FastQCFastQC Report
Tue 4 Jul 2023
SRR6075752_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6075752_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38123734
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length30-70
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAGAT16182014.2446025879836435RNA PCR Primer, Index 1 (95% over 22bp)
CCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG16054664.211198200050394Illumina Small RNA Adapter 2 (100% over 21bp)
CCTCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAGA12596383.304078241653874RNA PCR Primer, Index 1 (95% over 22bp)
CCTCCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAG5887671.5443581680640202RNA PCR Primer, Index 1 (95% over 22bp)
CCTTTTCCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAG5520571.4480664459572612No Hit
CCCACGTTCTAGCATTCAAGGTCCCATGGAATTCTCGGGTGCCAAGGAGA5169281.3559217468047593RNA PCR Primer, Index 1 (95% over 22bp)
CCTCACGTTCTAGCATTCAAGGTCCCATGGAATTCTCGGGTGCCAAGGAG4259871.1173800551645858RNA PCR Primer, Index 1 (95% over 22bp)
CCCACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGCCAAGGAGATC3703410.9714184869719213RNA PCR Primer, Index 1 (95% over 22bp)
CCTTTCCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGG3602650.944988756872556Illumina Small RNA Adapter 2 (100% over 21bp)
CCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTG3337970.8755621891601698No Hit
CCCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAG3174340.8326414196468793No Hit
CCTCACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGCCAAGGAGAT2726650.715210634928887RNA PCR Primer, Index 1 (95% over 22bp)
CCAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGGA2621980.6877552970021247RNA PCR Primer, Index 1 (100% over 22bp)
CCTGCCTTGTTGTAGACGGCCTTGGTGGAATTCTCGGGTGCCAAGGAGAT2446710.6417813113479388RNA PCR Primer, Index 1 (95% over 22bp)
CCAAGAGGTGCACAATCGACCGATCCTGTGGAATTCTCGGGTGCCAAGGA2292120.6012317681158934RNA PCR Primer, Index 1 (100% over 22bp)
CCCCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAGA2288910.6003897729430177RNA PCR Primer, Index 1 (95% over 22bp)
CCTGTTGTAGACGGCCTTGGTGGAATTCTCGGGTGCCAAGGAGATCGGAA2281190.5983647876674411RNA PCR Primer, Index 1 (95% over 22bp)
CCTTTTCCACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGCCAAGG2151650.5643859544293327Illumina Small RNA Adapter 2 (100% over 21bp)
CCCCAACGGGGCCTTTCCTTCTGGCTGGAATTCTCGGGTGCCAAGGAGAT2006740.5263755119055232RNA PCR Primer, Index 1 (95% over 22bp)
CCTCCACGTTCTAGCATTCAAGGTCCCATGGAATTCTCGGGTGCCAAGGA1905550.4998329911755234RNA PCR Primer, Index 1 (100% over 22bp)
CCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAGATC1818980.47712535188709476RNA PCR Primer, Index 1 (95% over 22bp)
CCTTCCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGA1713550.4494706630782808RNA PCR Primer, Index 1 (100% over 22bp)
CCTTTTCCACGTTCTAGCATTCAAGGTCCCATGGAATTCTCGGGTGCCAA1680580.44082250705033243No Hit
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT1429850.3750550772387616Illumina Paired End PCR Primer 2 (100% over 27bp)
CCGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGCCA1411070.37012901202175No Hit
CCTTGCCTTGTTGTAGACGGCCTTGGTGGAATTCTCGGGTGCCAAGGAGA1308760.34329271104451625RNA PCR Primer, Index 1 (95% over 22bp)
CCTGCCTTGTTGTAGACGGCCTTGGTTGGAATTCTCGGGTGCCAAGGAGA1267180.3323861193659572RNA PCR Primer, Index 1 (95% over 22bp)
CCTCCACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGCCAAGGAGA1229410.32247890513557775RNA PCR Primer, Index 1 (95% over 22bp)
CCTGCTCTGCTAGGCGGACTACTTGCGTGCCTTGGAATTCTCGGGTGCCA1212400.31801711763071266No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT1196810.3139278015107334No Hit
CCTTTCCACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGCCAAGGA1150380.3017490364401347RNA PCR Primer, Index 1 (100% over 22bp)
CCTTTCCACGTTCTAGCATTCAAGGTCCCATGGAATTCTCGGGTGCCAAG1148280.30119819847657103No Hit
CCTGCTCTGCTAGGCGGACTACTTGCGTGCCTGGAATTCTCGGGTGCCAA1117590.2931480950947774No Hit
CCTGTTGTAGACGGCCTTGGTTGGAATTCTCGGGTGCCAAGGAGATCGGA1087680.2853025886708789RNA PCR Primer, Index 1 (95% over 22bp)
CCAGAGATGTATTTATTAGATTGGAATTCTCGGGTGCCAAGGAGATCGGA1079440.28314120542337223RNA PCR Primer, Index 1 (95% over 22bp)
CCAAATATAGATGGATACGAATAAGGCGTCCTGGAATTCTCGGGTGCCAA1035940.2717309904638407No Hit
CCGCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAGA995460.2611129329566721RNA PCR Primer, Index 1 (95% over 22bp)
CCTTGCTCGAATATATTAGCATGGAATAATAGAATATGGAATTCTCGGGT988600.2593135289423643No Hit
CCACTATGCCGACTAGGGATCGGGTGGTGTGGAATTCTCGGGTGCCAAGG860270.22565208329278552Illumina Small RNA Adapter 2 (100% over 21bp)
CCCCCACGTTCTAGCATTCAAGGTCCCTGGAATTCTCGGGTGCCAAGGAG751940.19723671348666946RNA PCR Primer, Index 1 (95% over 22bp)
CCCCACGTTCTAGCATTCAAGGTCCCATGGAATTCTCGGGTGCCAAGGAG748140.19623995907641156RNA PCR Primer, Index 1 (95% over 22bp)
CCTTGCCTTGTTGTAGACGGCCTTGGTTGGAATTCTCGGGTGCCAAGGAG736130.19308969053241218RNA PCR Primer, Index 1 (95% over 22bp)
CCTGCGTGCCTTGTTGTAGACGGCCTTGGTGGAATTCTCGGGTGCCAAGG702380.18423693754656875Illumina Small RNA Adapter 2 (100% over 21bp)
CCATGATTAATAGGGACGGTCGGGGGCTGGAATTCTCGGGTGCCAAGGAG686860.18016598269204168RNA PCR Primer, Index 1 (95% over 22bp)
CCAACTATGCCGACTAGGGATCGGGTGGTGTGGAATTCTCGGGTGCCAAG684300.17949448498407844No Hit
CCGCCAAGGACGTTTTCATTAATCAAGAACGTGGAATTCTCGGGTGCCAA684090.17943940118772206No Hit
CCAAATATAGATGGATACGAATAAGGCGTCTGGAATTCTCGGGTGCCAAG669980.17573829468015909No Hit
CCAGAGGTGCACAATCGACCGATCCTGTGGAATTCTCGGGTGCCAAGGAG663850.17413037243413776RNA PCR Primer, Index 1 (95% over 22bp)
CCATATAGATGGATACGAATAAGGCGTCCTGGAATTCTCGGGTGCCAAGG658540.1727375392976984Illumina Small RNA Adapter 2 (100% over 21bp)
CCTATAGATGGATACGAATAAGGCGTCCTGGAATTCTCGGGTGCCAAGGA653250.17134995223710248RNA PCR Primer, Index 1 (100% over 22bp)
CCTTGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGC651250.17082534465275623No Hit
CCCAATATAGATGGATACGAATAAGGCGTCCTGGAATTCTCGGGTGCCAA647400.16981547505288963No Hit
CCTATAGATGGATACGAATAAGGCGTCTGGAATTCTCGGGTGCCAAGGAG635210.16661799182629908RNA PCR Primer, Index 1 (95% over 22bp)
CCAAGAGGTGCACAATCGACCGATCCTTGGAATTCTCGGGTGCCAAGGAG625780.16414446706610636RNA PCR Primer, Index 1 (95% over 22bp)
CCCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTTGGAATTCTCGGGT589030.15450480270374356No Hit
CCTGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGCC577020.15135453415974415No Hit
CCCCAACGGGGCCTTTCCTTCTGGCTAACCTTGGAATTCTCGGGTGCCAA561460.14727308715353013No Hit
CCTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCCA547640.14364804874569737No Hit
CCAAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAG547480.14360608013894968No Hit
CCAACTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCGGGTGCCAA546990.14347755128078482No Hit
CCTTCCACGTTCTAGCATTCAAGGTCCCATGGAATTCTCGGGTGCCAAGG533230.13986825110048245Illumina Small RNA Adapter 2 (100% over 21bp)
CCGCTCTGCTAGGCGGACTACTTGCGTGCCTTGGAATTCTCGGGTGCCAA529010.1387613290975118No Hit
CCTCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAA525930.13795343341761854No Hit
CCTTCCACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGCCAAGGAG524970.13770162177713233RNA PCR Primer, Index 1 (95% over 22bp)
CCACTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCGGGTGCCAAG521330.1367468359736221No Hit
CCACGTTCTAGCATTCAAGGTCCCATGGAATTCTCGGGTGCCAAGGAGAT518080.1358943486490594RNA PCR Primer, Index 1 (95% over 22bp)
CCATATAGATGGATACGAATAAGGCGTCTGGAATTCTCGGGTGCCAAGGA497710.1305512204024926RNA PCR Primer, Index 1 (100% over 22bp)
CCTCAACGGGGCCTTTCCTTCTGGCTGGAATTCTCGGGTGCCAAGGAGAT496230.13016301079007633RNA PCR Primer, Index 1 (95% over 22bp)
CCGTTGTAGACGGCCTTGGTTGGAATTCTCGGGTGCCAAGGAGATCGGAA495500.12997152902178996RNA PCR Primer, Index 1 (95% over 22bp)
CCGAAAAGAAACCAACCGGGATTGCCTTAGTAACGTGGAATTCTCGGGTG484370.12705208781490293No Hit
CCTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTGC481210.1262232078316358No Hit
CCCCACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGCCAAGGAGAT480590.12606057948048846RNA PCR Primer, Index 1 (95% over 22bp)
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG476200.12490906583284837No Hit
CCAAGAGGTGCACAATCGACCGATCCTGATTGGAATTCTCGGGTGCCAAG472270.12387821192960795No Hit
CCTCAATATAGATGGATACGAATAAGGCGTCCTGGAATTCTCGGGTGCCA462460.12130501172838946No Hit
CCAATATAGATGGATACGAATAAGGCGTCCTGGAATTCTCGGGTGCCAAG457500.1200039849192107No Hit
CCGCTCTGCTAGGCGGACTACTTGCGTGCCTGGAATTCTCGGGTGCCAAG447420.11735996269410547No Hit
CCCAAGAGGTGCACAATCGACCGATCCTGTGGAATTCTCGGGTGCCAAGG423960.11120631572972364Illumina Small RNA Adapter 2 (100% over 21bp)
CCACGTTCTAGCATTCAAGGTCCTGGAATTCTCGGGTGCCAAGGAGATCG418480.10976889094861483RNA PCR Primer, Index 1 (95% over 22bp)
CCTGAGCGGAGAATTTGGACAAACTTGGTCATTTGGAATTCTCGGGTGCC393220.10314309715832137No Hit
CCCTATGCCGACTAGGGATCGGGTGGTGTGGAATTCTCGGGTGCCAAGGA388030.10178174047694279RNA PCR Primer, Index 1 (100% over 22bp)
CCCGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGCTGGAATTCTCGGGTG387410.10161911212579544No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)