGATGAGATCTCACTTGGAATTCTCGGGTGCCAAGGAACTCC104650.1843729276764818RNA PCR Primer, Index 1 (100% over 27bp)
ATGTTCGGGAAAGATAACCGCTTGGAATTCTCGGGTGCCAAGGAACTCC102030.17975699771458614RNA PCR Primer, Index 1 (100% over 27bp)
AGCGGATGACCTGTGGATCGGTGGAATTCTCGGGTGCCAAGGAACTCC101950.17961605328826868RNA PCR Primer, Index 1 (100% over 27bp)
ACCAAGGCGCTTGAGAGAACTCGGGTGAAGGAACTTGGAATTCTCGGGTG97680.17209314453357613No Hit
AAAAGTTAGCGGATGACCTGTGGATCGGTGGAATTCTCGGGTGCCAAGG96140.1693799643269657Illumina Small RNA Adapter 2 (100% over 21bp)
ACTTGCCTCAAGATGAGATCTCACTTGGAATTCTCGGGTGCCAAGGAACT93470.16467594409862163RNA PCR Primer, Index 1 (100% over 25bp)
ATCTGCCGTGGACGTTTGAGATTTGAGAGGGGTGGAATTCTCGGGTGCC89400.1575053964097226No Hit
AGCTATGTTCGGGAAAGATAACCGCTTGGAATTCTCGGGTGCCAAGGAAC88980.15676543817155614RNA PCR Primer, Index 1 (100% over 24bp)
AGGCGCTTGAGAGAACTCGGGTGAAGGAACTTGGAATTCTCGGGTGCCA83380.1468993283293364No Hit
CTTGCCTCAAGATGAGATCTCACTGGAACCTGGAATTCTCGGGTGCCA82700.14570130070563828No Hit
AGATCCTGAAACCGTATGCGTACAAGTGGAATTCTCGGGTGCCAAGGAAC81940.14436232865562273RNA PCR Primer, Index 1 (100% over 24bp)
TACCAAGGCGCTTGAGAGAACTCGGGTGAAGGAACTTGGAATTCTCGGGT80990.1426886135931033No Hit
CGGGAAACTTGCCTCAAGATGAGATCTCACTTGGAATTCTCGGGTGCCA80950.1426181413799446No Hit
GATGTCAACTAGCCGTTGGGAGCCTGGAATTCTCGGGTGCCAAGGAACTC80040.14101489853058388RNA PCR Primer, Index 1 (100% over 26bp)
AAGTTAGCGGATGACCTGTGGATCGGTGGAATTCTCGGGTGCCAAGGAAC79230.13958783621411996RNA PCR Primer, Index 1 (100% over 24bp)
ACAGGTGGTTGGGTAGAGAATACCAAGGCGCTGGAATTCTCGGGTGCCA78590.13846028080358055No Hit
AAAGTTAGCGGATGACCTGTGGATCGGTGGAATTCTCGGGTGCCAAGGA77700.13689227406079918RNA PCR Primer, Index 1 (100% over 22bp)
TGTTCTGTGACTGCGTACCTTTTGTATATGGAATTCTCGGGTGCCAAGG76950.13557092006407334Illumina Small RNA Adapter 2 (100% over 21bp)
TAACACATGCAAGTCGAGCGGATGAGAGGAGCTTGGAATTCTCGGGTGCC76080.13403814942787134No Hit
ACGATGTCAACTAGCCGTTGGGAGCCTGGAATTCTCGGGTGCCAAGGAAC75650.13328057313641517RNA PCR Primer, Index 1 (100% over 24bp)
TAGCGGATGACCTGTGGATCGGTGGAATTCTCGGGTGCCAAGGAACTCC75460.1329458301239113RNA PCR Primer, Index 1 (100% over 27bp)
TTGAGATTTGAGAGGGGCTGCTCCTTGGAATTCTCGGGTGCCAAGGAACT74640.13150114975415766RNA PCR Primer, Index 1 (100% over 25bp)
AATACTACTGACTGACCGATAGTGAACTTGGAATTCTCGGGTGCCAAGG72000.12684998368568265Illumina Small RNA Adapter 2 (100% over 21bp)
TTCTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTGGAATTCTCG70700.12455963675802449No Hit
GCCGAGAGCTGATGACGAGTTACCTTGGAATTCTCGGGTGCCAAGGAACT70520.1242425117988103RNA PCR Primer, Index 1 (100% over 25bp)
TCTGTGACTGCGTACCTTTTGTATATGGAATTCTCGGGTGCCAAGGAACT68230.120207977595474RNA PCR Primer, Index 1 (100% over 25bp)
AGAGAACTCGGGTGAAGGAACTTGGAATTCTCGGGTGCCAAGGAACTCC67960.11973229015665267RNA PCR Primer, Index 1 (100% over 27bp)
AGATCCTGAAACCGTATGCGTACTGGAATTCTCGGGTGCCAAGGAACTCC65950.11619106144542739RNA PCR Primer, Index 1 (100% over 27bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTTGGAATTCTCGG65700.11575061011318542No Hit
TGGCTCAGATTGAACGCTGGCGGCAGGCCTGGAATTCTCGGGTGCCAAGG65410.11523968656778476Illumina Small RNA Adapter 2 (100% over 21bp)
TTCTGTGACTGCGTACCTTTTGTATGGAATTCTCGGGTGCCAAGGAACTC63830.11245603414801561RNA PCR Primer, Index 1 (100% over 26bp)
TCTGCCGTGGACGTTTGAGATTTGAGAGGGGTGGAATTCTCGGGTGCCA63510.1118922564427459No Hit
AAACTTTGTCTGAATATGGGGGGACCTGGAATTCTCGGGTGCCAAGGAAC62640.11035948580654391RNA PCR Primer, Index 1 (100% over 24bp)
AACACATGCAAGTCGAGCGGATGAGAGGAGCTTGGAATTCTCGGGTGCC61890.10903813180981804No Hit
AGAGCGTTGGAACCACCTGATCCCATCCCGAACTCAGATGGAATTCTCGG61670.10865053463744512No Hit
ATACCAAGGCGCTTGAGAGAACTCGGGTGAAGGAACTTGGAATTCTCGGG60960.10739965285387798No Hit
CAGATAACCAGGAACCGTACCCCAAACCGATGGAATTCTCGGGTGCCA60960.10739965285387798No Hit
CCAAGTTTAAGGTGGTAGGCTGAGATCTTAGGTTGGAATTCTCGGGTGCC60550.10667731266900118No Hit
GCGCTTGAGAGAACTCGGGTGAAGGAACTTGGAATTCTCGGGTGCCAAGG59680.10514454203279919Illumina Small RNA Adapter 2 (100% over 21bp)
AGGTGGTTGGGTAGAGAATACCAAGGCGCTGGAATTCTCGGGTGCCAAGG59120.10415793104857721Illumina Small RNA Adapter 2 (100% over 21bp)
TATCTGCCGTGGACGTTTGAGATTTGAGAGGGGTGGAATTCTCGGGTGCC58540.10313608395777588No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC58360.10281895899856167No Hit
GGCAAACAGACTTTGATCCGGAGATGTCTTGGAATTCTCGGGTGCCAAGG58340.1027837228919823Illumina Small RNA Adapter 2 (100% over 21bp)
AAATCCGGGATCTTAAGGCCGAGAGCTGATTGGAATTCTCGGGTGCCA58310.10273086873211328No Hit
TCATGGCTCAGATTGAACGCTGGCGGCAGGCCTTGGAATTCTCGGGTGCC57330.10100429950972481No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)