FastQCFastQC Report
Mon 25 Sept 2023
SRR609197.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR609197.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23804050
Total Bases952.1 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAA6970512.9282874132763124Illumina Single End Adapter 1 (95% over 22bp)
TGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAA5292762.223470375839405Illumina Single End Adapter 1 (95% over 22bp)
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGC2787721.1711116385657063No Hit
ACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTG2336080.9813792190824671Illumina Single End Adapter 1 (95% over 22bp)
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCA1759140.739008698099693No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGC1694430.7118242483947059No Hit
GTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAA1618310.6798464967095935Illumina Single End Adapter 1 (95% over 22bp)
TAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAA1606280.6747927348497419Illumina Single End Adapter 1 (95% over 22bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGG1334510.5606230872477582No Hit
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGA1142700.48004436219886953Illumina Single End Adapter 1 (95% over 22bp)
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCAC795030.33398938415941826No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGC788270.3311495312772406No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCAC741620.3115520258107339No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCA736780.30951875836254755No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTA592420.2488736160443286No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGT569990.2394508497503576No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACC563500.23672442294483503No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGC540980.22726384795864568No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCA500320.21018272100755964No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGG453840.19065663195968754No Hit
TAGTGACGAAAAATAACAATACAGGACTGTAGGCACCATC440350.18498952909273841No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGG415210.1744283010664152No Hit
GGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCAT410300.17236562685761458No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACC384890.1616909727546363No Hit
AGGATAGCTGGCGCTCTCGCAGACCCGACTGTAGGCACCA368070.15462494827560858No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGC368030.15460814441240042No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCA362630.15233962287929995No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCAC355280.1492519130148021No Hit
AGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAA350950.1474328948225197Illumina Single End Adapter 1 (95% over 22bp)
CCAACTTCTTAGAGGGACAAGTGGCGTTCTGTAGGCACCA341930.14364362366908154No Hit
AAGTCAGAATCCCGCCCAGGCGGAACGATACCTGTAGGCA339920.1427992295428719No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGG321720.13515347178316295No Hit
AGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCA320190.13451072401545117No Hit
AAAATCAGTTATGGTTCCTTTGGTCGCTCCTGTAGGCACC319320.13414523999067385No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTA319070.13404021584562292No Hit
AAAATCAGTTATGGTTCCTTTGGTCGCTCGCTGTAGGCAC318440.1337755550000945No Hit
AGGGGAATCCGACTGTTTAATTAAAACCTGTAGGCACCAT313970.13189772328658358No Hit
TGTGCATTTATCAGATCAAAACCAACCCGGCTGTAGGCAC310090.1302677485553929No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTA303710.12758753237369272No Hit
CTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCA299800.1259449547450959No Hit
AGGAGGTGTCAGAAAAGTTACCACAGGGATCTGTAGGCAC292180.12274381880394303No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCCTGTAGGCA290220.1219204295067436No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACC289630.12167257252442337No Hit
ACGATCCTTCTGACCTTTTGGGTTTTAAGCCTGTAGGCAC277140.11642556623767805No Hit
CCCTGTAATTGGAATGAGTCCACTTTCTGTAGGCACCATC273570.11492582144635051No Hit
TAGACGTGGCGACCCGCTGAATTTAAGCCTGTAGGCACCA261730.10995187793673765No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCTGTAGGCACCA254990.10712042698616411No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGG254610.10696079028568667No Hit
AGCGTTTACTTTGAAAAAATTAGAGTGTTCCTGTAGGCAC254250.1068095555168133No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAG247510.10397810456623978No Hit
TAGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACC246200.10342777804617281No Hit
TCACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACC245080.10295726987634456No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGC239690.10069294930904614No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)