FastQCFastQC Report
Tue 4 Jul 2023
SRR6075747_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6075747_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41356422
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length30-70
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG24671715.965629715259216Illumina Small RNA Adapter 2 (100% over 21bp)
CCCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAGAT24142625.837695533719043RNA PCR Primer, Index 1 (95% over 22bp)
CCTCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAGA23332995.641926663771832RNA PCR Primer, Index 1 (95% over 22bp)
CCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTG7232051.7487126908609258No Hit
CCTCCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAG7206891.742628992421056RNA PCR Primer, Index 1 (95% over 22bp)
CCTCACGTTCTAGCATTCAAGGTCCCATGGAATTCTCGGGTGCCAAGGAG6409571.549836685581746RNA PCR Primer, Index 1 (95% over 22bp)
CCCACGTTCTAGCATTCAAGGTCCCATGGAATTCTCGGGTGCCAAGGAGA6376951.5419491560464298RNA PCR Primer, Index 1 (95% over 22bp)
CCAAGAGGTGCACAATCGACCGATCCTGTGGAATTCTCGGGTGCCAAGGA5777531.397009151323584RNA PCR Primer, Index 1 (100% over 22bp)
CCCACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGCCAAGGAGATC5697161.3775756519749218RNA PCR Primer, Index 1 (95% over 22bp)
CCAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGGA5259891.2718435845344649RNA PCR Primer, Index 1 (100% over 22bp)
CCTCACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGCCAAGGAGAT5235151.2658614422688694RNA PCR Primer, Index 1 (95% over 22bp)
CCCCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAGA3602830.8711657889553405RNA PCR Primer, Index 1 (95% over 22bp)
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT3554090.8593804367312047No Hit
CCTTTTCCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAG2801320.677360338377435No Hit
CCCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAG2735150.6613604049209093No Hit
CCTTTCCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGG2483750.6005717806051983Illumina Small RNA Adapter 2 (100% over 21bp)
CCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAGATC2268450.548512151268792RNA PCR Primer, Index 1 (95% over 22bp)
CCTGCCTTGTTGTAGACGGCCTTGGTGGAATTCTCGGGTGCCAAGGAGAT2153640.5207510456296244RNA PCR Primer, Index 1 (95% over 22bp)
CCTTGCTCGAATATATTAGCATGGAATAATAGAATATGGAATTCTCGGGT2118140.5121671308992833No Hit
CCGCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAGA1977000.4780394203347669RNA PCR Primer, Index 1 (95% over 22bp)
CCTCCACGTTCTAGCATTCAAGGTCCCATGGAATTCTCGGGTGCCAAGGA1739350.4205755517244698RNA PCR Primer, Index 1 (100% over 22bp)
CCTCCACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGCCAAGGAGA1688100.4081832804588366RNA PCR Primer, Index 1 (95% over 22bp)
CCAGAGGTGCACAATCGACCGATCCTGTGGAATTCTCGGGTGCCAAGGAG1427140.34508304417630714RNA PCR Primer, Index 1 (95% over 22bp)
CCTGCTCTGCTAGGCGGACTACTTGCGTGCCTGGAATTCTCGGGTGCCAA1400880.33873336528000414No Hit
CCTTGCCTTGTTGTAGACGGCCTTGGTGGAATTCTCGGGTGCCAAGGAGA1358490.3284834456907321RNA PCR Primer, Index 1 (95% over 22bp)
CCTGCCTTGTTGTAGACGGCCTTGGTTGGAATTCTCGGGTGCCAAGGAGA1252570.3028719457403738RNA PCR Primer, Index 1 (95% over 22bp)
CCCCCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAG1250690.3024173609602881RNA PCR Primer, Index 1 (95% over 22bp)
CCAAATATAGATGGATACGAATAAGGCGTCCTGGAATTCTCGGGTGCCAA1244180.30084324025903403No Hit
CCTTTTCCACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGCCAAGG1232200.29794647128806256Illumina Small RNA Adapter 2 (100% over 21bp)
CCTGCTCTGCTAGGCGGACTACTTGCGTGCCTTGGAATTCTCGGGTGCCA1220130.29502794027974666No Hit
CCTTCCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGA1130580.2733747131219427RNA PCR Primer, Index 1 (100% over 22bp)
CCTATAGATGGATACGAATAAGGCGTCCTGGAATTCTCGGGTGCCAAGGA1075220.25998864215090944RNA PCR Primer, Index 1 (100% over 22bp)
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATATGGAATTCTCGGG1042360.2520430805160079No Hit
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT1011860.24466816786036277Illumina Paired End PCR Primer 2 (100% over 27bp)
CCAAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAG977440.23634539757815606No Hit
CCCCACGTTCTAGCATTCAAGGTCCCATGGAATTCTCGGGTGCCAAGGAG956840.23136430903040886RNA PCR Primer, Index 1 (95% over 22bp)
CCTGTTGTAGACGGCCTTGGTTGGAATTCTCGGGTGCCAAGGAGATCGGA949340.22955080591836502RNA PCR Primer, Index 1 (95% over 22bp)
CCATATAGATGGATACGAATAAGGCGTCCTGGAATTCTCGGGTGCCAAGG911060.220294686034493Illumina Small RNA Adapter 2 (100% over 21bp)
CCTTTCCACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGCCAAGGA902670.21826598055315324RNA PCR Primer, Index 1 (100% over 22bp)
CCTTGCCTTGTTGTAGACGGCCTTGGTTGGAATTCTCGGGTGCCAAGGAG885570.21413119345769321RNA PCR Primer, Index 1 (95% over 22bp)
CCAAATATAGATGGATACGAATAAGGCGTCTGGAATTCTCGGGTGCCAAG828670.20037274984765363No Hit
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG816730.1974856528932798No Hit
CCCCACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGCCAAGGAGAT810170.19589944217127875RNA PCR Primer, Index 1 (95% over 22bp)
CCTAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG684710.1655631621130087Illumina Small RNA Adapter 2 (100% over 21bp)
CCCAAGAGGTGCACAATCGACCGATCCTGTGGAATTCTCGGGTGCCAAGG674020.16297831567730883Illumina Small RNA Adapter 2 (100% over 21bp)
CCTATAGATGGATACGAATAAGGCGTCTGGAATTCTCGGGTGCCAAGGAG672140.1625237308972232RNA PCR Primer, Index 1 (95% over 22bp)
CCTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTGC667710.16145255505904257No Hit
CCTTTTCCACGTTCTAGCATTCAAGGTCCCATGGAATTCTCGGGTGCCAA625290.1511953814573224No Hit
CCAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAA613740.14840258666477482No Hit
CCACACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAGA612780.1481704582664332RNA PCR Primer, Index 1 (95% over 22bp)
CCTAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAG588260.1422415120921244No Hit
CCTTTCCACGTTCTAGCATTCAAGGTCCCATGGAATTCTCGGGTGCCAAG583450.14107845209626693No Hit
CCTGTTGTAGACGGCCTTGGTGGAATTCTCGGGTGCCAAGGAGATCGGAA576140.13931089106306152RNA PCR Primer, Index 1 (95% over 22bp)
CCATATAGATGGATACGAATAAGGCGTCTGGAATTCTCGGGTGCCAAGGA572220.13836303343649992RNA PCR Primer, Index 1 (100% over 22bp)
CCGCACGTTCTAGCATTCAAGGTCCCATGGAATTCTCGGGTGCCAAGGAG539890.1305456260215161RNA PCR Primer, Index 1 (95% over 22bp)
CCGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAAG522020.12622465260655286No Hit
CCACGTTCTAGCATTCAAGGTCCCATGGAATTCTCGGGTGCCAAGGAGAT518810.12544847327459807RNA PCR Primer, Index 1 (95% over 22bp)
CCTTCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAG501050.12115409790527816RNA PCR Primer, Index 1 (95% over 22bp)
CCACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGCCAAGGAGATCG500870.12111057383058911RNA PCR Primer, Index 1 (95% over 22bp)
CCGCCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAG493010.11921002256916713RNA PCR Primer, Index 1 (95% over 22bp)
CCCCAACGGGGCCTTTCCTTCTGGCTGGAATTCTCGGGTGCCAAGGAGAT488290.11806872461065418RNA PCR Primer, Index 1 (95% over 22bp)
CCTGCGTGCCTTGTTGTAGACGGCCTTGGTGGAATTCTCGGGTGCCAAGG488000.11799860249032182Illumina Small RNA Adapter 2 (100% over 21bp)
CCAAGAGGTACACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG482950.11677751039487895Illumina Small RNA Adapter 2 (100% over 21bp)
CCTCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAA479340.11590461089694848No Hit
CCAAGAGGTGTACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG454550.1099103786106061Illumina Small RNA Adapter 2 (100% over 21bp)
CCGCACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGCCAAGGAGAT452470.10940743374753262RNA PCR Primer, Index 1 (95% over 22bp)
CCCAATATAGATGGATACGAATAAGGCGTCCTGGAATTCTCGGGTGCCAA439080.10616972619149695No Hit
CCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGTCAAGG436370.10551444706701175Illumina Small RNA Adapter 2 (95% over 21bp)
CCGCTCTGCTAGGCGGACTACTTGCGTGCCTGGAATTCTCGGGTGCCAAG435430.10528715467696892No Hit
CCGCTCTGCTAGGCGGACTACTTGCGTGCCTTGGAATTCTCGGGTGCCAA416770.10077515893420373No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)