FastQCFastQC Report
Tue 4 Jul 2023
SRR6075751_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6075751_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38458826
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length30-70
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTG34031758.84887905834671No Hit
CCTCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAGA21961725.710449923770424RNA PCR Primer, Index 1 (95% over 22bp)
CCCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAGAT21511705.5934364715137175RNA PCR Primer, Index 1 (95% over 22bp)
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT16216634.216621172991604No Hit
CCTATAGATGGATACGAATAAGGCGTCTGGAATTCTCGGGTGCCAAGGAG10165742.6432788145951203RNA PCR Primer, Index 1 (95% over 22bp)
CCAGAGGTGCACAATCGACCGATCCTGTGGAATTCTCGGGTGCCAAGGAG8188552.1291731578077813RNA PCR Primer, Index 1 (95% over 22bp)
CCAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGGA7714192.005830859215515RNA PCR Primer, Index 1 (100% over 22bp)
CCCACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGCCAAGGAGATC7703172.002965457135899RNA PCR Primer, Index 1 (95% over 22bp)
CCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAGATC7591221.9738564042490532RNA PCR Primer, Index 1 (95% over 22bp)
CCTCACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGCCAAGGAGAT7449671.9370508085712237RNA PCR Primer, Index 1 (95% over 22bp)
CCATATAGATGGATACGAATAAGGCGTCTGGAATTCTCGGGTGCCAAGGA5937781.5439316842380992RNA PCR Primer, Index 1 (100% over 22bp)
CCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG5089791.3234387341932903Illumina Small RNA Adapter 2 (100% over 21bp)
CCAAGAGGTGCACAATCGACCGATCCTGTGGAATTCTCGGGTGCCAAGGA4807781.2501109628255422RNA PCR Primer, Index 1 (100% over 22bp)
CCAAATATAGATGGATACGAATAAGGCGTCTGGAATTCTCGGGTGCCAAG4089561.0633605924424214No Hit
CCTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTGC3055310.7944366268486719No Hit
CCTAGATGGATACGAATAAGGCGTCTGGAATTCTCGGGTGCCAAGGAGAT2709050.7044026772944135RNA PCR Primer, Index 1 (95% over 22bp)
CCTTGCTCGAATATATTAGCATGGAATAATAGAATATGGAATTCTCGGGT2658820.6913419562001191No Hit
CCACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGCCAAGGAGATCG2330200.605894730119947RNA PCR Primer, Index 1 (95% over 22bp)
CCCCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAGA2148460.5586389974566566RNA PCR Primer, Index 1 (95% over 22bp)
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG1918080.49873597285574967No Hit
CCGCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAGA1792770.46615307497945985RNA PCR Primer, Index 1 (95% over 22bp)
CCTTATAGATGGATACGAATAAGGCGTCTGGAATTCTCGGGTGCCAAGGA1767720.4596396156242523RNA PCR Primer, Index 1 (100% over 22bp)
CCTTGCTCGAATATATTAGCATGGAATTCTCGGGTGCCAAGGAGATCGGA1478560.38445271314314167RNA PCR Primer, Index 1 (95% over 22bp)
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG1472560.3828926031179423No Hit
CCAATATAGATGGATACGAATAAGGCGTCTGGAATTCTCGGGTGCCAAGG1335060.3471400817071223Illumina Small RNA Adapter 2 (100% over 21bp)
CCTATAGATGGATACGAATAAGGCGTCCTGGAATTCTCGGGTGCCAAGGA1282020.3333487090843595RNA PCR Primer, Index 1 (100% over 22bp)
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATATGGAATTCTCGGG1263300.3284811658057373No Hit
CCAGATGGATACGAATAAGGCGTCTGGAATTCTCGGGTGCCAAGGAGATC1182470.3074638835829258RNA PCR Primer, Index 1 (95% over 22bp)
CCCACGTTCTAGCATTCAAGGTCTGGAATTCTCGGGTGCCAAGGAGATCG1073070.27901787745678974RNA PCR Primer, Index 1 (95% over 22bp)
CCTCACGTTCTAGCATTCAAGGTCCCATGGAATTCTCGGGTGCCAAGGAG1062340.27622788069505816RNA PCR Primer, Index 1 (95% over 22bp)
CCTTGTGGCGTCGCTGAACCATATGGAATTCTCGGGTGCCAAGGAGATCG1006730.26176826094483485RNA PCR Primer, Index 1 (95% over 22bp)
CCTCACGTTCTAGCATTCAAGGTCTGGAATTCTCGGGTGCCAAGGAGATC986070.2563962820913982RNA PCR Primer, Index 1 (95% over 22bp)
CCCACGTTCTAGCATTCAAGGTCCCATGGAATTCTCGGGTGCCAAGGAGA984750.2560530578858543RNA PCR Primer, Index 1 (95% over 22bp)
CCTAATATAGATGGATACGAATAAGGCGTCTGGAATTCTCGGGTGCCAAG838110.21792396886998058No Hit
CCATATAGATGGATACGAATAAGGCGTCCTGGAATTCTCGGGTGCCAAGG785420.2042236026653544Illumina Small RNA Adapter 2 (100% over 21bp)
CCCCACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGCCAAGGAGAT740270.19248377472572875RNA PCR Primer, Index 1 (95% over 22bp)
CCTATATAGATGGATACGAATAAGGCGTCTGGAATTCTCGGGTGCCAAGG737100.1916595165957484Illumina Small RNA Adapter 2 (100% over 21bp)
CCCAATATAGATGGATACGAATAAGGCGTCTGGAATTCTCGGGTGCCAAG731800.19028141940682225No Hit
CCCATAGATGGATACGAATAAGGCGTCTGGAATTCTCGGGTGCCAAGGAG726410.18887992056751812RNA PCR Primer, Index 1 (95% over 22bp)
CCCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT679570.17670066163746131No Hit
CCACGTTCTAGCATTCAAGGTCTGGAATTCTCGGGTGCCAAGGAGATCGG657060.17084764885958817RNA PCR Primer, Index 1 (95% over 22bp)
CCTGAGGTGCACAATCGACCGATCCTGTGGAATTCTCGGGTGCCAAGGAG644930.16769362642531002RNA PCR Primer, Index 1 (95% over 22bp)
CCTGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGGA634760.165049239932597RNA PCR Primer, Index 1 (100% over 22bp)
CCGTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT633200.16464361132604516No Hit
CCTCAATATAGATGGATACGAATAAGGCGTCTGGAATTCTCGGGTGCCAA630420.16392076034770275No Hit
CCGCACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGCCAAGGAGAT625010.16251406114164796RNA PCR Primer, Index 1 (95% over 22bp)
CCTGTGGCGTCGCTGAACCATATGGAATTCTCGGGTGCCAAGGAGATCGG622360.16182501254718487RNA PCR Primer, Index 1 (95% over 22bp)
CCGGTTGTTTGGCCGAGCGGTGGAATTCTCGGGTGCCAAGGAGATCGGAA606390.1576725196967791RNA PCR Primer, Index 1 (95% over 22bp)
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT598170.1555351689622559Illumina Paired End PCR Primer 2 (100% over 27bp)
CCGATAGATGGATACGAATAAGGCGTCTGGAATTCTCGGGTGCCAAGGAG597170.15527515062472266RNA PCR Primer, Index 1 (95% over 22bp)
CCATAGATGGATACGAATAAGGCGTCTGGAATTCTCGGGTGCCAAGGAGA595750.15490592458542546RNA PCR Primer, Index 1 (95% over 22bp)
CCAATAGATGGATACGAATAAGGCGTCTGGAATTCTCGGGTGCCAAGGAG573110.149019109423673RNA PCR Primer, Index 1 (95% over 22bp)
CCACACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAGA559390.145451657832717RNA PCR Primer, Index 1 (95% over 22bp)
CCTAGAGGTGCACAATCGACCGATCCTGTGGAATTCTCGGGTGCCAAGGA557710.14501482702566115RNA PCR Primer, Index 1 (100% over 22bp)
CCAAATATAGATGGATACGAATAAGGCGTCCTGGAATTCTCGGGTGCCAA555810.144520792184348No Hit
CCTAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG546210.14202461614402892Illumina Small RNA Adapter 2 (100% over 21bp)
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGC531900.13830375373392834No Hit
CCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAAGGAGAT498920.12972834896208219RNA PCR Primer, Index 1 (95% over 22bp)
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT498460.1296087405268169No Hit
CCTTCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAG480880.12503761815298262RNA PCR Primer, Index 1 (95% over 22bp)
CCTTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAAGGAGA470630.12237243019326695RNA PCR Primer, Index 1 (95% over 22bp)
CCCGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAGATCG445070.11572636148591744RNA PCR Primer, Index 1 (95% over 22bp)
CCAGAGGTGCACAATCGACCGATCCTGGAATTCTCGGGTGCCAAGGAGAT419690.10912709607932389RNA PCR Primer, Index 1 (95% over 22bp)
CCGTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTG406750.10576245879164382No Hit
CCTGTGCCCGAGTTGTAATTTGTGGAATTCTCGGGTGCCAAGGAGATCGG396810.10317787651656346RNA PCR Primer, Index 1 (95% over 22bp)
CCTAGATGGATACGAATAAGGCGTCCTGGAATTCTCGGGTGCCAAGGAGA395540.10284765322789624RNA PCR Primer, Index 1 (95% over 22bp)
CCTTTGCTCGAATATATTAGCATGGAATTCTCGGGTGCCAAGGAGATCGG388890.10111853128330023RNA PCR Primer, Index 1 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)