FastQCFastQC Report
Thu 28 Sept 2023
SRR6053484_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6053484_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5962358
Total Bases295 Mbp
Sequences flagged as poor quality0
Sequence length38-50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCC351960.5903033665539708No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC277750.4658391864426792No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCA268730.4507109435562239No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTGGAATTCTCGGG239030.4008984364910661No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTTGGAATTCTCGGGTG207190.34749674541515285No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTTGGAATTCTCGG181980.3052148160174213No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCGCTCTGGAATTCTCGGGTGCC173010.2901704325704696No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGC151250.2536748044984887No Hit
TACATGCCGACGGGCGCTGACCCCCCTTGGAATTCTCGGGTGCCAAGGA148750.24948183252330708RNA PCR Primer, Index 1 (100% over 22bp)
TCCTACTTGGATAACTGTGGTAATTCTAGTGGAATTCTCGGGTGCCAAGG142460.23893231503375006Illumina Small RNA Adapter 2 (100% over 21bp)
CTCAGATTGAACGCTGGCGGCAGGCCTGGAATTCTCGGGTGCCAAGGAAC134390.22539740149786378RNA PCR Primer, Index 1 (100% over 24bp)
TGGCGGCGCTGTCTCCACCCGAGACTCAGTGGAATTCTCGGGTGCCAAGG132900.22289839020065552Illumina Small RNA Adapter 2 (100% over 21bp)
GTCAACTAGCCGTTGGGAGCCTGGAATTCTCGGGTGCCAAGGAACTCC120170.20154777690303063RNA PCR Primer, Index 1 (100% over 27bp)
GGCGGCGCTGTCTCCACCCGAGACTCAGTGGAATTCTCGGGTGCCAAGG115840.19428554944201606Illumina Small RNA Adapter 2 (100% over 21bp)
ACCACCCTGAACGCGCCCGATCTCGTGGAATTCTCGGGTGCCAAGGAACT109050.18289743755742274RNA PCR Primer, Index 1 (100% over 25bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCTGGAATTCTCGGGT107860.1809015828972363No Hit
TAAATCAGTTATGGTTCCTTTGGTCGCTCGCTCTGGAATTCTCGGGTGCC106960.17939211298617092No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCGCTTGGAATTCTCGGGTGCCA105460.17687632980106194No Hit
CATGCCGACGGGCGCTGACCCCCCTTGGAATTCTCGGGTGCCAAGGAACT99110.16622618098410058RNA PCR Primer, Index 1 (100% over 25bp)
TCTGATCTCGGAAGCTAAGCAGGGTCGGGCCTGGTGGAATTCTCGGGTGC98110.16454899219402794No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCGCTGGAATTCTCGGGTGCCA96890.16250282187013929No Hit
TCTTGACGACCATAGAGCGTTGGAACCACCTTGGAATTCTCGGGTGCCA90270.15139983207985833No Hit
CGGCGCTGTCTCCACCCGAGACTCAGTGGAATTCTCGGGTGCCAAGGAAC90250.15136628830405688RNA PCR Primer, Index 1 (100% over 24bp)
ACGAAAAATAACAATACAGGACTTGGAATTCTCGGGTGCCAAGGAACTCC84950.1424771877166718RNA PCR Primer, Index 1 (100% over 27bp)
TCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA82270.1379823217592771No Hit
TGACGAAAAATAACAATACAGGACTTGGAATTCTCGGGTGCCAAGGAACT82140.1377642872165677RNA PCR Primer, Index 1 (100% over 25bp)
GTCTACGGCCATACCACCCTGAACGTGGAATTCTCGGGTGCCAAGGAACT80680.13531559158306158RNA PCR Primer, Index 1 (100% over 25bp)
TGGCGGCGCTGTCTCCACCCGAGACTCAGTTGGAATTCTCGGGTGCCA78160.13108907583207852No Hit
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT77970.1307704099619647RNA PCR Primer, Index 1 (100% over 30bp)
ATGGCGGCGCTGTCTCCACCCGAGACTCAGTGGAATTCTCGGGTGCCA77880.1306194629708582No Hit
AGCAGGGTCGGGCCTGGTTAGTACTTGGATGGAATTCTCGGGTGCCAAGG76620.12850620509536664Illumina Small RNA Adapter 2 (100% over 21bp)
AGCGGATGACCTGTGGATCGGTGGAATTCTCGGGTGCCAAGGAACTCC75320.12632585966827217RNA PCR Primer, Index 1 (100% over 27bp)
AGATCCTGAAACCGTATGCGTACAAGTGGAATTCTCGGGTGCCAAGGAAC74100.12427968934438355RNA PCR Primer, Index 1 (100% over 24bp)
AGTGACGAAAAATAACAATACAGGACTTGGAATTCTCGGGTGCCAAGGA73760.12370944515575884RNA PCR Primer, Index 1 (100% over 22bp)
CCACCCTGAACGCGCCCGATCTCGTGGAATTCTCGGGTGCCAAGGAACTC73060.122535413002708RNA PCR Primer, Index 1 (100% over 26bp)
TGCCGACGGGCGCTGACCCCCCTTGGAATTCTCGGGTGCCAAGGAACTCC71980.12072404910942952RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC71370.11970096394748522No Hit
CGGGGCTCAAACCATACACCGAAGCTACGGGTGGAATTCTCGGGTGCCA69950.11731935586558205No Hit
CGAAAAATAACAATACAGGACTTGGAATTCTCGGGTGCCAAGGAACTCC69040.11579311406661594RNA PCR Primer, Index 1 (100% over 27bp)
TAGCGGATGACCTGTGGATCGGTGGAATTCTCGGGTGCCAAGGAACTCC68550.11497129155948033RNA PCR Primer, Index 1 (100% over 27bp)
AGCGGAACGCTCTGGAAAGTGCGGCCTGGAATTCTCGGGTGCCAAGGAAC68180.11435073170715344RNA PCR Primer, Index 1 (100% over 24bp)
GACGAAAAATAACAATACAGGACTTGGAATTCTCGGGTGCCAAGGAACTC67980.11401529394913891RNA PCR Primer, Index 1 (100% over 26bp)
TACCACCCTGAACGCGCCCGATCTCGTGGAATTCTCGGGTGCCAAGGAAC67840.11378048751852873RNA PCR Primer, Index 1 (100% over 24bp)
TACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC67310.11289157745979024RNA PCR Primer, Index 1 (100% over 31bp)
CAGATAACCAGGAACCGTACCCCAAACCGACTGGAATTCTCGGGTGCCA66820.11206975495265464No Hit
TGTCAACTAGCCGTTGGGAGCCTGGAATTCTCGGGTGCCAAGGAACTCC65690.11017453161987253RNA PCR Primer, Index 1 (100% over 27bp)
CTATGTTCGGGAAAGATAACCGCTTGGAATTCTCGGGTGCCAAGGAACTC65420.10972169064655292RNA PCR Primer, Index 1 (100% over 26bp)
AACACATGCAAGTCGAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT65010.10903404324262313RNA PCR Primer, Index 1 (100% over 30bp)
TGAGATTTGAGAGGGGCTGCTCCTTGGAATTCTCGGGTGCCAAGGAACTC64130.10755811710735921RNA PCR Primer, Index 1 (100% over 26bp)
GCGGCGCTGTCTCCACCCGAGACTCAGTGGAATTCTCGGGTGCCAAGGA64120.10754134521945849RNA PCR Primer, Index 1 (100% over 22bp)
CCTGAAACCGTATGCGTACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC63130.10588092831728656RNA PCR Primer, Index 1 (100% over 31bp)
AGCAGGGTCGGGCCTGGTTAGTACTTGGTGGAATTCTCGGGTGCCAAGG62800.10532745601656258Illumina Small RNA Adapter 2 (100% over 21bp)
TCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCA62330.10453917728522842No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)