FastQCFastQC Report
Thu 28 Sept 2023
SRR6107148.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6107148.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56564807
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCGGATAGCTCAGTCGGTAGAGCATCAGATGGAATTCTCGGGTGCCAA42821147.570279520267788No Hit
CCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC35808856.330588204782525RNA PCR Primer, Index 1 (100% over 31bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATGGAATTCTCGGGTGCCAA34268336.058242185817058No Hit
ACCCCCCTCGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTT8722001.541948158684604RNA PCR Primer, Index 12 (100% over 36bp)
CCCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT8032691.4200861677120191RNA PCR Primer, Index 1 (100% over 30bp)
CGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA6624501.1711345536810547RNA PCR Primer, Index 1 (100% over 32bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGGAATTCTCGGGTGCCA6011331.0627332291613758No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA5907051.0442977379910445No Hit
CCGCGAGGGGGGCCCGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA5826091.0299849515972006RNA PCR Primer, Index 1 (100% over 32bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG5281440.9336971661549204No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTATGC5175130.9149027945945258RNA PCR Primer, Index 12 (100% over 50bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGTGGAATTCTCGGGTGCCAAG5097430.9011663382852168No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG4632830.8190304618205451No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCA4129600.7300652506425064No Hit
ACCCCCCTCGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTG3822230.675725809512618RNA PCR Primer, Index 12 (100% over 37bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGATGGAATTCTCGGGTGCC2872520.5078281271250514No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGATGGAATTCTCGGGTGC2852240.5042428589918109No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGTGGAATTCTCGGGTGCCAAG2710130.47911946380370396No Hit
CGGCGGGCCGCCCCCCCCTCCACGCGCTGGAATTCTCGGGTGCCAAGGAA2569750.4543019124948132RNA PCR Primer, Index 1 (100% over 23bp)
CCCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCACGGAACTCCAGT2421100.42802232137024704RNA PCR Primer, Index 1 (96% over 30bp)
GCCCGGATAGCTCAGTCGGTAGAGCATGGAATTCTCGGGTGCCAAGGAAC2340280.4137342853481317RNA PCR Primer, Index 1 (100% over 24bp)
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA2312620.4088443190480611No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGATGGAATTCTCGGGTGC2021120.3573105093419659No Hit
CGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTGGAATTCTCGGGT1798230.31790614966652325No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA1744140.3083436667608536No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGATGGAATTCTCGGGTGCC1637690.2895245448287307No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGATGGAATTCTCGGGTGCCA1610800.28477070557316675No Hit
CGCGGCTGGACGAGGCGCCGCCGCCCCCCCCTGGAATTCTCGGGTGCCAA1489750.26337047344650183No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAA1472200.26026783756196675No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTCATCTCGTATGC1426670.2522186630991245RNA PCR Primer, Index 12 (98% over 50bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG1380180.24399977180157265Illumina Small RNA Adapter 2 (100% over 21bp)
CGGCGGGCCGCCCCCCCCCCTCCACGCGCTGGAATTCTCGGGTGCCAAGG1356270.23977276188708643Illumina Small RNA Adapter 2 (100% over 21bp)
CACTGCACTCCAGCCTGGGCAACATAGCGATGGAATTCTCGGGTGCCAAG1309390.2314849231254338No Hit
CACTGCACTCCAGCCTGGGCAACATAGCGTGGAATTCTCGGGTGCCAAGG1248000.2206318851224932Illumina Small RNA Adapter 2 (100% over 21bp)
CGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTGGAATTCTCGGGTGCC1238630.21897537810037965No Hit
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA1237150.2187137313135356No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA1215170.21482792295216352No Hit
CGGCGGCGGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTG1210990.21408894756769878RNA PCR Primer, Index 12 (100% over 37bp)
CCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA1191520.2106468780137445No Hit
GCCCTAGCTCAGTCGGTAGAGCATGAGATGGAATTCTCGGGTGCCAAGGA1087750.19230154891185255RNA PCR Primer, Index 1 (100% over 22bp)
CGCGAGGGGGGCCCGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1064630.18821420180926277RNA PCR Primer, Index 1 (100% over 33bp)
CGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCTGGAATTCTCGGGTG1038800.18364775822535734No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGATGGAATTCTCGGGTGCC1026430.18146088609477623No Hit
TCCCTGGTGGTCTAGTGGCTAGGATTCGGCGCTGGAATTCTCGGGTGCCA1017560.1798927732574072No Hit
TCCTCCTCGGCGCGCGGCGGCGGTGGAATTCTCGGGTGCCAAGGAACTCC978220.17293791880170298RNA PCR Primer, Index 1 (100% over 27bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCC931440.16466775887699928No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA912390.16129994043823043No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA905430.16006949338658577No Hit
CGGGGGTGTGGGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTT895370.15829100238952462RNA PCR Primer, Index 12 (100% over 36bp)
GCCCGCATAGCTCAGTCGGTAGAGCATCAGATGGAATTCTCGGGTGCCAA877410.155115883273499No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTGGAATTCTCGGGTGCCA871400.15405338517286907No Hit
CCCCCCTCGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTG846340.14962306863347027RNA PCR Primer, Index 12 (100% over 37bp)
CGCCGCCGCCCCCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC818510.14470304831058647RNA PCR Primer, Index 2 (100% over 34bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGATGGAATTCTCGGGTGCC814440.14398351964676553No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGTGGAATTCTCGGGTG809270.14306952377650647No Hit
CGGCGGGCCGCCCCCCCCTCCACGCGCCTGGAATTCTCGGGTGCCAAGGA801860.14175952195859168RNA PCR Primer, Index 1 (100% over 22bp)
GCCCGAATAGCTCAGTCGGTAGAGCATCAGATGGAATTCTCGGGTGCCAA800810.14157389417062805No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGATGGACTTCTCGGGTGC798830.14122385319903946No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGATGGAATTCTCGGGTGCCAA789120.13950723813129956No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGAATTCTCGGGTGCCAAGGAAC771800.13644526357174702RNA PCR Primer, Index 1 (100% over 24bp)
AGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC764750.1351989055668483RNA PCR Primer, Index 1 (100% over 31bp)
GCCCGGATAGCTCAGTCGGTAGAGCTGGAATTCTCGGGTGCCAAGGAACT744960.13170026373465749RNA PCR Primer, Index 1 (100% over 25bp)
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCA720600.12739369905390113No Hit
CGGCGGCGGCGGCGACTCTGGACGCTGGAATTCTCGGGTGCCAAGGAACT718090.12694996024648328RNA PCR Primer, Index 1 (100% over 25bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAG715760.1265380433455735No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTTGGAATTCTCGGGTGCCAAG713230.1260907687707659No Hit
CGGCGGCGGCGGCGACTCTGGACGCGTGGAATTCTCGGGTGCCAAGGAAC700180.12378368054893213RNA PCR Primer, Index 1 (100% over 24bp)
AGCAGAGTCGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA671670.11874344413479569No Hit
TGCGAGAGGTCCCGGGTTCATCCCGGACGAGCTGGAATTCTCGGGTGCCA664930.11755189052443864No Hit
CCCCGGGGAGCCCGGCGGGCGCCGGCTGGAATTCTCGGGTGCCAAGGAAC660610.11678816476824538RNA PCR Primer, Index 1 (100% over 24bp)
GGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTGGAATTCTCGGGTGCCA647830.11452880940617371No Hit
CGGGGGTGTGGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTG637990.1127892118504002RNA PCR Primer, Index 12 (100% over 37bp)
TTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGGAA637760.1127485505254177RNA PCR Primer, Index 1 (100% over 23bp)
ATCCAGCGATCCGAGTTCATCTCGGTGGAACCTGGAATTCTCGGGTGCCA628160.1110513821783216No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG613530.10846496833269494No Hit
CGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTGGAATTCTCGGG595480.10527393826341527No Hit
CGGGTCGGGGCGGCGGCGGCGGCTGGAATTCTCGGGTGCCAAGGAACTCC593590.10493980824508073RNA PCR Primer, Index 1 (100% over 27bp)
AATGGTTAGCACTCTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA587490.10386139919119673RNA PCR Primer, Index 1 (100% over 32bp)
CCCCCGGGGAGCCCGGCGGGCGCCGGCTGGAATTCTCGGGTGCCAAGGAA579220.10239935937552125RNA PCR Primer, Index 1 (100% over 23bp)
GGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTGGAATTCTCGGGT574970.10164800880519224No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGATGGAATTCTCGGGTGCCAA572310.10117775174235105No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGATGGACTTCTCGGGTGC570830.10091610495550705No Hit
CGGGCGGCGGAGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC567520.10033093545249788RNA PCR Primer, Index 2 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)