FastQCFastQC Report
Thu 28 Sept 2023
SRR6053480_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6053480_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6899202
Total Bases341.5 Mbp
Sequences flagged as poor quality0
Sequence length38-50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGGTTGGGTAGAGAATACCAAGGCGCTGGAATTCTCGGGTGCCAAGG893671.2953237200476229Illumina Small RNA Adapter 2 (100% over 21bp)
CCCCATGTGAGAGTAGGTCATCGTCAAGATTGGAATTCTCGGGTGCCA620250.8990170167506328No Hit
GTCAACTAGCCGTTGGGAGCCTGGAATTCTCGGGTGCCAAGGAACTCC483670.7010520926912998RNA PCR Primer, Index 1 (100% over 27bp)
AGCGGGAAACTTGCCTCAAGATGAGATCTCACTTGGAATTCTCGGGTGCC411240.5960689366683277No Hit
CAGATAACCAGGAACCGTACCCCAAACCGACTGGAATTCTCGGGTGCCA402390.5832413661753925No Hit
ACAGGTGGTTGGGTAGAGAATACCAAGGCGCTGGAATTCTCGGGTGCCA358250.5192629524400069No Hit
TTGAGAGAACTCGGGTGAAGGAACTTGGAATTCTCGGGTGCCAAGGAACT346340.5020000863868024RNA PCR Primer, Index 1 (100% over 25bp)
TAACACATGCAAGTCGAGCGGTGGAATTCTCGGGTGCCAAGGAACTCC325550.4718661665508561RNA PCR Primer, Index 1 (100% over 27bp)
AGGTGGTTGGGTAGAGAATACCAAGGCGCTGGAATTCTCGGGTGCCAAGG319360.4628941144207693Illumina Small RNA Adapter 2 (100% over 21bp)
CAGATAACCAGGAACCGTACCCCAAACCGACATGGAATTCTCGGGTGCC309270.44826923461582946No Hit
TAGCGGATGACCTGTGGATCGGTGGAATTCTCGGGTGCCAAGGAACTCC304190.44090606420858525RNA PCR Primer, Index 1 (100% over 27bp)
AGGCCGAGAGCTGATGACGAGTTACCTTGGAATTCTCGGGTGCCAAGGA300930.43618088004960576RNA PCR Primer, Index 1 (100% over 22bp)
CCAAGTTTAAGGTGGTAGGCTGAGATCTTAGGTTGGAATTCTCGGGTGCC265450.3847546426383805No Hit
AAGGCCGAGAGCTGATGACGAGTTACCTTGGAATTCTCGGGTGCCAAGG259250.37576809607835804Illumina Small RNA Adapter 2 (100% over 21bp)
CAGGTGGTTGGGTAGAGAATACCAAGGCGCTGGAATTCTCGGGTGCCA253870.36797009277304826No Hit
AAACTTTGTCTGAATATGGGGGGACCTGGAATTCTCGGGTGCCAAGGAAC251760.3649117680566535RNA PCR Primer, Index 1 (100% over 24bp)
AAGTTAGCGGATGACCTGTGGATCGGTGGAATTCTCGGGTGCCAAGGAAC239370.34695316936654413RNA PCR Primer, Index 1 (100% over 24bp)
AGTTAGCGGATGACCTGTGGATCGGTGGAATTCTCGGGTGCCAAGGAACT238410.3455617040927342RNA PCR Primer, Index 1 (100% over 25bp)
CTCAGATTGAACGCTGGCGGCAGGCCTGGAATTCTCGGGTGCCAAGGAAC237490.34422821653866637RNA PCR Primer, Index 1 (100% over 24bp)
ATACTACTGACTGACCGATAGTGAACTTGGAATTCTCGGGTGCCAAGGA236670.3430396732839537RNA PCR Primer, Index 1 (100% over 22bp)
AGCGGATGACCTGTGGATCGGTGGAATTCTCGGGTGCCAAGGAACTCC224730.32573332394094273RNA PCR Primer, Index 1 (100% over 27bp)
TCTAAGCGGGAAACTTGCCTCAAGATGAGATCTCACTTGGAATTCTCGGG224370.325211524463264No Hit
CAGATAACCAGGAACCGTACCCCAAACCGACACATGGAATTCTCGGGTGC215930.31297822559768507No Hit
ATCTGCCGTGGACGTTTGAGATTTGAGAGGGGTGGAATTCTCGGGTGCC215340.3121230542314894No Hit
TAACACATGCAAGTCGAGCGGATGAGAGGAGCTTGGAATTCTCGGGTGCC214330.31065911680800185No Hit
AAAAGTTAGCGGATGACCTGTGGATCGGTGGAATTCTCGGGTGCCAAGG211730.30689056502476664Illumina Small RNA Adapter 2 (100% over 21bp)
TTCTGTGACTGCGTACCTTTTGTATATGGAATTCTCGGGTGCCAAGGAAC210560.3051947167223108RNA PCR Primer, Index 1 (100% over 24bp)
AAAGTTAGCGGATGACCTGTGGATCGGTGGAATTCTCGGGTGCCAAGGA209540.30371628486888774RNA PCR Primer, Index 1 (100% over 22bp)
AAATCCGGGATCTTAAGGCCGAGAGCTGATTGGAATTCTCGGGTGCCA199860.2896856766913043No Hit
TGAGATTTGAGAGGGGCTGCTCCTTGGAATTCTCGGGTGCCAAGGAACTC198120.2871636458825238RNA PCR Primer, Index 1 (100% over 26bp)
CAGATAACCAGGAACCGTACCCCAAACCGATGGAATTCTCGGGTGCCA193780.2808730632905081No Hit
ACTTGCCTCAAGATGAGATCTCACTTGGAATTCTCGGGTGCCAAGGAACT185350.26865425885486466RNA PCR Primer, Index 1 (100% over 25bp)
CAGATAACCAGGAACCGTACCCCAAACCGACACTGGAATTCTCGGGTGCC180900.2622042375335582No Hit
CGGGGCTCAAACCATACACCGAAGCTACGGGTGGAATTCTCGGGTGCCA176460.2557687106421873No Hit
TCATGGCTCAGATTGAACGCTGGCGGCAGGCCTGGAATTCTCGGGTGCC167060.24214394650279844No Hit
CCCCATGTGAGAGTAGGTCATCGTCAAGTGGAATTCTCGGGTGCCAAGG166300.24104236982769892Illumina Small RNA Adapter 2 (100% over 21bp)
TCCTACTTGGATAACTGTGGTAATTCTAGTGGAATTCTCGGGTGCCAAGG159330.23093975216264143Illumina Small RNA Adapter 2 (100% over 21bp)
TCTGCCGTGGACGTTTGAGATTTGAGAGGGGTGGAATTCTCGGGTGCCA153870.22302579341784745No Hit
AACTTTGTCTGAATATGGGGGGACCTGGAATTCTCGGGTGCCAAGGAACT142380.20637169342193487RNA PCR Primer, Index 1 (100% over 25bp)
AAATCAGTTATGGTTCCTTTGGTCGCTCGCTCTGGAATTCTCGGGTGCC141580.2052121390270933No Hit
TACTACTGACTGACCGATAGTGAACTTGGAATTCTCGGGTGCCAAGGAAC136330.19760256331094522RNA PCR Primer, Index 1 (100% over 24bp)
AAATCCGGGATCTTAAGGCCGAGAGCTGATGACGAGTTACCTTGGAATTC132980.192746929282546No Hit
TCCCCATGTGAGAGTAGGTCATCGTCAAGATTGGAATTCTCGGGTGCCA132820.19251501840357768No Hit
CTTGCCTCAAGATGAGATCTCACTTGGAATTCTCGGGTGCCAAGGAACTC132410.19192074677622137RNA PCR Primer, Index 1 (100% over 26bp)
TGTTCTGTGACTGCGTACCTTTTGTATATGGAATTCTCGGGTGCCAAGG131720.19092063111067048Illumina Small RNA Adapter 2 (100% over 21bp)
GAAAAGTTAGCGGATGACCTGTGGATCGGTGGAATTCTCGGGTGCCAAGG129630.18789129525414677Illumina Small RNA Adapter 2 (100% over 21bp)
GGTGGTTGGGTAGAGAATACCAAGGCGCTTGAGAGTGGAATTCTCGGGTG111930.16223615426827623No Hit
TGAAAAGTTAGCGGATGACCTGTGGATCGGTGGAATTCTCGGGTGCCA110200.15972861788943127No Hit
ACTACTGACTGACCGATAGTGAACTTGGAATTCTCGGGTGCCAAGGAACT106820.15482950057122546RNA PCR Primer, Index 1 (100% over 25bp)
AGCAGGGTCGGGCCTGGTTAGTACTTGGATGGAATTCTCGGGTGCCAAGG104630.15165522041534657Illumina Small RNA Adapter 2 (100% over 21bp)
CCCATGTGAGAGTAGGTCATCGTCAAGATTGGAATTCTCGGGTGCCAAGG103720.15033622729121426Illumina Small RNA Adapter 2 (100% over 21bp)
TGTTCTGTGACTGCGTACCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT101920.14772722990282064RNA PCR Primer, Index 1 (100% over 30bp)
TCCTCCAAGGCTAAATACTACTGACTGACCGATAGTGAACTTGGAATTCT101260.14677059752707633No Hit
TATCTGCCGTGGACGTTTGAGATTTGAGAGGGGTGGAATTCTCGGGTGCC100760.14604587603030034No Hit
AACTTGCCTCAAGATGAGATCTCACTTGGAATTCTCGGGTGCCAAGGAAC100570.14577048186152544RNA PCR Primer, Index 1 (100% over 24bp)
AACACATGCAAGTCGAGCGGATGAGAGGAGCTTGGAATTCTCGGGTGCC98810.1432194621928739No Hit
AGTGACGAAAAATAACAATACAGGACTTGGAATTCTCGGGTGCCAAGGA97780.14172653590951534RNA PCR Primer, Index 1 (100% over 22bp)
CGGGAAACTTGCCTCAAGATGAGATCTCACTTGGAATTCTCGGGTGCCA95610.1385812446135075No Hit
AAGCGGGAAACTTGCCTCAAGATGAGATCTCACTTGGAATTCTCGGGTGC93960.13618966367414667No Hit
CGACCACCTGGACTGATACTGACACTGAGGTGCGTGGAATTCTCGGGTGC93820.13598674165504937No Hit
TCTGTGACTGCGTACCTTTTGTATATGGAATTCTCGGGTGCCAAGGAACT93530.1355664031869193RNA PCR Primer, Index 1 (100% over 25bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC88710.12858008795799863No Hit
AGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGATGGAATTCTCGGGTGC87270.12649289004728373No Hit
TTCCCCATGTGAGAGTAGGTCATCGTCAAGATTGGAATTCTCGGGTGCC86940.12601457385941156No Hit
TCCCCATGTGAGAGTAGGTCATCGTCAAGTGGAATTCTCGGGTGCCAAGG85780.12433321998689124Illumina Small RNA Adapter 2 (100% over 21bp)
TGCCTCAAGATGAGATCTCACTTGGAATTCTCGGGTGCCAAGGAACTCC85200.12349254305063107RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC84920.1230866990124365No Hit
TGTCAACTAGCCGTTGGGAGCCTGGAATTCTCGGGTGCCAAGGAACTCC83780.12143433399978722RNA PCR Primer, Index 1 (100% over 27bp)
TAAATCAGTTATGGTTCCTTTGGTCGCTCGCTCTGGAATTCTCGGGTGCC83050.12037624061449424No Hit
TGACGAAAAATAACAATACAGGACTTGGAATTCTCGGGTGCCAAGGAACT81840.11862241459229632RNA PCR Primer, Index 1 (100% over 25bp)
TGGCTCAGATTGAACGCTGGCGGCAGGCCTGGAATTCTCGGGTGCCAAGG81100.11754982677706784Illumina Small RNA Adapter 2 (100% over 21bp)
GGGTCGGGGGTCGGCGCGCGGCGCGGGCTGGAATTCTCGGGTGCCAAGG79530.11527420127719118Illumina Small RNA Adapter 2 (100% over 21bp)
CACAGGTGGTTGGGTAGAGAATACCAAGGCGCTGGAATTCTCGGGTGCC79380.11505678482815837No Hit
AATACTACTGACTGACCGATAGTGAACTTGGAATTCTCGGGTGCCAAGG79290.1149263349587387Illumina Small RNA Adapter 2 (100% over 21bp)
AAATACTACTGACTGACCGATAGTGAACTTGGAATTCTCGGGTGCCAAGG78660.11401318587280095Illumina Small RNA Adapter 2 (100% over 21bp)
TTGAGAGAACTCGGGTGAAGGAACTAGGTGGAATTCTCGGGTGCCAAGG77690.1126072261690555Illumina Small RNA Adapter 2 (100% over 21bp)
ACACATGCAAGTCGAGCGGATGAGAGGAGCTTGGAATTCTCGGGTGCCA77410.11220138213086092No Hit
CTAAATACTACTGACTGACCGATAGTGAACTTGGAATTCTCGGGTGCCA76280.11056351154814716No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCC75970.11011418422014604No Hit
TTGCCTCAAGATGAGATCTCACTTGGAATTCTCGGGTGCCAAGGAACTCC75920.11004171207046844RNA PCR Primer, Index 1 (100% over 27bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTTGGAATTCTCGG74890.10854878578710989No Hit
ACGAAAAATAACAATACAGGACTTGGAATTCTCGGGTGCCAAGGAACTCC74190.10753417569162346RNA PCR Primer, Index 1 (100% over 27bp)
TTGTTCTGTGACTGCGTACCTTTTGTATATGGAATTCTCGGGTGCCAAGG73580.10665001546555673Illumina Small RNA Adapter 2 (100% over 21bp)
AACACATGCAAGTCGAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT72610.10524405576181128RNA PCR Primer, Index 1 (100% over 30bp)
GAGCGGTCGGCGTCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG71120.1030843857014188RNA PCR Primer, Index 6 (100% over 34bp)
ATACAGAGGGTGCAAGCGTTAATCGGAATTGGAATTCTCGGGTGCCAAGG69700.10102617665057494Illumina Small RNA Adapter 2 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)