FastQCFastQC Report
Sun 2 Jul 2023
SRR606203.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR606203.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences195019680
Total Bases7.8 Gbp
Sequences flagged as poor quality0
Sequence length40
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAA63252793.2434054860514587No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAA26351271.351210811134548No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA17767670.9110706160526979No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAA17518700.8983042121697666No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAA16376630.8397424300973112No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAA16373350.8395742419431721No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAA12988790.6660245776221149No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAA9904760.5078851529240537No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAAA9327380.478278910107944No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAA8104410.4155688287458989No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAA7970020.4086777293450589No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAA7837440.4018794410902531No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAA6562540.3365065515439262No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAAAAAA6502860.3334463475686146No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAAAAAAAA6387420.3275269449729381No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCATAAAAAAAAA6099180.31274689815920115No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAA6064080.31094707980240766No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTAAAAAAAAA5990120.3071546420340758No Hit
CCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAAA5910770.30308582190269207No Hit
CGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAA5788450.2968136343983335No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGTCAAAAAAAA5391380.27645312514101145No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAA5390390.2764023610335121No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAAAAAAAAAAA5236430.2685077731642263No Hit
TCGACTGCATAATTTGTGGTAGTGGGGTAAAAAAAAAAAA4911290.25183560961642437No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAA4857710.24908819458631049No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTAAAAAAAAAA4822420.24727863362302718No Hit
CGGGCCGCCGGTGAAATACCACTACTCTCATAAAAAAAAA4778880.245046038430583No Hit
GGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAA4484150.22993320468990616No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCAAAAAAAAAAAA4424960.22689812638396287No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGACAAAAAAAA4023890.20633250962159305No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGTAAAAAAAAA3947950.20243854363826255No Hit
GGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAAA3916530.20082742418611294No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAAA3908840.20043310500765874No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTAAAAAAA3834510.19662169479510988No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTAAAAAAAA3772210.19342714540399206No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCACAAAAAAAAA3734360.19148631563747823No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAA3502620.17960341233253999No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAA3441730.1764811633369514No Hit
GGGGCCGCCGGTGAAATACCACTACTCTCATAAAAAAAAA3431350.17594890936135266No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGTAAAAAAAAAAA3355590.1720641732157493No Hit
TCGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAA3277270.16804816826691543No Hit
TGCTGAGAAGACGGTCGAACTTGACTATCTAAAAAAAAAA3248480.16657190699933463No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAA2902740.14884343980053705No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGTTAAAAAAAA2899490.14867678995268582No Hit
TCGGGCCGCCGGTGAAATACCACTACTCTCATAAAAAAAA2831950.14521354972995545No Hit
GCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAA2812000.14419057604853008No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCGTAAAAAAAAA2767270.1418969613733342No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAAA2763210.1416887772557108No Hit
GGGCCGCCGGTGAAATACCACTACTCTCATAAAAAAAAAA2734360.1402094393755543No Hit
GGGGGGTGGGGGCCGGGCCGCAAAAAAAAAAAAAAAAAAA2684530.13765431263142264No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAA2543410.13041811985333993No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCACAAAAAAAAA2516290.12902749096911656No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAA2382010.1221420320246654No Hit
CCGGGGTCCGGTGCGGAGAGCCGTTCAAAAAAAAAAAAAA2359760.12100112152783761No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAA2341800.12008018883017345No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAA2241760.11495045012893058No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAA2240300.11487558588958817No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAAAAAAAAA2166720.1111026333342358No Hit
TCGCTGAGAAGACGGTCGAACTTGACTATCTAAAAAAAAA2136420.10954894398349951No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGTAAAAAAAA2133510.10939972827357732No Hit
CGGGCCGCCGGTGAAATACCACTACTCTCGTAAAAAAAAA2132280.10933665771577514No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAA2087090.1070194556774988No Hit
GCGCAGCGGAAGCGTGCTGGGCAAAAAAAAAAAAAAAAAA2057720.10551345382168609No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)