FastQCFastQC Report
Thu 28 Sept 2023
SRR6053479_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6053479_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3899688
Total Bases193.5 Mbp
Sequences flagged as poor quality0
Sequence length40-50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATCCGGGATCTTAAGGCCGAGAGCTGATGACGAGTTACCTTGGAATTC496381.2728710604540672No Hit
CCCCATGTGAGAGTAGGTCATCGTCAAGATTGGAATTCTCGGGTGCCA293440.7524704540465802No Hit
AGCGGGAAACTTGCCTCAAGATGAGATCTCACTTGGAATTCTCGGGTGCC218110.5593011543487582No Hit
GGTGGTTGGGTAGAGAATACCAAGGCGCTGGAATTCTCGGGTGCCAAGG182170.4671399352973879Illumina Small RNA Adapter 2 (100% over 21bp)
AACTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTGG181540.46552442144089473No Hit
TTCTGTGACTGCGTACCTTTTGTATATGGAATTCTCGGGTGCCAAGGAAC165460.424290353484689RNA PCR Primer, Index 1 (100% over 24bp)
CAGATAACCAGGAACCGTACCCCAAACCGACATGGAATTCTCGGGTGCC133300.34182221757227754No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTTGGAATTCTCGG132830.3406169929491795No Hit
CAGATAACCAGGAACCGTACCCCAAACCGACTGGAATTCTCGGGTGCCA132690.34025798986995887No Hit
CCCCATGTGAGAGTAGGTCATCGTCAAGTGGAATTCTCGGGTGCCAAGG126510.3244105682300738Illumina Small RNA Adapter 2 (100% over 21bp)
TCTAAGCGGGAAACTTGCCTCAAGATGAGATCTCACTTGGAATTCTCGGG110110.2823559218070779No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGA109120.2798172571754458No Hit
CAGATAACCAGGAACCGTACCCCAAACCGACACATGGAATTCTCGGGTGC105990.2717909740471545No Hit
AGGCCGAGAGCTGATGACGAGTTACCTTGGAATTCTCGGGTGCCAAGGA104040.266790574015152RNA PCR Primer, Index 1 (100% over 22bp)
CGGGGCTCAAACCATACACCGAAGCTACGGGTGGAATTCTCGGGTGCCA101600.26053366320587695No Hit
GAACTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTG98220.25186630315040587No Hit
TTGAGAGAACTCGGGTGAAGGAACTTGGAATTCTCGGGTGCCAAGGAACT97600.25027643237099995RNA PCR Primer, Index 1 (100% over 25bp)
CTCAGATTGAACGCTGGCGGCAGGCCTGGAATTCTCGGGTGCCAAGGAAC96310.2469684754267521RNA PCR Primer, Index 1 (100% over 24bp)
AAGGCCGAGAGCTGATGACGAGTTACCTTGGAATTCTCGGGTGCCAAGG95360.24453238310346878Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCCATGTGAGAGTAGGTCATCGTCAAGATTGGAATTCTCGGGTGCCA94350.24194243231766233No Hit
AAAAGTTAGCGGATGACCTGTGGATCGGTGGAATTCTCGGGTGCCAAGG93300.23924990922350708Illumina Small RNA Adapter 2 (100% over 21bp)
TCCTCCAAGGCTAAATACTACTGACTGACCGATAGTGAACTTGGAATTCT91100.2336084322643247No Hit
TCCCCATGTGAGAGTAGGTCATCGTCAAGTGGAATTCTCGGGTGCCAAGG90690.23255706610374985Illumina Small RNA Adapter 2 (100% over 21bp)
ACAGGTGGTTGGGTAGAGAATACCAAGGCGCTGGAATTCTCGGGTGCCA90270.23148005686608775No Hit
ATACTACTGACTGACCGATAGTGAACTTGGAATTCTCGGGTGCCAAGGA89270.2289157491573685RNA PCR Primer, Index 1 (100% over 22bp)
AAGTTAGCGGATGACCTGTGGATCGGTGGAATTCTCGGGTGCCAAGGAAC84170.21583777984290026RNA PCR Primer, Index 1 (100% over 24bp)
CCAAGTTTAAGGTGGTAGGCTGAGATCTTAGGTTGGAATTCTCGGGTGCC82940.21268368136117557No Hit
AAAGTTAGCGGATGACCTGTGGATCGGTGGAATTCTCGGGTGCCAAGGA80560.2065806290144237RNA PCR Primer, Index 1 (100% over 22bp)
TGAGATTTGAGAGGGGCTGCTCCTTGGAATTCTCGGGTGCCAAGGAACTC78970.20250337975756008RNA PCR Primer, Index 1 (100% over 26bp)
AGCGGGAAACTTGCCTCAAGATGAGATCTCACTGGAACCTGGAATTCTCG78170.20045193359058466No Hit
TGGCGGCGCTGTCTCCACCCGAGACTCAGTGAAATTGAAATCGCTGTGA75990.1948617427855767No Hit
TAAGTGGCTTTGAGATTAGCGGAACGCTCTGGAAAGTGCGGCCATAGTTG74110.19004084429318446No Hit
CAGATAACCAGGAACCGTACCCCAAACCGATGGAATTCTCGGGTGCCA74050.18988698583066133No Hit
TGTTCTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTGGAATTCT73920.1895536258285278No Hit
AGGTGGTTGGGTAGAGAATACCAAGGCGCTGGAATTCTCGGGTGCCAAGG72800.18668160119476224Illumina Small RNA Adapter 2 (100% over 21bp)
ATCTGCCGTGGACGTTTGAGATTTGAGAGGGGTGGAATTCTCGGGTGCC71510.1833736442505144No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGGAATTCTCG70330.18034776115422566No Hit
TTCTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTGGAATTCTCG70070.17968104114995867No Hit
TAACACATGCAAGTCGAGCGGATGAGAGGAGCTTGGAATTCTCGGGTGCC69620.178527102681035No Hit
TTCGGTCCCTATCTGCCGTGGACGTTTGAGATTTGAGAGGGGTGGAATTC68830.17650129959114677No Hit
TGTTCTGTGACTGCGTACCTTTTGTATATGGAATTCTCGGGTGCCAAGG67200.17232147802593437Illumina Small RNA Adapter 2 (100% over 21bp)
TCTTGACGACCATAGAGCGTTGGAACCACCTGATCCCATCCCGAACTC66420.17032131801313335No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTGGAATTCTCGGG65120.16698771799179832No Hit
AAACTTTGTCTGAATATGGGGGGACCTGGAATTCTCGGGTGCCAAGGAAC62570.1604487333345642RNA PCR Primer, Index 1 (100% over 24bp)
GTTCTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTGGAATTCTC60940.15626891176935181No Hit
CAGGTGGTTGGGTAGAGAATACCAAGGCGCTGGAATTCTCGGGTGCCA59140.15165315789365713No Hit
CGACCACCTGGACTGATACTGACACTGAGGTGCGTGGAATTCTCGGGTGC58040.14883241941406597No Hit
CAGATAACCAGGAACCGTACCCCAAACCGACACTGGAATTCTCGGGTGCC57070.14634504093660824No Hit
AAATCCGGGATCTTAAGGCCGAGAGCTGATTGGAATTCTCGGGTGCCA56530.14496031477389987No Hit
TACTACTGACTGACCGATAGTGAACTTGGAATTCTCGGGTGCCAAGGAAC56280.14431923784672004RNA PCR Primer, Index 1 (100% over 24bp)
TCTGTGACTGCGTACCTTTTGTATATGGAATTCTCGGGTGCCAAGGAACT54820.14057534859198992RNA PCR Primer, Index 1 (100% over 25bp)
TCATGGCTCAGATTGAACGCTGGCGGCAGGCCTGGAATTCTCGGGTGCC54480.13970348397102536No Hit
GAAAAGTTAGCGGATGACCTGTGGATCGGTGGAATTCTCGGGTGCCAAGG53870.13813925626870663Illumina Small RNA Adapter 2 (100% over 21bp)
AGTTAGCGGATGACCTGTGGATCGGTGGAATTCTCGGGTGCCAAGGAACT53220.1364724562580391RNA PCR Primer, Index 1 (100% over 25bp)
AATACCAAGGCGCTTGAGAGAACTCGGGTGAAGGAACTTGGAATTCTCGG52120.13365171777844792No Hit
ACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTTGGA51730.13265163777204741No Hit
AGCATCTAAGCGGGAAACTTGCCTCAAGATGAGATCTCACTTGGAATTCT51300.13154898545729812No Hit
AACTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTTG50860.13042069006546164No Hit
AGAGCGTTGGAACCACCTGATCCCATCCCGAACTCAGATGGAATTCTCGG50700.13001040083206655No Hit
CGGGAAACTTGCCTCAAGATGAGATCTCACTTGGAATTCTCGGGTGCCA48660.12477921310627928No Hit
TCGGTCCCTATCTGCCGTGGACGTTTGAGATTTGAGAGGGGTGGAATTCT48570.12454842541249453No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCGCTCTGGAATTCTCGGGTGCC48530.12444585310414577No Hit
AAGTGGCTTTGAGATTAGCGGAACGCTCTGGAAAGTGCGGCCATAGTTGG47490.12177897308707772No Hit
TTCCCCATGTGAGAGTAGGTCATCGTCAAGATTGGAATTCTCGGGTGCC47360.12144561308494423No Hit
TTCTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTGGAATTCTC47150.12090710846611318No Hit
TGCCTCAAGATGAGATCTCACTGGAACCTGGAATTCTCGGGTGCCAAGG46840.12011217307641021Illumina Small RNA Adapter 2 (100% over 21bp)
ACTACTGACTGACCGATAGTGAACTTGGAATTCTCGGGTGCCAAGGAACT46830.12008652999932302RNA PCR Primer, Index 1 (100% over 25bp)
ACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAAT46480.11918902230127128No Hit
TGAAAAGTTAGCGGATGACCTGTGGATCGGTGGAATTCTCGGGTGCCA46290.11870180383661462No Hit
CCCATGTGAGAGTAGGTCATCGTCAAGATTGGAATTCTCGGGTGCCAAGG46080.11816329921778357Illumina Small RNA Adapter 2 (100% over 21bp)
TCTGCCGTGGACGTTTGAGATTTGAGAGGGGTGGAATTCTCGGGTGCCA45740.11729143459681902No Hit
ACTTGCCTCAAGATGAGATCTCACTTGGAATTCTCGGGTGCCAAGGAACT45280.11611185305080818RNA PCR Primer, Index 1 (100% over 25bp)
AAAACCGATCGTAGTCCGGATCGCAGTCTGCAACTCGACTGCGTGAAGTC44880.11508612996732047No Hit
GGTGGTTGGGTAGAGAATACCAAGGCGCTTGAGAGTGGAATTCTCGGGTG44690.1145989115026638No Hit
GGCAAACAGACTTTGATCCGGAGATGTCTGTGGAATTCTCGGGTGCCA44510.11413733611509433No Hit
ACTCTGCAATTTTGACGTTACCGACAGAATAAGCACCGGCTAACTCTGTT43300.11103452378754403No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC43190.1107524499395849No Hit
CTCAGATTGAACGCTGGCGGCAGGCCTTGGAATTCTCGGGTGCCAAGGA43000.11026523147492825RNA PCR Primer, Index 1 (100% over 22bp)
TTGAGAGAACTCGGGTGAAGGAACTAGGCAAAATGGCACCGTGGAATTCT42800.1097523699331844No Hit
TATCTGCCGTGGACGTTTGAGATTTGAGAGGGGTGGAATTCTCGGGTGCC42700.10949593916231247No Hit
AACTTTGTCTGAATATGGGGGGACCTGGAATTCTCGGGTGCCAAGGAACT42340.10857278838717355RNA PCR Primer, Index 1 (100% over 25bp)
TCTTGACGACCATAGAGCGTTGGAACCACCTGATTGGAATTCTCGGGTGC41840.10729063453281391No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAT41690.106905988376506No Hit
TCCGGATCGAGGACAGTGTATGGTGGGTAGTTGGAATTCTCGGGTGCCA41270.10582897913884391No Hit
GCATCTAAGCGGGAAACTTGCCTCAAGATGAGATCTCACTTGGAATTCTC41260.10580333606175674No Hit
TGGCTCAGATTGAACGCTGGCGGCAGGCCTGGAATTCTCGGGTGCCAAGG41210.10567512067632077Illumina Small RNA Adapter 2 (100% over 21bp)
AAATCCGGGATCTTAAGGCCGAGAGCTGATGACGAGTTACCTTTGGAATT40770.10454682528448431No Hit
CTTGCCTCAAGATGAGATCTCACTTGGAATTCTCGGGTGCCAAGGAACTC40340.10344417296973502RNA PCR Primer, Index 1 (100% over 26bp)
AAGCGGGAAACTTGCCTCAAGATGAGATCTCACTTGGAATTCTCGGGTGC40090.10280309604255519No Hit
TTCTGTGACTGCGTACCTTTTGTATGGAATTCTCGGGTGCCAAGGAACTC39460.10118758218606205RNA PCR Primer, Index 1 (100% over 26bp)
AACACATGCAAGTCGAGCGGATGAGAGGAGCTTGGAATTCTCGGGTGCC39390.10100808064645171No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)