FastQCFastQC Report
Thu 28 Sept 2023
SRR6053478_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6053478_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6618749
Total Bases328 Mbp
Sequences flagged as poor quality0
Sequence length37-50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGGGAAACTTGCCTCAAGATGAGATCTCACTTGGAATTCTCGGGTGCC249040.37626445722598034No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGA229370.34654585028077056No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTTGGAATTCTCGG213580.32268937831000993No Hit
TGAGATTTGAGAGGGGCTGCTCCTTGGAATTCTCGGGTGCCAAGGAACTC187960.28398115716429195RNA PCR Primer, Index 1 (100% over 26bp)
GTCAACTAGCCGTTGGGAGCCTGGAATTCTCGGGTGCCAAGGAACTCC170640.2578130701134006RNA PCR Primer, Index 1 (100% over 27bp)
TTGAGAGAACTCGGGTGAAGGAACTTGGAATTCTCGGGTGCCAAGGAACT150730.22773185688111153RNA PCR Primer, Index 1 (100% over 25bp)
CTCAGATTGAACGCTGGCGGCAGGCCTGGAATTCTCGGGTGCCAAGGAAC144970.21902930599120768RNA PCR Primer, Index 1 (100% over 24bp)
TCTGAATGGGGGAACCCAGCCATCACAAGATGGTTATCTTGTACTTGGA143350.21658171355342226No Hit
AAATCCGGGATCTTAAGGCCGAGAGCTGATGACGAGTTACCTTGGAATTC135030.20401136226800565No Hit
CAGATAACCAGGAACCGTACCCCAAACCGACTGGAATTCTCGGGTGCCA128520.1941756667309789No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTGGAATTCTCGGG126480.19109351329080465No Hit
CAGATAACCAGGAACCGTACCCCAAACCGACATGGAATTCTCGGGTGCC124990.18884233259185385No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCGCTCTGGAATTCTCGGGTGCC119730.18089521146669862No Hit
AGCAGGGTCGGGCCTGGTTAGTACTTGGATGGAATTCTCGGGTGCCAAGG119270.18020021608312992Illumina Small RNA Adapter 2 (100% over 21bp)
GGCGGCGCTGTCTCCACCCGAGACTCAGTGGAATTCTCGGGTGCCAAGG119100.17994336996311538Illumina Small RNA Adapter 2 (100% over 21bp)
AGATCCTGAAACCGTATGCGTACAAGTGGAATTCTCGGGTGCCAAGGAAC118020.1783116416712584RNA PCR Primer, Index 1 (100% over 24bp)
TCCTACTTGGATAACTGTGGTAATTCTAGTGGAATTCTCGGGTGCCAAGG117330.17726914859590537Illumina Small RNA Adapter 2 (100% over 21bp)
GGTGGTTGGGTAGAGAATACCAAGGCGCTGGAATTCTCGGGTGCCAAGG112380.16979039392489426Illumina Small RNA Adapter 2 (100% over 21bp)
ATACTACTGACTGACCGATAGTGAACTTGGAATTCTCGGGTGCCAAGGA112060.16930691887545515RNA PCR Primer, Index 1 (100% over 22bp)
CTATGTTCGGGAAAGATAACCGCTTGGAATTCTCGGGTGCCAAGGAACTC110750.16732769289181385RNA PCR Primer, Index 1 (100% over 26bp)
TGGCGGCGCTGTCTCCACCCGAGACTCAGTGGAATTCTCGGGTGCCAAGG102030.15415299779459835Illumina Small RNA Adapter 2 (100% over 21bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC101630.1535486539827995No Hit
TACTACTGACTGACCGATAGTGAACTTGGAATTCTCGGGTGCCAAGGAAC100630.1520377944533023RNA PCR Primer, Index 1 (100% over 24bp)
TAGCGGATGACCTGTGGATCGGTGGAATTCTCGGGTGCCAAGGAACTCC100330.15158453659445312RNA PCR Primer, Index 1 (100% over 27bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCC99290.15001324268377603No Hit
AGCGGATGACCTGTGGATCGGTGGAATTCTCGGGTGCCAAGGAACTCC97780.1477318447942353RNA PCR Primer, Index 1 (100% over 27bp)
CCCCATGTGAGAGTAGGTCATCGTCAAGATTGGAATTCTCGGGTGCCA96870.14635696262239284No Hit
AATACCAAGGCGCTTGAGAGAACTCGGGTGAAGGAACTTGGAATTCTCGG96580.14591881335883866No Hit
CGGGGCTCAAACCATACACCGAAGCTACGGGTGGAATTCTCGGGTGCCA95490.1442719764716867No Hit
CTTGCCTCAAGATGAGATCTCACTTGGAATTCTCGGGTGCCAAGGAACTC94860.14332013496810347RNA PCR Primer, Index 1 (100% over 26bp)
AGCAGGGTCGGGCCTGGTTAGTACTTGGTGGAATTCTCGGGTGCCAAGG91810.13871201340313705Illumina Small RNA Adapter 2 (100% over 21bp)
TCTTGACGACCATAGAGCGTTGGAACCACCTTGGAATTCTCGGGTGCCA91030.13753354297012924No Hit
TCCGGAGATGTCTGAATGGGGGAACCCAGCCATCATGGAATTCTCGGGTG90670.13698963353951024No Hit
AGTTAGCGGATGACCTGTGGATCGGTGGAATTCTCGGGTGCCAAGGAACT82390.12447971663527352RNA PCR Primer, Index 1 (100% over 25bp)
TACCAAGGCGCTTGAGAGAACTCGGGTGAAGGAACTTGGAATTCTCGGGT82240.12425308770584893No Hit
CAGATAACCAGGAACCGTACCCCAAACCGATGGAATTCTCGGGTGCCA79600.12026441854797636No Hit
GGCGGCGCTGTCTCCACCCGAGACTCAGTTGGAATTCTCGGGTGCCAAGG78560.11869312463729928Illumina Small RNA Adapter 2 (100% over 21bp)
TGGCTCAGATTGAACGCTGGCGGCAGGCCTGGAATTCTCGGGTGCCAAGG77760.11748443701370154Illumina Small RNA Adapter 2 (100% over 21bp)
AAGTTAGCGGATGACCTGTGGATCGGTGGAATTCTCGGGTGCCAAGGAAC77450.11701607055955741RNA PCR Primer, Index 1 (100% over 24bp)
AAAACACAGCACTCTGCAAACACGAAAGTGGACGTGGAATTCTCGGGTGC77300.11678944163013283No Hit
AGCGGAACGCTCTGGAAAGTGCGGCCTGGAATTCTCGGGTGCCAAGGAAC76890.11616998922303898RNA PCR Primer, Index 1 (100% over 24bp)
TGGCGGCGCTGTCTCCACCCGAGACTCAGTTGGAATTCTCGGGTGCCA76740.1159433602936144No Hit
TGTCAACTAGCCGTTGGGAGCCTGGAATTCTCGGGTGCCAAGGAACTCC76490.1155656454112401RNA PCR Primer, Index 1 (100% over 27bp)
AGCTATGTTCGGGAAAGATAACCGCTTGGAATTCTCGGGTGCCAAGGAAC76340.11533901648181552RNA PCR Primer, Index 1 (100% over 24bp)
TAAATCAGTTATGGTTCCTTTGGTCGCTCGCTCTGGAATTCTCGGGTGCC76340.11533901648181552No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGC75930.11471956407472167No Hit
AAAAGTTAGCGGATGACCTGTGGATCGGTGGAATTCTCGGGTGCCAAGG75550.11414543745351274Illumina Small RNA Adapter 2 (100% over 21bp)
ACTACTGACTGACCGATAGTGAACTTGGAATTCTCGGGTGCCAAGGAACT75040.11337489909346916RNA PCR Primer, Index 1 (100% over 25bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTTGGAATTCTCGGGTG74690.11284609825814515No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC74240.11216621146987141No Hit
ACTTGCCTCAAGATGAGATCTCACTTGGAATTCTCGGGTGCCAAGGAACT71500.10802645635904912RNA PCR Primer, Index 1 (100% over 25bp)
ATACCAAGGCGCTTGAGAGAACTCGGGTGAAGGAACTTGGAATTCTCGGG70510.1065307054248469No Hit
CAGATAACCAGGAACCGTACCCCAAACCGACACATGGAATTCTCGGGTGC70130.10595657880363796No Hit
ACGGCCCTGGCGGAGCGCTGAGAAGACGGTCGAACTTGACTATCTTGGA70110.10592636161304801No Hit
ACCAAGGCGCTTGAGAGAACTCGGGTGAAGGAACTTGGAATTCTCGGGTG69260.10464213101297543No Hit
TGGCTTTGAGATTAGCGGAACGCTCTGGAAAGTGCGGCCATTGGAATTCT69200.10455147944120559No Hit
AAAGTTAGCGGATGACCTGTGGATCGGTGGAATTCTCGGGTGCCAAGGA66860.10101606814218216RNA PCR Primer, Index 1 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)