FastQCFastQC Report
Tue 4 Jul 2023
SRR6075750_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6075750_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38776786
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length30-70
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG23864376.154292931858767Illumina Small RNA Adapter 2 (100% over 21bp)
CCTCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAGA15005803.8697895178831994RNA PCR Primer, Index 1 (95% over 22bp)
CCCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAGAT13801103.5591139502897433RNA PCR Primer, Index 1 (95% over 22bp)
CCTTTTCCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAG7968922.0550749100247763No Hit
CCTCCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAG7908822.039575946289102RNA PCR Primer, Index 1 (95% over 22bp)
CCTTTCCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGG4662671.2024384898738127Illumina Small RNA Adapter 2 (100% over 21bp)
CCCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAG4325091.1153812489771586No Hit
CCTCACGTTCTAGCATTCAAGGTCCCATGGAATTCTCGGGTGCCAAGGAG3766900.971431722061751RNA PCR Primer, Index 1 (95% over 22bp)
CCAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGGA3593710.9267684021053215RNA PCR Primer, Index 1 (100% over 22bp)
CCCACGTTCTAGCATTCAAGGTCCCATGGAATTCTCGGGTGCCAAGGAGA3238890.83526520222692RNA PCR Primer, Index 1 (95% over 22bp)
CCTCACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGCCAAGGAGAT2939960.75817526496394RNA PCR Primer, Index 1 (95% over 22bp)
CCTTTTCCACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGCCAAGG2939900.7581597917888295Illumina Small RNA Adapter 2 (100% over 21bp)
CCAAGAGGTGCACAATCGACCGATCCTGTGGAATTCTCGGGTGCCAAGGA2885030.7440095731502864RNA PCR Primer, Index 1 (100% over 22bp)
CCCACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGCCAAGGAGATC2858580.7371884817890787RNA PCR Primer, Index 1 (95% over 22bp)
CCCCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAGA2618680.6753215699723025RNA PCR Primer, Index 1 (95% over 22bp)
CCTTCCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGA1797790.4636253246981325RNA PCR Primer, Index 1 (100% over 22bp)
CCTCCACGTTCTAGCATTCAAGGTCCCATGGAATTCTCGGGTGCCAAGGA1776040.45801629872057986RNA PCR Primer, Index 1 (100% over 22bp)
CCCCAACGGGGCCTTTCCTTCTGGCTGGAATTCTCGGGTGCCAAGGAGAT1751920.45179608232616286RNA PCR Primer, Index 1 (95% over 22bp)
CCTTTTCCACGTTCTAGCATTCAAGGTCCCATGGAATTCTCGGGTGCCAA1746340.4503570770408873No Hit
CCTGCCTTGTTGTAGACGGCCTTGGTGGAATTCTCGGGTGCCAAGGAGAT1702640.4390874478354137RNA PCR Primer, Index 1 (95% over 22bp)
CCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTG1616130.41677770818860543No Hit
CCTCCACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGCCAAGGAGA1528820.39426165954032394RNA PCR Primer, Index 1 (95% over 22bp)
CCTGCTCTGCTAGGCGGACTACTTGCGTGCCTGGAATTCTCGGGTGCCAA1448040.37342960811656745No Hit
CCTGCTCTGCTAGGCGGACTACTTGCGTGCCTTGGAATTCTCGGGTGCCA1418500.36581164823716955No Hit
CCTTTCCACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGCCAAGGA1411950.3641224932876077RNA PCR Primer, Index 1 (100% over 22bp)
CCAAATATAGATGGATACGAATAAGGCGTCCTGGAATTCTCGGGTGCCAA1397470.3603883003609427No Hit
CCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAGATC1331210.3433007573139249RNA PCR Primer, Index 1 (95% over 22bp)
CCTTGCCTTGTTGTAGACGGCCTTGGTGGAATTCTCGGGTGCCAAGGAGA1318530.34003075964057466RNA PCR Primer, Index 1 (95% over 22bp)
CCGCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAGA1226280.3162407529081962RNA PCR Primer, Index 1 (95% over 22bp)
CCCCCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAG1178270.3038596339572857RNA PCR Primer, Index 1 (95% over 22bp)
CCTTTCCACGTTCTAGCATTCAAGGTCCCATGGAATTCTCGGGTGCCAAG1056820.27253934867113533No Hit
CCTCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAA1049590.2706748310703213No Hit
CCTGCCTTGTTGTAGACGGCCTTGGTTGGAATTCTCGGGTGCCAAGGAGA1023500.26394657875977656RNA PCR Primer, Index 1 (95% over 22bp)
CCAAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAG995570.2567438157458434No Hit
CCTGTTGTAGACGGCCTTGGTGGAATTCTCGGGTGCCAAGGAGATCGGAA985900.2542500556905361RNA PCR Primer, Index 1 (95% over 22bp)
CCGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGCCA972640.2508304839911178No Hit
CCTTGCCTTGTTGTAGACGGCCTTGGTTGGAATTCTCGGGTGCCAAGGAG881880.22742472777398312RNA PCR Primer, Index 1 (95% over 22bp)
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT829140.21382380685186234No Hit
CCAGAGATGTATTTATTAGATTGGAATTCTCGGGTGCCAAGGAGATCGGA825060.21277163094434903RNA PCR Primer, Index 1 (95% over 22bp)
CCATATAGATGGATACGAATAAGGCGTCCTGGAATTCTCGGGTGCCAAGG806720.2080419970855759Illumina Small RNA Adapter 2 (100% over 21bp)
CCTATAGATGGATACGAATAAGGCGTCCTGGAATTCTCGGGTGCCAAGGA798230.20585254280744156RNA PCR Primer, Index 1 (100% over 22bp)
CCTGTTGTAGACGGCCTTGGTTGGAATTCTCGGGTGCCAAGGAGATCGGA793970.20475394737459676RNA PCR Primer, Index 1 (95% over 22bp)
CCCCAACGGGGCCTTTCCTTCTGGCTAACCTTGGAATTCTCGGGTGCCAA677190.17463799088454623No Hit
CCTAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAG673870.17378180852843245No Hit
CCACTATGCCGACTAGGGATCGGGTGGTGTGGAATTCTCGGGTGCCAAGG656600.16932811295912972Illumina Small RNA Adapter 2 (100% over 21bp)
CCAAATATAGATGGATACGAATAAGGCGTCTGGAATTCTCGGGTGCCAAG627160.16173594170491593No Hit
CCCCACGTTCTAGCATTCAAGGTCCCATGGAATTCTCGGGTGCCAAGGAG623450.16077918371058397RNA PCR Primer, Index 1 (95% over 22bp)
CCTAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG609040.15706304282154793Illumina Small RNA Adapter 2 (100% over 21bp)
CCTCAATATAGATGGATACGAATAAGGCGTCCTGGAATTCTCGGGTGCCA588740.15182795190916545No Hit
CCTGCGTGCCTTGTTGTAGACGGCCTTGGTGGAATTCTCGGGTGCCAAGG586430.1512322346674116Illumina Small RNA Adapter 2 (100% over 21bp)
CCAAGAGGTGCACAATCGACCGATCCTGATTGGAATTCTCGGGTGCCAAG568670.14665217483470652No Hit
CCCAATATAGATGGATACGAATAAGGCGTCCTGGAATTCTCGGGTGCCAA562240.14499396623536567No Hit
CCCAAGAGGTGCACAATCGACCGATCCTGTGGAATTCTCGGGTGCCAAGG528230.1362232548102362Illumina Small RNA Adapter 2 (100% over 21bp)
CCTTCCACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGCCAAGGAG528010.13616651983483108RNA PCR Primer, Index 1 (95% over 22bp)
CCAACTATGCCGACTAGGGATCGGGTGGTGTGGAATTCTCGGGTGCCAAG508760.13120220948688216No Hit
CCCCACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGCCAAGGAGAT506140.13052654750705744RNA PCR Primer, Index 1 (95% over 22bp)
CCAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAA501210.12925516828547884No Hit
CCGCCAAGGACGTTTTCATTAATCAAGAACGTGGAATTCTCGGGTGCCAA492720.12706571400734448No Hit
CCTTGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGC491280.12669435780469274No Hit
CCTTTTCCACGTTCTAGCATTCAAGGTCTGGAATTCTCGGGTGCCAAGGA487680.12576596729806333RNA PCR Primer, Index 1 (100% over 22bp)
CCGCTCTGCTAGGCGGACTACTTGCGTGCCTTGGAATTCTCGGGTGCCAA466650.12034261942183656No Hit
CCAGAGGTGCACAATCGACCGATCCTGTGGAATTCTCGGGTGCCAAGGAG462400.11924660285151019RNA PCR Primer, Index 1 (95% over 22bp)
CCGCCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAG462310.11922339308884444RNA PCR Primer, Index 1 (95% over 22bp)
CCAAGAGGTGTACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG456870.11782049187882668Illumina Small RNA Adapter 2 (100% over 21bp)
CCAAGAGGTACACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG444160.11454275761792121Illumina Small RNA Adapter 2 (100% over 21bp)
CCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGTCAAGG441610.11388514767572537Illumina Small RNA Adapter 2 (95% over 21bp)
CCGCTCTGCTAGGCGGACTACTTGCGTGCCTGGAATTCTCGGGTGCCAAG437710.1128793912935435No Hit
CCAATATAGATGGATACGAATAAGGCGTCCTGGAATTCTCGGGTGCCAAG424090.10936698054346226No Hit
CCTCAACGGGGCCTTTCCTTCTGGCTGGAATTCTCGGGTGCCAAGGAGAT411390.1060918251450752RNA PCR Primer, Index 1 (95% over 22bp)
TCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG403430.10403905058041685Illumina Small RNA Adapter 2 (100% over 21bp)
CCTTCCACGTTCTAGCATTCAAGGTCCCATGGAATTCTCGGGTGCCAAGG395460.10198369715324009Illumina Small RNA Adapter 2 (100% over 21bp)
CCACACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAGA391360.10092636352068993RNA PCR Primer, Index 1 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)