FastQCFastQC Report
Thu 28 Sept 2023
SRR6053482_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6053482_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2404665
Total Bases119.3 Mbp
Sequences flagged as poor quality0
Sequence length40-50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTTGGAATTCTCGG544532.264473429770883No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGGAATTCTCG272321.1324654369735494No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGA183470.7629753000937761No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTGGAATTCTCGGG169730.7058363639010008No Hit
AGCAGGGTCGGGCCTGGTTAGTACTTGGATGGAATTCTCGGGTGCCAAGG125640.5224844209068623Illumina Small RNA Adapter 2 (100% over 21bp)
AAGCAGGGTCGGGCCTGGTTAGTACTTGGATGGAATTCTCGGGTGCCA106060.4410593575404474No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC92400.3842531080212836No Hit
AACGTCCGTCGTGAAAAGGGAAACAACCCAGACCGTCTGGAATTCTCGGG89880.37377347780252135No Hit
CGTCCGTCGTGAAAAGGGAAACAACCCAGACCGTCTGGAATTCTCGGGTG82220.34191872880422014No Hit
AAATCCGGGATCTTAAGGCCGAGAGCTGATGACGAGTTACCTTGGAATTC75370.31343243237623536No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTTGGAATTCTCGGGTG72960.30341024633368885No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCC72190.3002081371001782No Hit
GCAGGGTCGGGCCTGGTTAGTACTTGGATGGAATTCTCGGGTGCCAAGG70360.2925979294413151Illumina Small RNA Adapter 2 (100% over 21bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGC70230.29205731359669646No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCTGGAATTCTCGGGT66310.2757556665897329No Hit
AGCGGGAAACTTGCCTCAAGATGAGATCTCACTTGGAATTCTCGGGTGCC66150.2750902932425099No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC61270.25479640615220833No Hit
CCCCATGTGAGAGTAGGTCATCGTCAAGATTGGAATTCTCGGGTGCCA56860.23645705326937433No Hit
ACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAAT54990.22868050227370545No Hit
CAGATAACCAGGAACCGTACCCCAAACCGACTGGAATTCTCGGGTGCCA54290.22576949387960485No Hit
CGGGGCTCAAACCATACACCGAAGCTACGGGTGGAATTCTCGGGTGCCA53270.2215277387910582No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCA52410.21795135704973456No Hit
AGCAGGGTCGGGCCTGGTTAGTACTTGGTGGAATTCTCGGGTGCCAAGG49320.20510133428149036Illumina Small RNA Adapter 2 (100% over 21bp)
GGTGGTTGGGTAGAGAATACCAAGGCGCTGGAATTCTCGGGTGCCAAGG47270.19657623827019563Illumina Small RNA Adapter 2 (100% over 21bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCA46010.1913364231608145No Hit
TTGAGAGAACTCGGGTGAAGGAACTTGGAATTCTCGGGTGCCAAGGAACT44110.18343511466254137RNA PCR Primer, Index 1 (100% over 25bp)
CAGATAACCAGGAACCGTACCCCAAACCGACATGGAATTCTCGGGTGCC40120.16684236681616776No Hit
AATACCAAGGCGCTTGAGAGAACTCGGGTGAAGGAACTTGGAATTCTCGG39130.1627253692302254No Hit
CTCAGATTGAACGCTGGCGGCAGGCCTGGAATTCTCGGGTGCCAAGGAAC37940.15777665496025434RNA PCR Primer, Index 1 (100% over 24bp)
TCATGGCTCAGATTGAACGCTGGCGGCAGGCCTGGAATTCTCGGGTGCC35570.14792081225451362No Hit
TCTTGACGACCATAGAGCGTTGGAACCACCTTGGAATTCTCGGGTGCCA34800.14471870302100293No Hit
GGCGGCGCTGTCTCCACCCGAGACTCAGTGGAATTCTCGGGTGCCAAGG34670.14417808717638425Illumina Small RNA Adapter 2 (100% over 21bp)
AACTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTGG34270.14251465380832673No Hit
AGCGGAACGCTCTGGAAAGTGCGGCCTGGAATTCTCGGGTGCCAAGGAAC34080.1417245229584994RNA PCR Primer, Index 1 (100% over 24bp)
TCCGTCGTGAAAAGGGAAACAACCCAGACCGTCTGGAATTCTCGGGTGCC33970.14126707878228362No Hit
TCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCTCGGGTGCCA33330.1386055853933916No Hit
ACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTTGGA31750.13203502358956445No Hit
TGGCGGCGCTGTCTCCACCCGAGACTCAGTTGGAATTCTCGGGTGCCA31720.13191026608696013No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAT31660.13166075108175151No Hit
ATACTACTGACTGACCGATAGTGAACTTGGAATTCTCGGGTGCCAAGGA31650.13161916524755007RNA PCR Primer, Index 1 (100% over 22bp)
GGCGGCGCTGTCTCCACCCGAGACTCAGTTGGAATTCTCGGGTGCCAAGG31430.13070427689511843Illumina Small RNA Adapter 2 (100% over 21bp)
AAGCAGGGTCGGGCCTGGTTAGTACTTGGTGGAATTCTCGGGTGCCAAGG30550.12704472348539195Illumina Small RNA Adapter 2 (100% over 21bp)
TCTAAGCGGGAAACTTGCCTCAAGATGAGATCTCACTTGGAATTCTCGGG30540.1270031376511905No Hit
TGGCGGCGCTGTCTCCACCCGAGACTCAGTGGAATTCTCGGGTGCCAAGG30110.1252149467805287Illumina Small RNA Adapter 2 (100% over 21bp)
AAGTTAGCGGATGACCTGTGGATCGGTGGAATTCTCGGGTGCCAAGGAAC29630.12321882673885967RNA PCR Primer, Index 1 (100% over 24bp)
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGC28680.11926817248972309No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCA28620.11901865748451448No Hit
ACCAAGGCGCTTGAGAGAACTCGGGTGAAGGAACTTGGAATTCTCGGGTG28450.11831169830309003No Hit
TACCAAGGCGCTTGAGAGAACTCGGGTGAAGGAACTTGGAATTCTCGGGT27630.11490165989857216No Hit
AAGGCGCTTGAGAGAACTCGGGTGAAGGAACTTGGAATTCTCGGGTGCC27470.11423628655134914No Hit
CTGATCTCGGAAGCTAAGCAGGGTCGGGCCTGGTTTGGAATTCTCGGGTG26800.11145003565985283No Hit
ATACCAAGGCGCTTGAGAGAACTCGGGTGAAGGAACTTGGAATTCTCGGG26700.11103417731783845No Hit
AAAAGTTAGCGGATGACCTGTGGATCGGTGGAATTCTCGGGTGCCAAGG25720.10695876556609757Illumina Small RNA Adapter 2 (100% over 21bp)
TCCTCCAAGGCTAAATACTACTGACTGACCGATAGTGAACTTGGAATTCT25490.10600229137946451No Hit
AAAGTTAGCGGATGACCTGTGGATCGGTGGAATTCTCGGGTGCCAAGGA25390.10558643303745013RNA PCR Primer, Index 1 (100% over 22bp)
AACACATGCAAGTCGAGCGGATGAGAGGAGCTTGGAATTCTCGGGTGCC25150.10458837301661561No Hit
AGTTAGCGGATGACCTGTGGATCGGTGGAATTCTCGGGTGCCAAGGAACT25120.1044636155140113RNA PCR Primer, Index 1 (100% over 25bp)
GCAGGGTCGGGCCTGGTTAGTACTTGGTGGAATTCTCGGGTGCCAAGGA25020.10404775717199692RNA PCR Primer, Index 1 (100% over 22bp)
CAGATAACCAGGAACCGTACCCCAAACCGACACATGGAATTCTCGGGTGC24900.10354872716157967No Hit
AGCAGGGTCGGGCCTGGTTAGTACTTGGATGGGTGGAATTCTCGGGTGCC24870.10342396965897536No Hit
AAGCAGGGTCGGGCCTGGTTAGTACTTGGATGGGTGGAATTCTCGGGTGC24650.10250908130654375No Hit
TGGCTCAGATTGAACGCTGGCGGCAGGCCTGGAATTCTCGGGTGCCAAGG24210.1006793046016805Illumina Small RNA Adapter 2 (100% over 21bp)
AAGGCGCTTGAGAGAACTCGGGTGAAGGAACTAGGCTGGAATTCTCGGGT24140.10038820376227042No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)