FastQCFastQC Report
Fri 7 Jul 2023
SRR6075749_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6075749_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40868299
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length31-70
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAGA32661487.991886327346289RNA PCR Primer, Index 1 (95% over 22bp)
CCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG26208116.412821341059486Illumina Small RNA Adapter 2 (100% over 21bp)
CCCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAGAT22845895.590125001287673RNA PCR Primer, Index 1 (95% over 22bp)
CCTCCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAG8797902.1527443557168846RNA PCR Primer, Index 1 (95% over 22bp)
CCTCACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGCCAAGGAGAT6668891.6318002371471345RNA PCR Primer, Index 1 (95% over 22bp)
CCAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGGA5784581.4154198098628965RNA PCR Primer, Index 1 (100% over 22bp)
CCCACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGCCAAGGAGATC4933981.2072878296207044RNA PCR Primer, Index 1 (95% over 22bp)
CCAAGAGGTGCACAATCGACCGATCCTGTGGAATTCTCGGGTGCCAAGGA4775251.1684484348125181RNA PCR Primer, Index 1 (100% over 22bp)
CCCCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAGA4681781.145577407075347RNA PCR Primer, Index 1 (95% over 22bp)
CCTCACGTTCTAGCATTCAAGGTCCCATGGAATTCTCGGGTGCCAAGGAG4090281.0008441995591744RNA PCR Primer, Index 1 (95% over 22bp)
CCTTTTCCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAG3994100.9773100661713373No Hit
CCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTG3831920.9376264962728201No Hit
CCTTTCCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGG3293550.8058935851477449Illumina Small RNA Adapter 2 (100% over 21bp)
CCCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAG2954220.7228634595239699No Hit
CCGCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAGA2873680.7031562532123004RNA PCR Primer, Index 1 (95% over 22bp)
CCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAGATC2714940.6643144115197943RNA PCR Primer, Index 1 (95% over 22bp)
CCCACGTTCTAGCATTCAAGGTCCCATGGAATTCTCGGGTGCCAAGGAGA2707760.662557548578178RNA PCR Primer, Index 1 (95% over 22bp)
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT2629170.6433274847088694No Hit
CCTCCACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGCCAAGGAGA1824900.44653191952031085RNA PCR Primer, Index 1 (95% over 22bp)
CCAAATATAGATGGATACGAATAAGGCGTCCTGGAATTCTCGGGTGCCAA1824200.4463606376179248No Hit
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT1780790.43573871278567283Illumina Paired End PCR Primer 2 (100% over 27bp)
CCCCCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAG1679370.4109224120142608RNA PCR Primer, Index 1 (95% over 22bp)
CCTTTTCCACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGCCAAGG1534570.37549152706355604Illumina Small RNA Adapter 2 (100% over 21bp)
CCTATAGATGGATACGAATAAGGCGTCCTGGAATTCTCGGGTGCCAAGGA1418280.34703670930860125RNA PCR Primer, Index 1 (100% over 22bp)
CCTGCCTTGTTGTAGACGGCCTTGGTGGAATTCTCGGGTGCCAAGGAGAT1414860.34619987487122966RNA PCR Primer, Index 1 (95% over 22bp)
CCTGCTCTGCTAGGCGGACTACTTGCGTGCCTGGAATTCTCGGGTGCCAA1342560.3285089012390753No Hit
CCTTGCCTTGTTGTAGACGGCCTTGGTGGAATTCTCGGGTGCCAAGGAGA1314130.32155240911788374RNA PCR Primer, Index 1 (95% over 22bp)
CCTTCCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGA1212270.2966284454364005RNA PCR Primer, Index 1 (100% over 22bp)
CCATATAGATGGATACGAATAAGGCGTCCTGGAATTCTCGGGTGCCAAGG1189120.29096390823606333Illumina Small RNA Adapter 2 (100% over 21bp)
CCAAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAG1171740.2867112232882509No Hit
CCTGCTCTGCTAGGCGGACTACTTGCGTGCCTTGGAATTCTCGGGTGCCA1090100.26673485970140326No Hit
CCAGAGGTGCACAATCGACCGATCCTGTGGAATTCTCGGGTGCCAAGGAG1085360.26557503653381803RNA PCR Primer, Index 1 (95% over 22bp)
CCTTTCCACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGCCAAGGA1036030.25350455618424445RNA PCR Primer, Index 1 (100% over 22bp)
CCTTGCCTTGTTGTAGACGGCCTTGGTTGGAATTCTCGGGTGCCAAGGAG1025460.25091819945821575RNA PCR Primer, Index 1 (95% over 22bp)
CCAAATATAGATGGATACGAATAAGGCGTCTGGAATTCTCGGGTGCCAAG1020510.24970699171991476No Hit
CCTCCACGTTCTAGCATTCAAGGTCCCATGGAATTCTCGGGTGCCAAGGA995940.243694996946166RNA PCR Primer, Index 1 (100% over 22bp)
CCTGCCTTGTTGTAGACGGCCTTGGTTGGAATTCTCGGGTGCCAAGGAGA989260.24206047822053958RNA PCR Primer, Index 1 (95% over 22bp)
CCTTCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAG964390.23597507691719685RNA PCR Primer, Index 1 (95% over 22bp)
CCCCACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGCCAAGGAGAT962970.23562761934378526RNA PCR Primer, Index 1 (95% over 22bp)
CCACACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAGA881930.21579806881612565RNA PCR Primer, Index 1 (95% over 22bp)
CCTGTTGTAGACGGCCTTGGTTGGAATTCTCGGGTGCCAAGGAGATCGGA821830.20109229405412737RNA PCR Primer, Index 1 (95% over 22bp)
CCTAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG802650.1963991699287509Illumina Small RNA Adapter 2 (100% over 21bp)
CCTAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAG745170.1823344788585402No Hit
CCTATAGATGGATACGAATAAGGCGTCTGGAATTCTCGGGTGCCAAGGAG725700.17757039508788952RNA PCR Primer, Index 1 (95% over 22bp)
CCTCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAA670460.16405380610531403No Hit
CCATATAGATGGATACGAATAAGGCGTCTGGAATTCTCGGGTGCCAAGGA631000.15439840057938306RNA PCR Primer, Index 1 (100% over 22bp)
CCGCCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAG622020.1522010984602026RNA PCR Primer, Index 1 (95% over 22bp)
CCTGTTGTAGACGGCCTTGGTGGAATTCTCGGGTGCCAAGGAGATCGGAA611200.14955356962617897RNA PCR Primer, Index 1 (95% over 22bp)
CCGCACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGCCAAGGAGAT601930.14728530786172433RNA PCR Primer, Index 1 (95% over 22bp)
CCTCAATATAGATGGATACGAATAAGGCGTCCTGGAATTCTCGGGTGCCA578090.14145193564332098No Hit
CCCCAACGGGGCCTTTCCTTCTGGCTGGAATTCTCGGGTGCCAAGGAGAT575420.1407986175299344RNA PCR Primer, Index 1 (95% over 22bp)
CCCCACGTTCTAGCATTCAAGGTCCCATGGAATTCTCGGGTGCCAAGGAG572360.14004987092807558RNA PCR Primer, Index 1 (95% over 22bp)
CCCAAGAGGTGCACAATCGACCGATCCTGTGGAATTCTCGGGTGCCAAGG562880.13773022459290513Illumina Small RNA Adapter 2 (100% over 21bp)
CCACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGCCAAGGAGATCG551750.13500684234496765RNA PCR Primer, Index 1 (95% over 22bp)
CCTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTGC551670.13498726727040927No Hit
CCAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAA531090.12995157934026078No Hit
CCAAGAGGTGTACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG517140.1265381757141397Illumina Small RNA Adapter 2 (100% over 21bp)
CCCAATATAGATGGATACGAATAAGGCGTCCTGGAATTCTCGGGTGCCAA512790.12547378103502668No Hit
CCAAGAGGTACACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG487620.11931497320208995Illumina Small RNA Adapter 2 (100% over 21bp)
CCGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAAG477650.1168754295352493No Hit
CCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGTCAAGG475610.1163762651340101Illumina Small RNA Adapter 2 (95% over 21bp)
CCTCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGTCAAGGAGA459340.11239518434569543RNA PCR Primer, Index 1 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)