FastQCFastQC Report
Thu 28 Sept 2023
SRR6107150.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6107150.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31209262
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCGGATAGCTCAGTCGGTAGAGCATCAGATGGAATTCTCGGGTGCCAA9663983.0965102603195165No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATGGAATTCTCGGGTGCCAA7114892.279736701239523No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCTGGAATTCTCGGGTGCCAAG5140381.6470687451693027No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGGAATTCTCGGGTGCCA4517691.4475478465335065No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCTGGAATTCTCGGGTGCCAAGG4145111.3281666192555275Illumina Small RNA Adapter 2 (100% over 21bp)
ATGTCAAAGTGAAGAAATTCAATGAAGCTGGAATTCTCGGGTGCCAAGGA3479731.1149670889366112RNA PCR Primer, Index 1 (100% over 22bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG3276981.0500023999285852No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA2872730.9204735440395868No Hit
CCCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT2589390.8296863924561881RNA PCR Primer, Index 1 (100% over 30bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA2470280.7915214400135447No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA2375210.7610593291183879No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCA2371640.7599154379235241No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGC2294520.7352048247728511RNA PCR Primer, Index 2 (100% over 50bp)
AGAATTCACCAAGCGTTGGATTGTTGGAATTCTCGGGTGCCAAGGAACTC1972620.6320623666141161RNA PCR Primer, Index 1 (100% over 26bp)
ATGTCAAAGTGAAGAAATTCAATGATGGAATTCTCGGGTGCCAAGGAACT1591990.5101017768379144RNA PCR Primer, Index 1 (100% over 25bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG1473650.47218354602553564No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA1462840.4687198306707797No Hit
GAATTCACCAAGCGTTGGATTGTTCACCCTGGAATTCTCGGGTGCCAAGG1443850.4626350985165878Illumina Small RNA Adapter 2 (100% over 21bp)
CCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1241080.3976640011545291RNA PCR Primer, Index 1 (100% over 31bp)
GAATTCACCAAGCGTTGGATTGTTCACCTGGAATTCTCGGGTGCCAAGGA1102590.35328935365405306RNA PCR Primer, Index 1 (100% over 22bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA1100000.3524594718067989No Hit
AGCAGAATTCACCAAGCGTTGGATTGTTCTGGAATTCTCGGGTGCCAAGG1078120.34544873249486Illumina Small RNA Adapter 2 (100% over 21bp)
AGAATTCACCAAGCGTTGGATTGTTCACCCATGGAATTCTCGGGTGCCAA1071050.34318337934424725No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCC1068420.3423406807889273No Hit
AGAATTCACCAAGCGTTGGATTGTTTGGAATTCTCGGGTGCCAAGGAACT1019770.32675235960401755RNA PCR Primer, Index 1 (100% over 25bp)
AGAATTCACCAAGCGTTGGATTGTTCACCCACTTGGAATTCTCGGGTGCC874730.2802789761577829No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA868240.27819946527412276No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCTGGAATTCTCGGGTGCCAAGGAA844980.2707465495339172RNA PCR Primer, Index 1 (100% over 23bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCTGGAATTCTCGGGTGCCAA792180.25382849488719084No Hit
ATGTCAAAGTGAAGACATTCAATGAAGCTGGAATTCTCGGGTGCCAAGGA764240.24487602430329816RNA PCR Primer, Index 1 (100% over 22bp)
GAATTCACCAAGCGTTGGATTGTTCACCCACTTGGAATTCTCGGGTGCCA760010.2435206574253502No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGATGGAATTCTCGGGTGCC723170.2317164692968389No Hit
AGAATTCACCAAGCGTTGGATTGTTCTGGAATTCTCGGGTGCCAAGGAAC678280.21733291867010504RNA PCR Primer, Index 1 (100% over 24bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG658020.2108412560348271Illumina Small RNA Adapter 2 (100% over 21bp)
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA650770.20851822769791867No Hit
AGCAGAATTCACCAAGCGTTGGATTGTTGGAATTCTCGGGTGCCAAGGAA648140.20767552914259876RNA PCR Primer, Index 1 (100% over 23bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC630670.20207783189490353No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGAACTGGAATTCTCGGGTG625360.20037641389918157No Hit
ACCCCCCTCGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGA613270.19650256388632323RNA PCR Primer, Index 2 (100% over 36bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG611200.19583929924392318No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA598700.19183407797339136No Hit
CCCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCACGGAACTCCAGT586430.18790255277423734RNA PCR Primer, Index 1 (96% over 30bp)
ATGTCAAAGTGAAGAACTTCAATGAAGCTGGAATTCTCGGGTGCCAAGGA556010.17815544629027114RNA PCR Primer, Index 1 (100% over 22bp)
TATGACTCTCTTAAGGTAGCCAAATGCCTGGAATTCTCGGGTGCCAAGGA525760.16846281081558417RNA PCR Primer, Index 1 (100% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAA518150.16602443210608442No Hit
AGCAGAGTGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAAG514230.16476839471564564No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGTGGAATTCTCGGGTGCCAAG506710.1623588535992937No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA492730.15787941413033094No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTTGGAATTCTCGGGTGCCAAG478490.1533166660589411No Hit
TCCCTGGTGGTCTAGTGGCTAGGATTCGGCGCTGGAATTCTCGGGTGCCA478430.15329744099684253No Hit
AGCAGAATTCACCAAGCGTTGGATTGTTTGGAATTCTCGGGTGCCAAGGA474930.15217597904109362RNA PCR Primer, Index 1 (100% over 22bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAG467510.14979847969490595No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGAATGGAATTCTCGGGTGC461200.1477766439975415No Hit
GCGGCGTCCGGTGAGCTCTCGCTGGCCTGGAATTCTCGGGTGCCAAGGAA452810.14508833948076055RNA PCR Primer, Index 1 (100% over 23bp)
CGGCGGCGTCCGGTGAGCTCTCGCTGGCCTGGAATTCTCGGGTGCCAAGG444430.142403239140996Illumina Small RNA Adapter 2 (100% over 21bp)
GTTGGATTGTTCACCCACTAATAGGGTGGAATTCTCGGGTGCCAAGGAAC429940.13776038664419554RNA PCR Primer, Index 1 (100% over 24bp)
AGAATTCACCAAGCGTTGGATTGTTGGAATTCTCGGGTGCCACGGAACTC421400.13502401947216824RNA PCR Primer, Index 1 (96% over 26bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTGGAATTCTCGGGTGCCA419940.13455620962777012No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCACTGGAATTCTCGGGTGCCA415640.13317841351070717No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGATGGAATTCTCGGGTGCC414550.1328291582159168No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGTGGAATTCTCGGGTGCCAAG409140.13109569845003063No Hit
CCCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCAAGGACCTCCAGT407750.1306503178447475RNA PCR Primer, Index 1 (96% over 30bp)
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCCCTGGAATTCTCGGGTGCC407380.13053176329513974No Hit
ATGACTCTCTTAAGGTAGCCAAATGCCTGGAATTCTCGGGTGCCAAGGAA405650.12997744067129816RNA PCR Primer, Index 1 (100% over 23bp)
AATTCACCAAGCGTTGGATTGTTCACCCTGGAATTCTCGGGTGCCAAGGA396690.12710649806458094RNA PCR Primer, Index 1 (100% over 22bp)
CAGAATTCACCAAGCGTTGGATTGTTCTGGAATTCTCGGGTGCCAAGGAA390610.1251583584385943RNA PCR Primer, Index 1 (100% over 23bp)
AGGGGCCGGCGGCGGCCGCCGCGGGGCTGGAATTCTCGGGTGCCAAGGAA384750.12328071070696897RNA PCR Primer, Index 1 (100% over 23bp)
CCCGCGAGGGGGGCCCGGGCTGGACTTCTCGGGTGCCAAGGAACTCCAGT380250.12183883104957753RNA PCR Primer, Index 1 (96% over 30bp)
ATGGTTCCTTTGGTCGCTCGCTCTGGAATTCTCGGGTGCCAAGGAACTCC377010.12080067769625567RNA PCR Primer, Index 1 (100% over 27bp)
TGTCAAAGTGAAGAAATTCAATGAAGCTGGAATTCTCGGGTGCCAAGGAA368950.11821811102101679RNA PCR Primer, Index 1 (100% over 23bp)
AGCAGAGTCGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA361940.11597198293250253No Hit
AATTCACCAAGCGTTGGATTGTTCACCTGGAATTCTCGGGTGCCAAGGAA358760.11495305464127924RNA PCR Primer, Index 1 (100% over 23bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAACTGGAATTCTCGGGTG358400.11483770426868793No Hit
TGTCGGCTCTTCCTATCATTGTGAAGCTGGAATTCTCGGGTGCCAAGGAA355750.1139885973593352RNA PCR Primer, Index 1 (100% over 23bp)
CTCCCCCGCTCCCCGTCCTCCCCCTGGAATTCTCGGGTGCCAAGGAACTC346160.1109157916005832RNA PCR Primer, Index 1 (100% over 26bp)
CCCCCGGGGAGCCCGGCGGGCGCCGGCGCGCCTGGAATTCTCGGGTGCCA342400.10971102104240721No Hit
GAATTCACCCAGCGTTGGATTGTTCACCCTGGAATTCTCGGGTGCCAAGG327510.10494000146494974Illumina Small RNA Adapter 2 (100% over 21bp)
TGACTCTCTTAAGGTAGCCAAATGCCTGGAATTCTCGGGTGCCAAGGAAC325810.1043952913721574RNA PCR Primer, Index 1 (100% over 24bp)
CCCGCGAGGGGGGCCCGGGCATGGAATTCTCGGGTGCCAAGGAACTCCAG324200.10387941887251291RNA PCR Primer, Index 1 (100% over 29bp)
GAATTCACCAAGCGTTGGATTGTTCACCCATGGAATTCTCGGGTGCCAAG317690.10179349963481996No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)