FastQCFastQC Report
Thu 28 Sept 2023
SRR6107149.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6107149.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80033370
Total Bases4 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTCAAAGTGAAGAAATTCAATGAAGCTGGAATTCTCGGGTGCCAAGGA25505373.18684193855638RNA PCR Primer, Index 1 (100% over 22bp)
AGAATTCACCAAGCGTTGGATTGTTCACCCTGGAATTCTCGGGTGCCAAG15417311.9263602169944862No Hit
ATGTCAAAGTGAAGAAATTCAATGATGGAATTCTCGGGTGCCAAGGAACT14820771.8518238079940903RNA PCR Primer, Index 1 (100% over 25bp)
AGAATTCACCAAGCGTTGGATTGTTCACCCACTTGGAATTCTCGGGTGCC12449671.555559887082101No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATGGAATTCTCGGGTGCCAA11703691.4623512667278662No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGATGGAATTCTCGGGTGCCAA11411501.4258427453448481No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCTGGAATTCTCGGGTGCCAAGG9844821.230089398959459Illumina Small RNA Adapter 2 (100% over 21bp)
AGCAGAATTCACCAAGCGTTGGATTGTTCTGGAATTCTCGGGTGCCAAGG8527911.065544284840186Illumina Small RNA Adapter 2 (100% over 21bp)
TGTCGGCTCTTCCTATCATTGTGAAGCTGGAATTCTCGGGTGCCAAGGAA4459830.5572463086335112RNA PCR Primer, Index 1 (100% over 23bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG4348110.5432871313553334No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGGAATTCTCGGGTGCCA4086400.5105870213887033No Hit
TGTCAAAGTGAAGAAATTCAATGAAGCTGGAATTCTCGGGTGCCAAGGAA3776980.4719256480140721RNA PCR Primer, Index 1 (100% over 23bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA3696940.4619248196096204No Hit
CCCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT3604020.45031466249640617RNA PCR Primer, Index 1 (100% over 30bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA3330180.41609893473185No Hit
AGAATTCACCAAGCGTTGGATTGTTGGAATTCTCGGGTGCCAAGGAACTC3303550.4127715726577551RNA PCR Primer, Index 1 (100% over 26bp)
CCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC3046150.380609988058731RNA PCR Primer, Index 1 (100% over 31bp)
GAATTCACCAAGCGTTGGATTGTTCACCCACTTGGAATTCTCGGGTGCCA2980080.372354681553457No Hit
AGAATTCACCAAGCGTTGGATTGTTTGGAATTCTCGGGTGCCAAGGAACT2910140.36361582674826765RNA PCR Primer, Index 1 (100% over 25bp)
AGAATTCACCAAGCGTTGGATTGTTCACCCATGGAATTCTCGGGTGCCAA2824630.3529315334341163No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA2604620.3254417501099854No Hit
CGGCGGCGTCCGGTGAGCTCTCGCTGGCCTGGAATTCTCGGGTGCCAAGG2532950.3164867354704669Illumina Small RNA Adapter 2 (100% over 21bp)
GAATTCACCAAGCGTTGGATTGTTCACCCTGGAATTCTCGGGTGCCAAGG2515380.3142914011992748Illumina Small RNA Adapter 2 (100% over 21bp)
GTTGGATTGTTCACCCACTAATAGGGTGGAATTCTCGGGTGCCAAGGAAC2414630.3017029021769294RNA PCR Primer, Index 1 (100% over 24bp)
AGCAGAATTCACCAAGCGTTGGATTGTTTGGAATTCTCGGGTGCCAAGGA2402580.3001972802094926RNA PCR Primer, Index 1 (100% over 22bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCA2399980.2998724157185934No Hit
GCAGAATTCACCAAGCGTTGGATTGTTCTGGAATTCTCGGGTGCCAAGGA2326040.29063376938894364RNA PCR Primer, Index 1 (100% over 22bp)
GGCGGCGTCCGGTGAGCTCTCGCTGGCTGGAATTCTCGGGTGCCAAGGAA2297320.2870452662433182RNA PCR Primer, Index 1 (100% over 23bp)
TACTTGGATAACTGTGGTAATTCTAGAGCTGGAATTCTCGGGTGCCAAGG2286910.28574455880091015Illumina Small RNA Adapter 2 (100% over 21bp)
AGAATTCACCAAGCGTTGGATTGTTCTGGAATTCTCGGGTGCCAAGGAAC2237410.2795596386857132RNA PCR Primer, Index 1 (100% over 24bp)
AGCAGAATTCACCAAGCGTTGGATTGTTGGAATTCTCGGGTGCCAAGGAA2201490.2750715107960592RNA PCR Primer, Index 1 (100% over 23bp)
CAGAATTCACCAAGCGTTGGATTGTTCTGGAATTCTCGGGTGCCAAGGAA2140610.26746468379377253RNA PCR Primer, Index 1 (100% over 23bp)
GAATTCACCAAGCGTTGGATTGTTCACCTGGAATTCTCGGGTGCCAAGGA2113030.2640186212326184RNA PCR Primer, Index 1 (100% over 22bp)
AGCCCGTGGACGGTGTGAGGCCGGTTGGAATTCTCGGGTGCCAAGGAACT2094740.26173332448702336RNA PCR Primer, Index 1 (100% over 25bp)
TGTCAAAGTGAAGAAATTCAATGATGGAATTCTCGGGTGCCAAGGAACTC2072730.2589832216236802RNA PCR Primer, Index 1 (100% over 26bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAG2057330.25705902425450783No Hit
TGTCGGCTCTTCCTATCATTGTGATGGAATTCTCGGGTGCCAAGGAACTC2038290.25468001659807654RNA PCR Primer, Index 1 (100% over 26bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGC2014210.25167127162082514RNA PCR Primer, Index 1 (100% over 50bp)
AATGTCAAAGTGAAGAAATTCAATGAAGCTGGAATTCTCGGGTGCCAAGG1965190.24554632648856345Illumina Small RNA Adapter 2 (100% over 21bp)
CTACTTGGATAACTGTGGTAATTCTAGAGCTGGAATTCTCGGGTGCCAAG1958120.24466294496907978No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC1957220.24455049187607616No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA1950960.24376831814029573No Hit
ACCCCCCTCGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATC1904060.237908262515998RNA PCR Primer, Index 1 (100% over 36bp)
TACTTGGATAACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAG1833140.22904695878731585No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG1793590.22410527008921402No Hit
CTGCCAGTAGCATATGCTTGTCTCTGGAATTCTCGGGTGCCAAGGAACTC1692100.21142430963484357RNA PCR Primer, Index 1 (100% over 26bp)
AGAATTCACCAAGCGTTGGATTGTTCACCCACTGGAATTCTCGGGTGCCA1678830.2097662512524463No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTATGGAATTCTCGGGTGCCAA1630310.20370378006074216No Hit
AATCAGTTATGGTTCCTTTGGTCGCTCGCTGGAATTCTCGGGTGCCAAGG1600060.1999241066570107Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCTGGAATTCTCGGGTGCCAAGGAACTCC1584540.1979849155421045RNA PCR Primer, Index 1 (100% over 27bp)
ATGTCAAAGTGAAGAAATTCAATGAAGCGTGGAATTCTCGGGTGCCAAGG1569490.1961044499313224Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAA1548690.19350553400412854No Hit
CTACTTGGATAACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAA1543650.19287579668330848No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAATTCTCGGGTGCCAA1485250.18557884042618722No Hit
GCGGCGTCCGGTGAGCTCTCGCTGGCCTGGAATTCTCGGGTGCCAAGGAA1435080.17931020523064317RNA PCR Primer, Index 1 (100% over 23bp)
GAATGTCAAAGTGAAGAAATTCAATGATGGAATTCTCGGGTGCCAAGGAA1414760.17677126428638454RNA PCR Primer, Index 1 (100% over 23bp)
TTGGATAACTGTGGTAATTCTAGAGCTGGAATTCTCGGGTGCCAAGGAAC1388080.17343765481823395RNA PCR Primer, Index 1 (100% over 24bp)
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA1357720.1696442371475798No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA1325760.16565090286714154No Hit
CTTTCGATGGTAGTCGCCGTGCCTACCATTGGAATTCTCGGGTGCCAAGG1303290.16284332397848547Illumina Small RNA Adapter 2 (100% over 21bp)
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTGGAATTCTCGGGTGCCA1286780.16078043446127535No Hit
AATTCACCAAGCGTTGGATTGTTCACCCTGGAATTCTCGGGTGCCAAGGA1257980.15718193548516074RNA PCR Primer, Index 1 (100% over 22bp)
CGGCGTCCGGTGAGCTCTCGCTGGCCTGGAATTCTCGGGTGCCAAGGAAC1247130.15582625097506203RNA PCR Primer, Index 1 (100% over 24bp)
AGACGATCAGATACCGTCGTAGTTCCGACCTGGAATTCTCGGGTGCCAAG1213980.1516842287160968No Hit
TGACTCTCTTAAGGTAGCCAAATGCCTGGAATTCTCGGGTGCCAAGGAAC1206530.15075336700178937RNA PCR Primer, Index 1 (100% over 24bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC1197240.1495926011862302No Hit
TTTCGATGGTAGTCGCCGTGCCTACCATGGAATTCTCGGGTGCCAAGGAA1167070.14582292361298793RNA PCR Primer, Index 1 (100% over 23bp)
TACCTGGTTGATCCTGCCAGTAGCATTGGAATTCTCGGGTGCCAAGGAAC1166540.14575670123599693RNA PCR Primer, Index 1 (100% over 24bp)
GTTGGATTGTTCACCCACTAATAGGGATGGAATTCTCGGGTGCCAAGGAA1166400.14573920853264083RNA PCR Primer, Index 1 (100% over 23bp)
TACTTGGATAACTGTGGTAATTCTAGAGCTAATGGAATTCTCGGGTGCCA1165660.14564674710061565No Hit
CTACTTGGATAACTGTGGTAATTCTAGAGCTATGGAATTCTCGGGTGCCA1156630.1445184677341464No Hit
AATCAGTTATGGTTCCTTTGGTCGCTCGCTCTGGAATTCTCGGGTGCCAA1141430.14261925994119704No Hit
CGGCGGCGGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCA1141330.1426067651530855RNA PCR Primer, Index 1 (100% over 37bp)
CGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGGAATTCTCGGGTGCCAAG1124500.14050389231391855No Hit
CCTACTTGGATAACTGTGGTAATTCTAGAGCTGGAATTCTCGGGTGCCAA1121160.14008656639099415No Hit
AATTCACCAAGCGTTGGATTGTTCACCCACTTGGAATTCTCGGGTGCCAA1106680.13827732107244767No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCA1101400.13761759626015999No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTGGAATTCTCGGGTGCCAAGGA1090310.13623192425859365RNA PCR Primer, Index 1 (100% over 22bp)
AATGTCAAAGTGAAGAAATTCAATGATGGAATTCTCGGGTGCCAAGGAAC1087270.13585208270000376RNA PCR Primer, Index 1 (100% over 24bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCTGGAATTCTCGGGTGCCAA1086520.13575837178916744No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCGCTGGAATTCTCGGGTGCCAAG1081290.13510489437093554No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCGCTTGGAATTCTCGGGTGCCAA1057410.13212113896990718No Hit
AATCAGTTATGGTTCCTTTGGTCGCTCGCTTGGAATTCTCGGGTGCCAAG1056080.1319549582880241No Hit
AATCAGTTATGGTTCCTTTGGTCGCTGGAATTCTCGGGTGCCAAGGAACT1050960.13131522513671484RNA PCR Primer, Index 1 (100% over 25bp)
AATCAGTTATGGTTCCTTTGGTCGCTCTGGAATTCTCGGGTGCCAAGGAA1050380.1312427553656681RNA PCR Primer, Index 1 (100% over 23bp)
TTTCGATGGTAGTCGCCGTGCCTACCATTGGAATTCTCGGGTGCCAAGGA1035950.12943975744117733RNA PCR Primer, Index 1 (100% over 22bp)
GCCGGATCCGTAACTTCGGGATAAGGATTTGGAATTCTCGGGTGCCAAGG1034390.1292448387466378Illumina Small RNA Adapter 2 (100% over 21bp)
TTGATCCTTCGATGTCGGCTCTTCCTTGGAATTCTCGGGTGCCAAGGAAC1030660.1287787831500785RNA PCR Primer, Index 1 (100% over 24bp)
CGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTGGAATTCTCGGGT1027200.12834646348142031No Hit
AGTCTGGTGCCAGCAGCCGCGGTATGGAATTCTCGGGTGCCAAGGAACTC1008660.12602992976554656RNA PCR Primer, Index 1 (100% over 26bp)
TGATCCTTCGATGTCGGCTCTTCCTTGGAATTCTCGGGTGCCAAGGAACT975710.12191289708280433RNA PCR Primer, Index 1 (100% over 25bp)
TGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTTGGAATTCTCGGGTGCCA971470.12138311806687635No Hit
AGGTAGCCAAATGCCTCGTCATCTGGAATTCTCGGGTGCCAAGGAACTCC966130.12071589638172177RNA PCR Primer, Index 1 (100% over 27bp)
ATGACTCTCTTAAGGTAGCCAAATGCCTGGAATTCTCGGGTGCCAAGGAA953390.11912406037631552RNA PCR Primer, Index 1 (100% over 23bp)
CGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTGGAATTCTCGGG952400.11900036197401158No Hit
CTTGGATAACTGTGGTAATTCTAGAGCTGGAATTCTCGGGTGCCAAGGAA913030.11408116389451049RNA PCR Primer, Index 1 (100% over 23bp)
AGAACGAAAGTCGGAGGTTCGAAGACGATCTGGAATTCTCGGGTGCCAAG911900.11393997278885046No Hit
AACGCAGCTAGCTGCGAGAATTAATGTGAATTGCTGGAATTCTCGGGTGC892060.11146100682752706No Hit
CCGGCGGCGTCCGGTGAGCTCTCGCTGGCCTGGAATTCTCGGGTGCCAAG880990.11007783378358302No Hit
AATTCACCAAGCGTTGGATTGTTCACCTGGAATTCTCGGGTGCCAAGGAA873150.10909824239564073RNA PCR Primer, Index 1 (100% over 23bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGTGGAATTCTCGGGTGCCA855140.10684793105675795No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGGAATTCTCGGGTGCCAAGG854330.10674672327305472Illumina Small RNA Adapter 2 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA853790.10667925141725257No Hit
AAGCAGAATTCACCAAGCGTTGGATTGTTCTGGAATTCTCGGGTGCCAAG851170.10635188796873105No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAGG844740.10554847309316101Illumina Small RNA Adapter 2 (100% over 21bp)
GTTGGATTGTTCACCCACTAATAGGGAACTGGAATTCTCGGGTGCCAAGG834660.10428899845152091Illumina Small RNA Adapter 2 (100% over 21bp)
GACTCTCTTAAGGTAGCCAAATGCCTGGAATTCTCGGGTGCCAAGGAACT825620.1031594696062405RNA PCR Primer, Index 1 (100% over 25bp)
TTGGATAACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAGGAA821430.10263593798436826RNA PCR Primer, Index 1 (100% over 23bp)
AGCCGGATCCGTAACTTCGGGATAAGGATTTGGAATTCTCGGGTGCCAAG803190.10035688863282903No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)