FastQCFastQC Report
Thu 28 Sept 2023
SRR6053483_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6053483_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2772996
Total Bases137.6 Mbp
Sequences flagged as poor quality0
Sequence length45-50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATCCGGGATCTTAAGGCCGAGAGCTGATGACGAGTTACCTTGGAATTC288801.0414728329936285No Hit
CCCCATGTGAGAGTAGGTCATCGTCAAGATTGGAATTCTCGGGTGCCA193020.6960702431593844No Hit
GGTGGTTGGGTAGAGAATACCAAGGCGCTGGAATTCTCGGGTGCCAAGG186060.6709710363808675Illumina Small RNA Adapter 2 (100% over 21bp)
AACTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTGG131780.47522607317140014No Hit
TCCTCCAAGGCTAAATACTACTGACTGACCGATAGTGAACTTGGAATTCT131480.4741442108102572No Hit
AGCGGGAAACTTGCCTCAAGATGAGATCTCACTTGGAATTCTCGGGTGCC128990.46516475321277057No Hit
TTGAGAGAACTCGGGTGAAGGAACTTGGAATTCTCGGGTGCCAAGGAACT112130.4043640885165359RNA PCR Primer, Index 1 (100% over 25bp)
CAGATAACCAGGAACCGTACCCCAAACCGACTGGAATTCTCGGGTGCCA108770.3922472300717347No Hit
CAGATAACCAGGAACCGTACCCCAAACCGACATGGAATTCTCGGGTGCC102550.3698166171173705No Hit
ATACTACTGACTGACCGATAGTGAACTTGGAATTCTCGGGTGCCAAGGA98450.3550311648484167RNA PCR Primer, Index 1 (100% over 22bp)
GAACTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTG84700.30544580662936405No Hit
CTCAGATTGAACGCTGGCGGCAGGCCTGGAATTCTCGGGTGCCAAGGAAC81670.2945189967818201RNA PCR Primer, Index 1 (100% over 24bp)
CAGATAACCAGGAACCGTACCCCAAACCGACACATGGAATTCTCGGGTGC79390.2862968428371335No Hit
TCTAAGCGGGAAACTTGCCTCAAGATGAGATCTCACTTGGAATTCTCGGG71910.25932240796596895No Hit
TACTACTGACTGACCGATAGTGAACTTGGAATTCTCGGGTGCCAAGGAAC70760.2551752689149209RNA PCR Primer, Index 1 (100% over 24bp)
ACAGGTGGTTGGGTAGAGAATACCAAGGCGCTGGAATTCTCGGGTGCCA68660.24760223238692014No Hit
CCAAGTTTAAGGTGGTAGGCTGAGATCTTAGGTTGGAATTCTCGGGTGCC67370.2429502242340054No Hit
TCTGAATGGGGGAACCCAGCCATCACAAGATGGTTATCTTGTACTTGGA66950.24143561692840523No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTTGGAATTCTCGG66800.24089468574783374No Hit
AGGTGGTTGGGTAGAGAATACCAAGGCGCTGGAATTCTCGGGTGCCAAGG63920.23050880708086127Illumina Small RNA Adapter 2 (100% over 21bp)
AAGTTAGCGGATGACCTGTGGATCGGTGGAATTCTCGGGTGCCAAGGAAC63750.2298957517428803RNA PCR Primer, Index 1 (100% over 24bp)
CAGATAACCAGGAACCGTACCCCAAACCGATGGAATTCTCGGGTGCCA60650.21871650734440296No Hit
TGAGATTTGAGAGGGGCTGCTCCTTGGAATTCTCGGGTGCCAAGGAACTC60030.21648065846470746RNA PCR Primer, Index 1 (100% over 26bp)
AGGCCGAGAGCTGATGACGAGTTACCTTGGAATTCTCGGGTGCCAAGGA59210.2135235680109167RNA PCR Primer, Index 1 (100% over 22bp)
AAAAGTTAGCGGATGACCTGTGGATCGGTGGAATTCTCGGGTGCCAAGG58970.21265807812200235Illumina Small RNA Adapter 2 (100% over 21bp)
TTCTGTGACTGCGTACCTTTTGTATATGGAATTCTCGGGTGCCAAGGAAC58840.2121892710988404RNA PCR Primer, Index 1 (100% over 24bp)
AAATCCGGGATCTTAAGGCCGAGAGCTGATTGGAATTCTCGGGTGCCA56790.20479654496436345No Hit
AAACTTTGTCTGAATATGGGGGGACCTGGAATTCTCGGGTGCCAAGGAAC55680.2007936542281345RNA PCR Primer, Index 1 (100% over 24bp)
AAGGCCGAGAGCTGATGACGAGTTACCTTGGAATTCTCGGGTGCCAAGG55590.20046909551979158Illumina Small RNA Adapter 2 (100% over 21bp)
AAAGTTAGCGGATGACCTGTGGATCGGTGGAATTCTCGGGTGCCAAGGA54620.19697107388542934RNA PCR Primer, Index 1 (100% over 22bp)
ATCTGCCGTGGACGTTTGAGATTTGAGAGGGGTGGAATTCTCGGGTGCC54510.1965743910196769No Hit
GTTCTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTGGAATTCTC54050.19491553539925768No Hit
TAACACATGCAAGTCGAGCGGATGAGAGGAGCTTGGAATTCTCGGGTGCC53380.1924993761260384No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTGGAATTCTCGGG52850.19058808595468585No Hit
TGGCGGCGCTGTCTCCACCCGAGACTCAGTGAAATTGAAATCGCTGTGA52360.1888210440981523No Hit
TCCCCATGTGAGAGTAGGTCATCGTCAAGATTGGAATTCTCGGGTGCCA52290.18856860954721896No Hit
CAGATAACCAGGAACCGTACCCCAAACCGACACTGGAATTCTCGGGTGCC51350.185178774148971No Hit
ACTACTGACTGACCGATAGTGAACTTGGAATTCTCGGGTGCCAAGGAACT50400.18175287667201828RNA PCR Primer, Index 1 (100% over 25bp)
CAGGTGGTTGGGTAGAGAATACCAAGGCGCTGGAATTCTCGGGTGCCA49040.17684843396817015No Hit
CGGGGCTCAAACCATACACCGAAGCTACGGGTGGAATTCTCGGGTGCCA48530.1750092679542271No Hit
GGTGGTTGGGTAGAGAATACCAAGGCGCTTGAGAGTGGAATTCTCGGGTG48480.1748289575607033No Hit
TGTTCTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTGGAATTCT48090.17342253649121744No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAT47350.17075394266706478No Hit
TAAGTGGCTTTGAGATTAGCGGAACGCTCTGGAAAGTGCGGCCATAGTTG47190.17017694940778855No Hit
ACTTGCCTCAAGATGAGATCTCACTTGGAATTCTCGGGTGCCAAGGAACT44690.16116142973159717RNA PCR Primer, Index 1 (100% over 25bp)
AGTTAGCGGATGACCTGTGGATCGGTGGAATTCTCGGGTGCCAAGGAACT44340.15989925697693036RNA PCR Primer, Index 1 (100% over 25bp)
CCCCATGTGAGAGTAGGTCATCGTCAAGTGGAATTCTCGGGTGCCAAGG43780.1578797805694635Illumina Small RNA Adapter 2 (100% over 21bp)
TCCTACTTGGATAACTGTGGTAATTCTAGTGGAATTCTCGGGTGCCAAGG43470.15676185612961577Illumina Small RNA Adapter 2 (100% over 21bp)
CCTCCAAGGCTAAATACTACTGACTGACCGATAGTGAACTTGGAATTCTC42340.15268684123597726No Hit
TGTTCTGTGACTGCGTACCTTTTGTATATGGAATTCTCGGGTGCCAAGG42320.15261471707856772Illumina Small RNA Adapter 2 (100% over 21bp)
ACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAAT41830.1508476752220342No Hit
ACTCTGCAATTTTGACGTTACCGACAGAATAAGCACCGGCTAACTCTGTT41730.15048705443498656No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCGCTCTGGAATTCTCGGGTGCC40550.14623172914782423No Hit
TCATGGCTCAGATTGAACGCTGGCGGCAGGCCTGGAATTCTCGGGTGCC40000.14424831481906214No Hit
AATACCAAGGCGCTTGAGAGAACTCGGGTGAAGGAACTTGGAATTCTCGG39580.14273370751346198No Hit
ACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTTGGA38880.14020936200412837No Hit
TTGAGAGAACTCGGGTGAAGGAACTAGGCAAAATGGCACCGTGGAATTCT38090.13736045778645192No Hit
TCTGCCGTGGACGTTTGAGATTTGAGAGGGGTGGAATTCTCGGGTGCCA37500.13523279514287076No Hit
GGCGGCGCTGTCTCCACCCGAGACTCAGTGAAATTGAAATCGCTGTGAAT36840.13285269794835622No Hit
AATACTACTGACTGACCGATAGTGAACTTGGAATTCTCGGGTGCCAAGG36540.13177083558721325Illumina Small RNA Adapter 2 (100% over 21bp)
AAAACCGATCGTAGTCCGGATCGCAGTCTGCAACTCGACTGCGTGAAGTC36450.13144627687887037No Hit
CCCATGTGAGAGTAGGTCATCGTCAAGATTGGAATTCTCGGGTGCCAAGG36140.13032835243902263Illumina Small RNA Adapter 2 (100% over 21bp)
AACTTTGTCTGAATATGGGGGGACCTGGAATTCTCGGGTGCCAAGGAACT35950.1296431729436321RNA PCR Primer, Index 1 (100% over 25bp)
AAGTGGCTTTGAGATTAGCGGAACGCTCTGGAAAGTGCGGCCATAGTTGG35480.1279482552445081No Hit
AACTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTTG35130.1266860824898413No Hit
GAACTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTT34140.12311593669806953No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTTGGAATTCTCGGGTG33900.12225044680915516No Hit
TTGAGAGAACTCGGGTGAAGGAACTAGGCAAAATGGCACCGTTGGAATTC33750.12170951562858368No Hit
CGACCACCTGGACTGATACTGACACTGAGGTGCGTGGAATTCTCGGGTGC33710.12156526731376462No Hit
TAAATCAGTTATGGTTCCTTTGGTCGCTCGCTCTGGAATTCTCGGGTGCC33300.12008672208686923No Hit
AGATCCTGAAACCGTATGCGTACAAGTGGAATTCTCGGGTGCCAAGGAAC32980.11893273556831671RNA PCR Primer, Index 1 (100% over 24bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGA32250.11630020382286885No Hit
AAATACTACTGACTGACCGATAGTGAACTTGGAATTCTCGGGTGCCAAGG32250.11630020382286885Illumina Small RNA Adapter 2 (100% over 21bp)
CTTGCCTCAAGATGAGATCTCACTTGGAATTCTCGGGTGCCAAGGAACTC31820.11474953443856392RNA PCR Primer, Index 1 (100% over 26bp)
TGGCGGCGCTGTCTCCACCCGAGACTCAGTGAAATTGAAATCGCTGTGTG31520.11366767207742096No Hit
TGGCTCAGATTGAACGCTGGCGGCAGGCCTGGAATTCTCGGGTGCCAAGG30280.10919597431803002Illumina Small RNA Adapter 2 (100% over 21bp)
AGAGCGTTGGAACCACCTGATCCCATCCCGAACTCAGATGGAATTCTCGG30090.10851079482263948No Hit
AAATCCGGGATCTTAAGGCCGAGAGCTGATGACGAGTTACCTTTGGAATT29870.10771742909113464No Hit
AACTTGCCTCAAGATGAGATCTCACTTGGAATTCTCGGGTGCCAAGGAAC29700.10710437375315363RNA PCR Primer, Index 1 (100% over 24bp)
GAAAAGTTAGCGGATGACCTGTGGATCGGTGGAATTCTCGGGTGCCAAGG29290.10562582852625825Illumina Small RNA Adapter 2 (100% over 21bp)
AAAACACAGCACTCTGCAAACACGAAAGTGGACGTGGAATTCTCGGGTGC29140.10508489734568677No Hit
TATCTGCCGTGGACGTTTGAGATTTGAGAGGGGTGGAATTCTCGGGTGCC28970.10447184200770573No Hit
AACACATGCAAGTCGAGCGGATGAGAGGAGCTTGGAATTCTCGGGTGCC28530.10288511054469605No Hit
TTCTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTGGAATTCTCG28470.10266873807246747No Hit
CGGGAAACTTGCCTCAAGATGAGATCTCACTTGGAATTCTCGGGTGCCA28440.10256055183635318No Hit
TTCCCCATGTGAGAGTAGGTCATCGTCAAGATTGGAATTCTCGGGTGCC28380.10234417936412457No Hit
TAAGTGGCTTTGAGATTAGCGGAACGCTCTGGAAAGTGCGGCCATTGGA28370.1023081172854198No Hit
GGCGGCGCTGTCTCCACCCGAGACTCAGTGAAATTGAAATCGCTGTGTGG28120.10140656531780066No Hit
TGGCGGCGCTGTCTCCACCCGAGACTCAGTGAAATTGAAATCGCTGTGAT27870.10050501335018154No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)