FastQCFastQC Report
Tue 27 Jun 2023
SRR606202.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR606202.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8879905
Total Bases355.1 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCA736011682.88507591015895RNA PCR Primer, Index 3 (100% over 40bp)
GCGAGTGCGGTAGAGGGTAGTGGAATTCTCGGGTGCCAAG5411896.094535921273932No Hit
TCGAATTGCCTTTGGGACTGCGAGGCTTTGAGGACGGAAG2913303.2807783416601866No Hit
CCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGG2100512.365464495397192RNA PCR Primer, Index 3 (100% over 38bp)
CCGAAGGTTTTCAGCATCAAGAAAAGATGAGGCAGAAAAT257390.28985670454807794No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGAA185450.2088423243266679RNA PCR Primer, Index 3 (97% over 40bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGACA92840.10455066805331813RNA PCR Primer, Index 3 (97% over 40bp)
TGGAATTCTCGGGTGCCAAGGAAATCCAGTCACTTAGGCA91280.10279389250222835RNA PCR Primer, Index 3 (97% over 40bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)