FastQCFastQC Report
Tue 27 Jun 2023
SRR606205.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR606205.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences189624054
Total Bases7.5 Gbp
Sequences flagged as poor quality0
Sequence length40
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAA46757352.4657921299372707No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAA30101891.587451030869744No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA24570371.2957412038031841No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAA18021760.9503941941880433No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAA16771940.8844837796791328No Hit
CCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAAA16687470.8800291760453555No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAA15449760.8147573935952239No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAA13612670.7178767520707051No Hit
GGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAAA12165950.6415826338150117No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAAA11606640.6120869032786316No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTAAAAAAAAA10291130.542712265818344No Hit
GCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAA10098580.5325579633478356No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAA9879870.5210240890641437No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAA9802900.5169650048722194No Hit
GGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAA8601320.45359857141330817No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAA8488330.4476399391819774No Hit
CGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAA7122880.37563166959820404No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAA7120820.3755230335915084No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAA6892480.36348131234447717No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCATAAAAAAAAA6564960.34620924199838066No Hit
CCGGGGTCCGGTGCGGAGAGCCGTTCAAAAAAAAAAAAAA6361850.3354980481537432No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAA6128020.3231668066752755No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCACAAAAAAAAA6035740.3183003354627151No Hit
GGGCCGCCGGTGAAATACCACTACTCTCATAAAAAAAAAA6019370.3174370483609637No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAAA5818000.3068176150268362No Hit
TGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAAA5781370.30488589807282573No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAA5603040.2954815004640709No Hit
GGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAA5426380.2861651718510353No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAAAAAAAA5356160.2824620551567788No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGTAAAAAAAAA5156970.27195758614041654No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAA5006490.2640218840590762No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTAAAAAAAA4362490.23005994798529095No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCTAAAAAAAAAA4148660.21878342501843148No Hit
TGGCCGCCGGTGAAATACCACTACTCTCATAAAAAAAAAA4048520.21350244943080904No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGTCAAAAAAAA4040230.2130652686077474No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAA3902640.20580933260713857No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAA3837290.20236303987045864No Hit
GGATGGTTTAGTGAGGCCCTCGGATCGGCAAAAAAAAAAA3717650.19605371373401814No Hit
TGGGCCGCCGGTGAAATACCACTACTCTAAAAAAAAAAAA3696430.19493465739320182No Hit
GGGGCCGCCGGTGAAATACCACTACTCTCATAAAAAAAAA3674630.19378501421554883No Hit
CGGGCCGCCGGTGAAATACCACTACTCTCATAAAAAAAAA3443370.1815893040658228No Hit
TGGGCCGCCGGTGAAATACCACTACTCCCAAAAAAAAAAA3385110.178516909041508No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGACAAAAAAAA3144080.16580596889886132No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGAAAAAAAAAA3131200.16512673017738563No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAA3073850.162102324845349No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAA3052760.1609901241748581No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAAAAAAAAAAA2977860.15704020334888524No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAA2943230.15521395824603562No Hit
GGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAAAA2901170.15299588521612348No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGACAAAAAAAAA2877950.15177135702414632No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCAAAAAAAAAA2859120.15077833954546715No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGTAAAAAAAA2805860.14796962414905443No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAA2774060.14629262171559734No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTAAAAAAAAAA2737090.1443429745468895No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAAA2722040.1435492988669043No Hit
CCGGGGTCCGGTGCGGAGAGCCGTTCGAAAAAAAAAAAAA2668810.14074216554825897No Hit
GGCCGCCGGTGAAATACCACTACTCTCATAAAAAAAAAAA2647480.1396173082556288No Hit
GGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAA2614330.13786911232263813No Hit
CCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAAAAAA2501430.13191522632460964No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAA2485810.1310914911670436No Hit
TCGACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAAA2461820.12982635631236952No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAAAAAAAAA2391290.1261068914811831No Hit
GGGCCGCCGGTGAAATACCACTACTCCCAAAAAAAAAAAA2266080.1195038262392597No Hit
TGCTGAGAAGACGGTCGAACTTGACTATCTAAAAAAAAAA2247210.11850869932355733No Hit
TCGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAA2221010.11712701807335055No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCCAAAAAAAAAA2215830.11685384597884402No Hit
GGGGCCGCCGGTGAAATACCACTACTCTAAAAAAAAAAAA2160300.11392542003136373No Hit
GGATGGTTTAGTGAGGCCCTCGGATCGGAAAAAAAAAAAA2135500.11261756907696953No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCGTTAAAAAAAAAA2113090.1114357569847125No Hit
CCCAAGTCCTTCTGATCGAGGCCTAAAAAAAAAAAAAAAA2092390.11034412332519797No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCGTAAAAAAAAA2037080.10742729928134538No Hit
TCGACTGCATAATTTGTGGTAGTGGGGTAAAAAAAAAAAA2015060.10626605420006473No Hit
TGGGCCGCCGGTGAAATACCACTACTCTTAAAAAAAAAAA1981060.10447303273033073No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAA1964270.1035875965398356No Hit
CCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAAAA1948440.1027527868378977No Hit
CCGCCGGTGAAATACCACTACTCTCATAAAAAAAAAAAAA1947500.10270321506785211No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTAAAAAAA1947130.10268370277538735No Hit
GGGGCCGCCGGTGAAATACCACTACTCCCAAAAAAAAAAA1944380.10253867898004122No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCAAAAAAAAAAAAAA1935120.10205034430916661No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCAAAAAAAAAAAA1911920.10082687083570105No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGTAAAAAAAAAA1901350.1002694521023161No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)