FastQCFastQC Report
Thu 6 Jul 2023
SRR6075748_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6075748_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39290001
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length30-70
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAGAT403220710.262679810061599RNA PCR Primer, Index 1 (95% over 22bp)
CCTCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAGA35404659.011109467775274RNA PCR Primer, Index 1 (95% over 22bp)
CCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTG20805685.295413456466952No Hit
CCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG16044664.083649679723857Illumina Small RNA Adapter 2 (100% over 21bp)
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT8746372.2261058227002843No Hit
CCCACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGCCAAGGAGATC8509842.1659047552582145RNA PCR Primer, Index 1 (95% over 22bp)
CCTCACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGCCAAGGAGAT6922811.7619775575979242RNA PCR Primer, Index 1 (95% over 22bp)
CCAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGGA6324131.6096029114379506RNA PCR Primer, Index 1 (100% over 22bp)
CCAAGAGGTGCACAATCGACCGATCCTGTGGAATTCTCGGGTGCCAAGGA5743131.4617281379046032RNA PCR Primer, Index 1 (100% over 22bp)
CCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAGATC4835471.2307126182053292RNA PCR Primer, Index 1 (95% over 22bp)
CCCCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAGA4182681.0645660202452019RNA PCR Primer, Index 1 (95% over 22bp)
CCCACGTTCTAGCATTCAAGGTCCCATGGAATTCTCGGGTGCCAAGGAGA3538820.9006922651898125RNA PCR Primer, Index 1 (95% over 22bp)
CCTCACGTTCTAGCATTCAAGGTCCCATGGAATTCTCGGGTGCCAAGGAG3340480.8502112280424732RNA PCR Primer, Index 1 (95% over 22bp)
CCTCCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAG3164050.8053066733187407RNA PCR Primer, Index 1 (95% over 22bp)
CCGCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAGA2933720.7466836155081799RNA PCR Primer, Index 1 (95% over 22bp)
CCAGAGGTGCACAATCGACCGATCCTGTGGAATTCTCGGGTGCCAAGGAG2603530.6626444219230231RNA PCR Primer, Index 1 (95% over 22bp)
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT2565000.6528378556162419Illumina Paired End PCR Primer 2 (100% over 27bp)
CCTTGCTCGAATATATTAGCATGGAATAATAGAATATGGAATTCTCGGGT2310180.5879816597612202No Hit
CCTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTGC2036410.5183023538227958No Hit
CCTATAGATGGATACGAATAAGGCGTCCTGGAATTCTCGGGTGCCAAGGA1686010.42911935787428457RNA PCR Primer, Index 1 (100% over 22bp)
CCTATAGATGGATACGAATAAGGCGTCTGGAATTCTCGGGTGCCAAGGAG1560720.3972308374336768RNA PCR Primer, Index 1 (95% over 22bp)
CCATATAGATGGATACGAATAAGGCGTCCTGGAATTCTCGGGTGCCAAGG1230770.31325272809232046Illumina Small RNA Adapter 2 (100% over 21bp)
CCAAATATAGATGGATACGAATAAGGCGTCTGGAATTCTCGGGTGCCAAG1198110.3049401805818228No Hit
CCAAATATAGATGGATACGAATAAGGCGTCCTGGAATTCTCGGGTGCCAA1196890.3046296690091711No Hit
CCATATAGATGGATACGAATAAGGCGTCTGGAATTCTCGGGTGCCAAGGA1178530.2999567243584443RNA PCR Primer, Index 1 (100% over 22bp)
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATATGGAATTCTCGGG962190.2448943689260787No Hit
CCACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGCCAAGGAGATCG937440.23859505628416755RNA PCR Primer, Index 1 (95% over 22bp)
CCACACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAGA909400.23145838046682668RNA PCR Primer, Index 1 (95% over 22bp)
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG864260.21996945227871081No Hit
CCCCACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGCCAAGGAGAT827880.21071009898930773RNA PCR Primer, Index 1 (95% over 22bp)
CCCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAG708880.18042249477163413No Hit
CCTTCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAG694280.17670653660711286RNA PCR Primer, Index 1 (95% over 22bp)
CCTGCCTTGTTGTAGACGGCCTTGGTGGAATTCTCGGGTGCCAAGGAGAT660910.16821328154203916RNA PCR Primer, Index 1 (95% over 22bp)
CCTCCACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGCCAAGGAGA644430.16401883013441512RNA PCR Primer, Index 1 (95% over 22bp)
CCAAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAG588020.14966148766450782No Hit
CCGCACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGCCAAGGAGAT578820.1473199249854944RNA PCR Primer, Index 1 (95% over 22bp)
CCCCCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAG572970.14583099654286086RNA PCR Primer, Index 1 (95% over 22bp)
CCTAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG555750.14144820205018574Illumina Small RNA Adapter 2 (100% over 21bp)
CCTTGCTCGAATATATTAGCATGGAATTCTCGGGTGCCAAGGAGATCGGA531340.13523542542032513RNA PCR Primer, Index 1 (95% over 22bp)
CCTGCCTTGTTGTAGACGGCCTTGGTTGGAATTCTCGGGTGCCAAGGAGA514600.1309747994152507RNA PCR Primer, Index 1 (95% over 22bp)
CCTTTCCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGG496430.1263502131241992Illumina Small RNA Adapter 2 (100% over 21bp)
CTCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAGAT493920.12571137374112054RNA PCR Primer, Index 1 (95% over 22bp)
CCTGCTCTGCTAGGCGGACTACTTGCGTGCCTGGAATTCTCGGGTGCCAA465490.11847543602760406No Hit
CCAATATAGATGGATACGAATAAGGCGTCCTGGAATTCTCGGGTGCCAAG461470.11745227494394822No Hit
CCTCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGTCAAGGAGA461380.11742936835252309RNA PCR Primer, Index 1 (95% over 22bp)
CCAATATAGATGGATACGAATAAGGCGTCTGGAATTCTCGGGTGCCAAGG447030.11377703960862715Illumina Small RNA Adapter 2 (100% over 21bp)
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG422910.10763807310669195No Hit
CCGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAAG415010.10562738341493043No Hit
CCCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGTCAAGGAGAT410940.10459149644714949RNA PCR Primer, Index 1 (95% over 22bp)
CCTTTTCCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAG409010.10410027731992168No Hit
CCTTGCCTTGTTGTAGACGGCCTTGGTGGAATTCTCGGGTGCCAAGGAGA402970.10256299051761286RNA PCR Primer, Index 1 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)