FastQCFastQC Report
Thu 28 Sept 2023
SRR6053481_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6053481_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3216002
Total Bases159.4 Mbp
Sequences flagged as poor quality0
Sequence length36-50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATCCGGGATCTTAAGGCCGAGAGCTGATGACGAGTTACCTTGGAATTC523421.627548739086605No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTTGGAATTCTCGG300680.9349496673198586No Hit
AGCGGGAAACTTGCCTCAAGATGAGATCTCACTTGGAATTCTCGGGTGCC290830.9043215769144421No Hit
CCCCATGTGAGAGTAGGTCATCGTCAAGATTGGAATTCTCGGGTGCCA283630.8819335311358638No Hit
GGTGGTTGGGTAGAGAATACCAAGGCGCTGGAATTCTCGGGTGCCAAGG269630.8384012198997389Illumina Small RNA Adapter 2 (100% over 21bp)
TTGAGAGAACTCGGGTGAAGGAACTTGGAATTCTCGGGTGCCAAGGAACT173030.5380282723704775RNA PCR Primer, Index 1 (100% over 25bp)
TGAGATTTGAGAGGGGCTGCTCCTTGGAATTCTCGGGTGCCAAGGAACTC147470.45855070985652374RNA PCR Primer, Index 1 (100% over 26bp)
CAGATAACCAGGAACCGTACCCCAAACCGACTGGAATTCTCGGGTGCCA146140.4544151402890918No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGGAATTCTCG129050.4012746260729937No Hit
CAGATAACCAGGAACCGTACCCCAAACCGACATGGAATTCTCGGGTGCC128510.39959552263960035No Hit
TCCTCCAAGGCTAAATACTACTGACTGACCGATAGTGAACTTGGAATTCT126520.39340771554246545No Hit
ATACTACTGACTGACCGATAGTGAACTTGGAATTCTCGGGTGCCAAGGA125260.3894898075312142RNA PCR Primer, Index 1 (100% over 22bp)
TCCCCATGTGAGAGTAGGTCATCGTCAAGATTGGAATTCTCGGGTGCCA123760.3848256313273437No Hit
AACTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTGG119780.3724500171330739No Hit
CTCAGATTGAACGCTGGCGGCAGGCCTGGAATTCTCGGGTGCCAAGGAAC112680.35037291643475343RNA PCR Primer, Index 1 (100% over 24bp)
CCAAGTTTAAGGTGGTAGGCTGAGATCTTAGGTTGGAATTCTCGGGTGCC112300.3491913251297729No Hit
TTCTGTGACTGCGTACCTTTTGTATATGGAATTCTCGGGTGCCAAGGAAC111310.34611296883521836RNA PCR Primer, Index 1 (100% over 24bp)
ACAGGTGGTTGGGTAGAGAATACCAAGGCGCTGGAATTCTCGGGTGCCA103420.32157940200285945No Hit
AGCAGGGTCGGGCCTGGTTAGTACTTGGATGGAATTCTCGGGTGCCAAGG102920.32002467660156925Illumina Small RNA Adapter 2 (100% over 21bp)
AGGCCGAGAGCTGATGACGAGTTACCTTGGAATTCTCGGGTGCCAAGGA101560.31579582351006RNA PCR Primer, Index 1 (100% over 22bp)
ATCTGCCGTGGACGTTTGAGATTTGAGAGGGGTGGAATTCTCGGGTGCC100910.3137746804883828No Hit
CCCCATGTGAGAGTAGGTCATCGTCAAGTGGAATTCTCGGGTGCCAAGG99340.3088928427283316Illumina Small RNA Adapter 2 (100% over 21bp)
CGGGGCTCAAACCATACACCGAAGCTACGGGTGGAATTCTCGGGTGCCA97280.30248737407501614No Hit
AGGTGGTTGGGTAGAGAATACCAAGGCGCTGGAATTCTCGGGTGCCAAGG96770.3009015541657002Illumina Small RNA Adapter 2 (100% over 21bp)
AGCGGGAAACTTGCCTCAAGATGAGATCTCACTGGAACCTGGAATTCTCG96310.2994712067965132No Hit
TACTACTGACTGACCGATAGTGAACTTGGAATTCTCGGGTGCCAAGGAAC93970.29219509191847515RNA PCR Primer, Index 1 (100% over 24bp)
TCCCCATGTGAGAGTAGGTCATCGTCAAGTGGAATTCTCGGGTGCCAAGG93820.29172867429808813Illumina Small RNA Adapter 2 (100% over 21bp)
AAAAGTTAGCGGATGACCTGTGGATCGGTGGAATTCTCGGGTGCCAAGG93110.2895209642282561Illumina Small RNA Adapter 2 (100% over 21bp)
AAGTTAGCGGATGACCTGTGGATCGGTGGAATTCTCGGGTGCCAAGGAAC93060.28936549168812703RNA PCR Primer, Index 1 (100% over 24bp)
CTTGCCTCAAGATGAGATCTCACTTGGAATTCTCGGGTGCCAAGGAACTC91030.28305330655888894RNA PCR Primer, Index 1 (100% over 26bp)
ACTTGCCTCAAGATGAGATCTCACTTGGAATTCTCGGGTGCCAAGGAACT88110.2739737102153543RNA PCR Primer, Index 1 (100% over 25bp)
AAAGTTAGCGGATGACCTGTGGATCGGTGGAATTCTCGGGTGCCAAGGA87270.27136177154118685RNA PCR Primer, Index 1 (100% over 22bp)
CAGATAACCAGGAACCGTACCCCAAACCGACACATGGAATTCTCGGGTGC86490.2689363999151742No Hit
TAGCGGATGACCTGTGGATCGGTGGAATTCTCGGGTGCCAAGGAACTCC84930.2640856566631488RNA PCR Primer, Index 1 (100% over 27bp)
TCTAAGCGGGAAACTTGCCTCAAGATGAGATCTCACTTGGAATTCTCGGG84200.2618157575772652No Hit
AAGCAGGGTCGGGCCTGGTTAGTACTTGGATGGAATTCTCGGGTGCCA83660.2601366541438718No Hit
TAACACATGCAAGTCGAGCGGATGAGAGGAGCTTGGAATTCTCGGGTGCC83570.2598568035716396No Hit
CAGATAACCAGGAACCGTACCCCAAACCGATGGAATTCTCGGGTGCCA82280.25584561203631095No Hit
TGCCTCAAGATGAGATCTCACTGGAACCTGGAATTCTCGGGTGCCAAGG81600.2537311854905563Illumina Small RNA Adapter 2 (100% over 21bp)
ACTACTGACTGACCGATAGTGAACTTGGAATTCTCGGGTGCCAAGGAACT80670.2508393962441566RNA PCR Primer, Index 1 (100% over 25bp)
AATACCAAGGCGCTTGAGAGAACTCGGGTGAAGGAACTTGGAATTCTCGG79280.24651725962856985No Hit
AAGGCCGAGAGCTGATGACGAGTTACCTTGGAATTCTCGGGTGCCAAGG77340.24048492507156402Illumina Small RNA Adapter 2 (100% over 21bp)
AGTTAGCGGATGACCTGTGGATCGGTGGAATTCTCGGGTGCCAAGGAACT75890.23597622140782254RNA PCR Primer, Index 1 (100% over 25bp)
CAGGTGGTTGGGTAGAGAATACCAAGGCGCTGGAATTCTCGGGTGCCA75580.2350122916590226No Hit
TGTTCTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTGGAATTCT72200.22450234794630103No Hit
TCTGCCGTGGACGTTTGAGATTTGAGAGGGGTGGAATTCTCGGGTGCCA67140.20876852688524447No Hit
AAATCCGGGATCTTAAGGCCGAGAGCTGATTGGAATTCTCGGGTGCCA65960.20509937493819966No Hit
TTGAGAGAACTCGGGTGAAGGAACTAGGCAAAATGGCACCGTGGAATTCT63910.19872500079290994No Hit
AAACTTTGTCTGAATATGGGGGGACCTGGAATTCTCGGGTGCCAAGGAAC63820.19844515022067774RNA PCR Primer, Index 1 (100% over 24bp)
AGCAGGGTCGGGCCTGGTTAGTACTTGGTGGAATTCTCGGGTGCCAAGG62170.19331455639642015Illumina Small RNA Adapter 2 (100% over 21bp)
CAGATAACCAGGAACCGTACCCCAAACCGACACTGGAATTCTCGGGTGCC61260.19048495616607206No Hit
GTTCTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTGGAATTCTC59760.18582077996220153No Hit
CGGGAAACTTGCCTCAAGATGAGATCTCACTTGGAATTCTCGGGTGCCA58610.1822449115392341No Hit
TGTTCTGTGACTGCGTACCTTTTGTATATGGAATTCTCGGGTGCCAAGG58590.18218272252318252Illumina Small RNA Adapter 2 (100% over 21bp)
GGTGGTTGGGTAGAGAATACCAAGGCGCTTGAGAGTGGAATTCTCGGGTG58330.18137426531451162No Hit
TCATGGCTCAGATTGAACGCTGGCGGCAGGCCTGGAATTCTCGGGTGCC57780.17966406737309243No Hit
TATCTGCCGTGGACGTTTGAGATTTGAGAGGGGTGGAATTCTCGGGTGCC54430.16924740718444825No Hit
AACTTTGTCTGAATATGGGGGGACCTGGAATTCTCGGGTGCCAAGGAACT52370.16284193853113277RNA PCR Primer, Index 1 (100% over 25bp)
TGGCTCAGATTGAACGCTGGCGGCAGGCCTGGAATTCTCGGGTGCCAAGG52130.16209567033851346Illumina Small RNA Adapter 2 (100% over 21bp)
GAAAAGTTAGCGGATGACCTGTGGATCGGTGGAATTCTCGGGTGCCAAGG51710.16078970100142972Illumina Small RNA Adapter 2 (100% over 21bp)
TGGCTTTGAGATTAGCGGAACGCTCTGGAAAGTGCGGCCATAGTTGGAAT49940.1552859730808625No Hit
AGAGCGTTGGAACCACCTGATCCCATCCCGAACTCAGATGGAATTCTCGG48060.14944020557201146No Hit
TTCGGTCCCTATCTGCCGTGGACGTTTGAGATTTGAGAGGGGTGGAATTC47150.14661060534166334No Hit
TTCTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTGGAATTCTCG46640.14502478543234737No Hit
CCTCCAAGGCTAAATACTACTGACTGACCGATAGTGAACTTGGAATTCTC46610.14493150190826998No Hit
CGACCACCTGGACTGATACTGACACTGAGGTGCGTGGAATTCTCGGGTGC46170.14356334355513461No Hit
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC44700.13899245087534148RNA PCR Primer, Index 2 (100% over 34bp)
TTCCCCATGTGAGAGTAGGTCATCGTCAAGATTGGAATTCTCGGGTGCC44310.13777976506233516No Hit
TCTGTGACTGCGTACCTTTTGTATATGGAATTCTCGGGTGCCAAGGAACT44120.1371889694098449RNA PCR Primer, Index 1 (100% over 25bp)
AATACTACTGACTGACCGATAGTGAACTTGGAATTCTCGGGTGCCAAGG44090.13709568588576748Illumina Small RNA Adapter 2 (100% over 21bp)
TCGGTCCCTATCTGCCGTGGACGTTTGAGATTTGAGAGGGGTGGAATTCT43570.13547877146842568No Hit
CCCATGTGAGAGTAGGTCATCGTCAAGATTGGAATTCTCGGGTGCCAAGG43550.1354165824523741Illumina Small RNA Adapter 2 (100% over 21bp)
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCTGGAATTCTCGGGT42960.13358200647885168No Hit
ACACATGCAAGTCGAGCGGATGAGAGGAGCTTGGAATTCTCGGGTGCCA42640.132586982222026No Hit
ATACCAAGGCGCTTGAGAGAACTCGGGTGAAGGAACTTGGAATTCTCGGG41200.1281093730663103No Hit
TGAAAAGTTAGCGGATGACCTGTGGATCGGTGGAATTCTCGGGTGCCA40720.12661683668107174No Hit
TCTTGACGACCATAGAGCGTTGGAACCACCTGATTGGAATTCTCGGGTGC40110.1247200716914977No Hit
GATGTCAACTAGCCGTTGGGAGCCTGGAATTCTCGGGTGCCAAGGAACTC39840.12388051997480101RNA PCR Primer, Index 1 (100% over 26bp)
TTGAGAGAACTCGGGTGAAGGAACTAGGCAAAATGGCACCGTTGGAATTC39470.12273002317784629No Hit
AGTGGCATTGCCGGGTAGCTATGTTCGGGATGGAATTCTCGGGTGCCA39360.12238798358956245No Hit
AACTTGCCTCAAGATGAGATCTCACTTGGAATTCTCGGGTGCCAAGGAAC38490.11968276139131755RNA PCR Primer, Index 1 (100% over 24bp)
CTCAGATTGAACGCTGGCGGCAGGCCTTGGAATTCTCGGGTGCCAAGGA38130.11856335910238863RNA PCR Primer, Index 1 (100% over 22bp)
AACACATGCAAGTCGAGCGGATGAGAGGAGCTTGGAATTCTCGGGTGCC37990.11812803599002737No Hit
ACGATGTCAACTAGCCGTTGGGAGCCTGGAATTCTCGGGTGCCAAGGAAC37680.11716410624122746RNA PCR Primer, Index 1 (100% over 24bp)
AAGGCGCTTGAGAGAACTCGGGTGAAGGAACTTGGAATTCTCGGGTGCC36240.11268649708551176No Hit
GATAGGTTGGGTGTGTAAGCGCTTGGAATTCTCGGGTGCCAAGGAACTCC36170.11246883552933115RNA PCR Primer, Index 1 (100% over 27bp)
CTTGCCTCAAGATGAGATCTCACTGGAACCTGGAATTCTCGGGTGCCA35630.11078973209593776No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC34890.1084887385020283No Hit
AGCATCTAAGCGGGAAACTTGCCTCAAGATGAGATCTCACTTGGAATTCT34810.10823998243782186No Hit
AAATCCGGGATCTTAAGGCCGAGAGCTGATGACGAGTTACCTTTGGAATT34580.1075248087532284No Hit
TCCGGATCGAGGACAGTGTATGGTGGGTAGTTGGAATTCTCGGGTGCCA34490.10724495818099616No Hit
TTCTGTGACTGCGTACCTTTTGTATGGAATTCTCGGGTGCCAAGGAACTC34150.10618774490811884RNA PCR Primer, Index 1 (100% over 26bp)
GAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTGGAATT33970.10562804376365437No Hit
CGGGAAACTTGCCTCAAGATGAGATCTCACTGGAACCTGGAATTCTCGGG33250.10338923918579654No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC32290.1004041664153194No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)