FastQCFastQC Report
Tue 27 Jun 2023
SRR606201.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR606201.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12430537
Total Bases497.2 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAA3479432.79909870345907RNA PCR Primer, Index 4 (100% over 40bp)
AGTGATTAAGTTATTAAGAGCATGTGGTGGAATTCTCGGG2407891.9370764110995364No Hit
CCAATTTGGGGTAGGGACTCAGTTTGTTTTTTTTGGAATT2360731.899137583517108No Hit
TCCGGTCTTGAGGTCGAGAGAGGTGAGTGGAATTCTCGGG2251001.8108630383385689No Hit
CGATAGGGTCAAGTGACTAAGTGCATGTGGTGGAATTCTC2142081.7232401142444609No Hit
CAATTTTCAACTAATGGAGAACTAGCTGTTGGAATTCTCG2113261.7000552751663103No Hit
CCAATTAGGGGATGAGCTGTGGAATTCTCGGGTGCCAAGG2105091.6934827513887776Illumina Small RNA Adapter 2 (100% over 21bp)
AGTTGAGGTCGAGAGAGGTGAGTGGAATTCTCGGGTGCCA2104131.6927104597331555No Hit
ACTTTCGTGTGAAAGGTGGGTTGGTAATTGAGTTGGAATT2087881.6796378145208049No Hit
CATAGGGTCAAGTGACTAAGTGCATGTGGTGGAATTCTCG2029331.6325360682326113No Hit
GTGCTTTGTATTGGACTTTGAACAGATCTGTGTAGGATAG2005531.6133896709369835No Hit
CAAATGCAAGTTGTGGGGATGGGGCTTGGAATTCTCGGGT2003131.6114589417979288No Hit
CGAGTGAGGATTGACAGATTGAGAGCTCTTTCTGGAATTC1988961.6000595951727588No Hit
CAGATGAAGGATGCGAGCGTTATCCGGATTCACTGGAATT1913051.5389922414454018No Hit
CTTATAGGGTCAAGTGACTAAGTGCATGTGGTGGAATTCT1886151.517351985678495No Hit
AGCCTTGTGGCATGGAATTCTCGGGTGCCAAGGAACTCCA1865981.5011258162056875RNA PCR Primer, Index 1 (100% over 28bp)
CGAATGTGGGTGGTGGAATTCTCGGGTGCCAAGGAACTCC1864781.5001604516361602RNA PCR Primer, Index 1 (100% over 27bp)
AAGCGGATGAGCTGTGGATAGGGGTGAAAGGCTAAACAAA1829621.471875269749006No Hit
CGTATAGGGTCAAGTGACTAAGTGCATGTGGTGGAATTCT1795931.444772659459523No Hit
CATACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTG1744771.4036159499786693RNA PCR Primer, Index 4 (100% over 35bp)
CTCAGAGGGGAGTGGAATTCTCGGGTGCCAAGGAACTCCA1634531.3149311248580815RNA PCR Primer, Index 1 (100% over 28bp)
CTAAGCGTTACTTGGAATTCTCGGGTGCCAAGGAACTCCA1632341.3131693345186939RNA PCR Primer, Index 1 (100% over 28bp)
GATATTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTC1619721.3030169171291635RNA PCR Primer, Index 1 (100% over 31bp)
CAGCGTTATCCGGATTTACTGGAATTCTCGGGTGCCAAGG1494221.2020558725660846Illumina Small RNA Adapter 2 (100% over 21bp)
AGTAACACGGAATGGAGGACCGAACCCGTTAGCGTTGAAA1490531.1990873765147878No Hit
AGGTACTGGAGGACCGAACCCACTAACGTTGAAAAGTTAG1466191.1795065651628727No Hit
CTAAGGAAGGAGCCGTCGAAGGTGGAATTCTCGGGTGCCA1459511.1741327023925032No Hit
CCGAGTACGGCAGAGGGGGATGGAATTCTCGGGTGCCAAG1444501.162057600568664No Hit
CATTACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT1437901.1567480954362632RNA PCR Primer, Index 3 (100% over 34bp)
CAATGATAAATGTGGGTGGTGGAACTAGGCGTGCGACAAG1431021.1512133385709724No Hit
TAAGAGTGCGGTAGAGGGTAGTGGAATTCTCGGGTGCCAA1417531.1403610318685347No Hit
AATTTATTGCCGAATTGTTGATGGAACTGGAATTCTCGGG1398811.1253013445839066No Hit
CAAAGTGTTTTGTTTGGGTAGAGTATTATGTGTGAAGAGA1364121.0973942638198173No Hit
GTCGGTAGAGGGTAGTGGAATTCTCGGGTGCCAAGGAACT1334521.0735819377714735RNA PCR Primer, Index 1 (100% over 25bp)
CATTGTCGGGAGTTTTGGGTATTGCTGTATGTGGAATTCT1282211.031500087244823No Hit
CGAGTAGCGGATGAGCTGTGGAATTCTCGGGTGCCAAGGA1269781.0215005192454678RNA PCR Primer, Index 1 (100% over 22bp)
CCATTAGGGGATGAGCTGTGGAATTCTCGGGTGCCAAGGA1208860.9724921779324578RNA PCR Primer, Index 1 (100% over 22bp)
TCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGAC1203630.9682847973502674RNA PCR Primer, Index 4 (100% over 37bp)
ACACTGCACGGCTAGAGTGCGGCAGAGGGGAGTGGAATTC1199310.9648094848999687No Hit
CATATGGGTCAAGTGACTAAGTGCATGTGGTGGAATTCTC1175780.9458802946324846No Hit
CTAAAATTAGGGGATGAGCTGTGGAATTCTCGGGTGCCAA1174310.9446977230348134No Hit
CCTTAGGGGATGAGCTGTGGATAGGGGTGAAAGGCTAAAC1171430.9423808480679476No Hit
TAGATACTGTGAGGCTAGACGGTCGGAGAGGGCAGTGGAA1069930.8607270948954175No Hit
ACATAGCAAGGATTGACATACTGAGAGCTCTTTCTGGAAT1059380.8522399313883221No Hit
ATATTGACAGACTGAGAGCTCTTTCTGGAATTCTCGGGTG1053530.8475337791118759No Hit
CTTAACACGGATTGGAGGACCGAACCCGTTAGCGTTGAAA1028410.827325480789768No Hit
GCAGAGTGCGGTAGAGGGTAGTGGAATTCTCGGGTGCCAA1003400.8072056742198668No Hit
CCTTTGGGATGACTTGTGGAATTCTCGGGTGCCAAGGAAC985900.7931274409142581RNA PCR Primer, Index 1 (100% over 24bp)
CTTATAGGGTCAAGTGACTAAGTGCATGTGGAATTCTCGG975800.7850022891207354No Hit
AATCTTGAGTCCGGAAGAGGTGAGTGGAACTCCTAGTGTA937190.7539416840961899No Hit
CCCTTTGGGTTCCGGGGGGAGTTGGAATTCTCGGGTGCCA883510.7107577090193288No Hit
ACAATAGAAAAAGTGGTAAAAGGAAAGAGCAAATAAATGG877240.7057136791435479No Hit
AGGTATTACCATCGGGGGTAGAGCACTGTTATGGAATTCT876100.7047965828024968No Hit
AGAATAGCTCACTGGTCGAGTCGGTCTGCGTGGAATTCTC869640.6995997035365407No Hit
TTATTCGGTATTGAACTGGACGACGACACCACTGGAATTC868730.698867635404649No Hit
GCGTAGACGACTTAATGGAATTCTCGGGTGCCAAGGAACT867150.6975965720547712RNA PCR Primer, Index 1 (100% over 25bp)
CAAGACTTGAGTTCGGCAGGGGAGACTGGAATTCTCGGGT858040.6902678460311087No Hit
GCTGTTTCTTACCGGAGGTAGAGCTACTGGATGGAATTCT853560.6866638183048729No Hit
CGAAGTAGCGGATGAGCTGTGGAATTCTCGGGTGCCAAGG836910.6732693849026795Illumina Small RNA Adapter 2 (100% over 21bp)
GAGAAGTTATGAAGGACTCTGGAACTGGTTCCCTCTTGTT823500.6624814358382104No Hit
GCGTCAAGTGACTAAGAGCATATGGTGGATTCTCGGGTGC820950.6604300361279646No Hit
CCACTAGGGGATGAGCTGTGGATAGGGGTGAAAGGCTAAA814590.655313603909469No Hit
GAACGTACGACCATAGGTACTGGAATTCTCGGGTGCCAAG810040.6516532632500108No Hit
GGGAACCGACTAGTGTTGCAAAACTAGCTGATGAGCTGTG783180.6300451863020882No Hit
CAAAACACATTGGACTGACTGACAGGTTTCTTTATTGGAC773670.6223946720885831No Hit
CGCTGGAGGTCCGAACCGACTACTTGTAAAAGTAGCGGAT765200.6155808071686686No Hit
GCTCGGATCAGGGTTAGTCTGGACCTTAGGAGCCCCGAAA763810.614462593208966No Hit
CCGTTGAGTGAGAGCTGGAGTTAGGATGAGGGATGGGAGG758370.6100862738271082No Hit
CCCAATAAGGATTGACAGATTGAGAGCTCTTTCTGGAATT750810.6040044770390853No Hit
CCTTGGAAACTGGCGGGCTAGAGTGCGGTAGAGGGTAGTG745470.5997086047046881No Hit
CAACTGGAGGACCGAACGGGTGTCTGTTGAAAAGACTCTG743560.5981720660981903No Hit
CACAAAGTGGGTGGTTGTCCTCTGGAATTCTCGGGTGCCA736240.5922833422240729No Hit
CATAGTGAGGATTGACAGATTGAGAGCTCTTTCTGGAATT722690.5813827672931587No Hit
CCATCGCTTGGGTCGAGAGGGGCTGGAATTCTCGGGTGCC716940.5767570620641731No Hit
CGGTTCTACAGTCCGACGATCGAACTGGCGGGCTAGAGTG713020.5736035378037168No Hit
CCTTACAGGTGATGAAGGACGTGATAGCCTGGAATTCTCG705950.5679159315482508No Hit
TGCTCCGGGGGGTAGAGCACTGGAATTCTCGGGTGCCAAG689850.554963956907091No Hit
CGTTACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT680940.5477961249783497RNA PCR Primer, Index 3 (100% over 34bp)
CAACTACTGTTGCAAAAGTAGCGGATGAGCTGTGGAATTC675200.54317846445411No Hit
CAATGTGGATTATCCGCGGCAACGCAACTGAAGGTGGAAT675060.5430658385876652No Hit
CGGGCATTGGAAACTGGCGGGCTAGAGTGCGGTAGAGGGT609170.4900592790158623No Hit
AATTAACTTGAGTGCAGTAGAGGTAAGTGGAACTCCATGT607800.48895715446565186No Hit
TCATTGGAAACTGGCGGGCTAGAGTGCGGTAGAGGGTAGT580200.4667537693665205No Hit
GCTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAG555940.44723731565257396No Hit
CACGATGGATCTTCGTCGGGCCAGAATGGAATTCTCGGGT543250.43702858532982125No Hit
GCAGTGCGGTAGAGGGTAGTGGAATTCTCGGGTGCCAAGG539780.43423707278293766Illumina Small RNA Adapter 2 (100% over 21bp)
GCCGAGCGTTGTCCGGATTTATTGGAATTCTCGGGTGCCA535450.43075371562789283No Hit
GTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACC526570.4236100178133897RNA PCR Primer, Index 4 (100% over 38bp)
CGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACC481350.3872318629516971RNA PCR Primer, Index 4 (100% over 38bp)
TTCACACTGGGACTGAGACACGGTCCAGACTCCTACGGGA471750.37950894639547755No Hit
CGAAATGGATTTTTGGAAGTAGGAGTTGGAATTGGAATTC450420.3623495911721271No Hit
AGTAGGCCGACAAGTAGGGCGGGACACGTGGAATTCTCGG439820.3538222041413014No Hit
GCGAGTGCGGTAGAGGGTAGTGGAATTCTCGGGTGCCAAG438370.3526557219531224No Hit
AGCCGGTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC432020.34754733443937297RNA PCR Primer, Index 1 (100% over 31bp)
TCATGGTCGAGGTCGAGGCGGAAGTTGAATGGAATTCTCG418070.33632497131861644No Hit
CCGGTACTGGAGGTCCGAACCGACTAATGTTGCAAAATTA394910.3176934351267367No Hit
GCAGAGTGCGGCAGAGGGGAGTGGAATTCTCGGGTGCCAA391650.31507086137952045No Hit
CGCGTTAATCGGATCAGGGTTAGTCTGGACCTTAGGAGCA382550.30775018006060395No Hit
GGAGAGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC367860.29593250878863886RNA PCR Primer, Index 1 (100% over 33bp)
CCATGTGGGTGGTGGAACTAGGCGTGCACAAGTAGGGCGG358680.2885474698317539No Hit
GCGATTGCGGTAGAGGGTAGTGGAATTCTCGGGTGCCAAG327900.26378586862337483No Hit
CACTTGGATCCCTTAAGGTGAATGGAGCCAGGACTTGTTG317250.25521825806881876No Hit
CCATGTATACGGACTGACGCCTGCCCGGTGCTGGAATTCT311910.2509223857344216No Hit
GAAATTAAGGGCGTATGGGGGATGCCTAGGCTCTTGGAGG245780.19772275324871325No Hit
CCGAAAAGTTTTGGGATGACTTGTGGAATTCTCGGGTGCC209490.168528519725254No Hit
GCGAGGGTGCGAGCGTTGTCCGGATTTATTGGAATTCTCG180830.14547239592304018No Hit
CTTTAAAGGGTCGTTCGAGACCAGGACTTTGATAGGCTGG167270.13456377628738No Hit
CGGAAGAGGTAAGTGGAACTCCTAGTGTAGAGGTGGAATT166700.1341052281168545No Hit
CAAGACTTGAGTTCGGCAGGGGAGACTGGAATTCTCGGGG161080.1295841040495676No Hit
GCTGACTTAAGGGCATCTGGTGGATGCCTTGGCGGTAAGA139790.11245692764520149No Hit
TGTCTTAGATCGATGTGGTGCTCCGGAGTTCTCTTCGGGC134870.10849893291013896No Hit
AAGCGGATGAGCTGTGGATAGGGGGGAAAGGCTAAACAAA130890.10529714042120626No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)