FastQCFastQC Report
Tue 27 Jun 2023
SRR606204.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR606204.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences187295054
Total Bases7.4 Gbp
Sequences flagged as poor quality0
Sequence length40
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAA55542572.9655118388764286No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA25226041.3468609801089568No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAA24251881.2948489285787548No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAA19621761.0476389835686746No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAA15233670.813351429984905No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAA13382670.7145234064750049No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAA12032700.6424462228457992No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAAA10511240.5612128978056196No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTAAAAAAAAA10405750.5555806081243341No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAA9644140.5149169609145151No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAA8325230.444498123265978No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAA7458640.39822941613823926No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTAAAAAAAA6789880.3625231876117775No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCATAAAAAAAAA6709190.3582150119137689No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAAAAAA6665400.3558769896828135No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAAAAAAAA6598300.35229440709096355No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAA6374850.34036403331825305No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAA6150370.3283786661018822No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCACAAAAAAAAA6135760.3275986134689921No Hit
GGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAA6011720.3209759078848927No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAA5943780.3173484762710285No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAAA5603490.29917981710291186No Hit
CGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAA5469090.2920039735806371No Hit
GGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAAA5294310.2826721735001075No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGTAAAAAAAAA4849290.2589118023372897No Hit
GGGGCCGCCGGTGAAATACCACTACTCTCATAAAAAAAAA4819480.2573201959727137No Hit
CGGGCCGCCGGTGAAATACCACTACTCTCATAAAAAAAAA4814750.25706765326541936No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAA4595660.2453700672736398No Hit
GGGCCGCCGGTGAAATACCACTACTCTCATAAAAAAAAAA4499040.24021136190814735No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAA4495890.240043178075594No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGTCAAAAAAAA4260720.22748705366239944No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAA4064330.21700145909886118No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAA3881650.20724786464462647No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGACAAAAAAAA3442790.18381638630991293No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTAAAAAAA2963750.15823962975551933No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCTAAAAAAAAAA2936420.15678043478927103No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGTAAAAAAAA2921620.15599023773473483No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAAAAAAAAA2758590.1472857900454755No Hit
TGGCCGCCGGTGAAATACCACTACTCTCATAAAAAAAAAA2742610.14643259079334792No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAA2727870.14564559724038414No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCGTAAAAAAAAA2643490.14114040619567028No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAA2631010.14047407786860192No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGTTAAAAAAAA2626630.1402402222538135No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGACAAAAAAAAA2597310.13867477781874582No Hit
CCGGGGTCCGGTGCGGAGAGCCGTTCAAAAAAAAAAAAAA2591190.13834802065835652No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAA2527030.1349224096435563No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCACAAAAAAAAA2332060.12451263128389926No Hit
TGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAAA2208430.1179118162938782No Hit
TCGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAA2201900.11756316853941055No Hit
GGATGGTTTAGTGAGGCCCTCGGATCGGCAAAAAAAAAAA2116400.11299817879867774No Hit
CCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAAA2047580.10932376249508437No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCATAAAAAAA1999380.10675028289855429No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCAAAAAAAAAA1961660.10473634824334442No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGAAAAAAAAAA1931310.10311591036461645No Hit
GGGGCCGCCGGTGAAATACCACTACTCTCGTAAAAAAAAA1909770.10196585330010904No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAA1902070.1015547372649787No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTAAAAAAA1892080.10102135425316676No Hit
TGGGCCGCCGGTGAAATACCACTACTCTAAAAAAAAAAAA1890190.10092044395363477No Hit
CGGGCCGCCGGTGAAATACCACTACTCTCGTAAAAAAAAA1878830.10031391432258537No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)