FastQCFastQC Report
Thu 28 Sept 2023
SRR6107151.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6107151.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81276178
Total Bases4.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGC1090820513.421158903412017RNA PCR Primer, Index 1 (100% over 50bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGATGGAATTCTCGGGTGCCAA28734783.5354492185889943No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATGGAATTCTCGGGTGCCAA16270312.001854713197759No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGATGGAATTCTCGGGTGCC11644521.4327100863428888No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG7601520.9352703568319859No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA5072690.6241299880021425No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGATGGAATTCTCGGGTGCC4579280.5634221628876299No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGATGGAATTCTCGGGTGC4198900.5166212417124241No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGTGGAATTCTCGGGTGCCAAG3509560.4318067220139215No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGTGGAATTCTCGGGTGCCAAG3317340.4081564957446695No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGATGGAATTCTCGGGTGCCA3243900.39912063778392726No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG2937090.36137156941607174No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATGGAATTCTCGGGTGCCAAGGAAC2821830.3471902923387957RNA PCR Primer, Index 1 (100% over 24bp)
AGAATTCACCAAGCGTTGGATTGTTCACCTGGAATTCTCGGGTGCCAAGG2567250.31586746118893533Illumina Small RNA Adapter 2 (100% over 21bp)
ATGTCAAAGTGAAGAAATTCAATGAAGCTGGAATTCTCGGGTGCCAAGGA2473110.3042847314990624RNA PCR Primer, Index 1 (100% over 22bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCC2441140.30035122960629373No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCTGGAATTCTCGGGTGCCAAG2396170.29481824305271837No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAA2305330.2836415363921271No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGTGGAATTCTCGGGTGCCA2112830.259956859684027No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG1558140.19170930995303445Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAG1521690.18722460103869548No Hit
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA1515330.1864420839277162No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGATGGAATTCTCGGGTGC1360440.16738483938061163No Hit
GGGGATGTAGCTCAGTGGTAGAGCATGCTTGGAATTCTCGGGTGCCAAGG1292110.15897770192884808Illumina Small RNA Adapter 2 (100% over 21bp)
GAATTCACCAAGCGTTGGATTGTTCACCCTGGAATTCTCGGGTGCCAAGG1250400.15384581691329038Illumina Small RNA Adapter 2 (100% over 21bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGATGGAATTCTCGGGTGCC1194850.14701109592038147No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGAATGGAATTCTCGGGTGC1141860.14049135036836993No Hit
AGCAGAGTGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAAG1128600.13885987601434702No Hit
CGCGACCTCAGATCAGACGTGGCTGGAATTCTCGGGTGCCAAGGAACTCC1121910.13803675660043954RNA PCR Primer, Index 1 (100% over 27bp)
AAGCATAGAGACGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT1057220.1300774748537019RNA PCR Primer, Index 1 (100% over 35bp)
ATGTCAAAGTGAAGAAATTCAATGATGGAATTCTCGGGTGCCAAGGAACT1052090.12944629359909124RNA PCR Primer, Index 1 (100% over 25bp)
GGCGGCGTCCGGTGAGCTCTCGCTGGCTGGAATTCTCGGGTGCCAAGGAA1036790.12756382319060328RNA PCR Primer, Index 1 (100% over 23bp)
CCCCCGGGGAGCCCGGCGGGCGCCGGCTGGAATTCTCGGGTGCCAAGGAA1031540.12691787746220057RNA PCR Primer, Index 1 (100% over 23bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGGAATTCTCGGGTGCCA1029840.12670871408347967No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA1019940.1254906449956345No Hit
GGGGATGTAGCTCAGTGGTAGAGCATGCTGGAATTCTCGGGTGCCAAGGA1004350.1235724937754824RNA PCR Primer, Index 1 (100% over 22bp)
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCCCTGGAATTCTCGGGTGCC990420.12185858444278715No Hit
CGCGGGAGGCCCGGGTTCGTTTCCCGGCCAATGTGGAATTCTCGGGTGCC977480.12026648201887644No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCTGGAATTCTCGGGTGCCAA966080.1188638570086305No Hit
GAATTCACCAAGCGTTGGATTGTTCACCTGGAATTCTCGGGTGCCAAGGA942710.11598847573762634RNA PCR Primer, Index 1 (100% over 22bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGATGGAATTCTCGGGTGCCAA935460.11509645544602258No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGTGGAATTCTCGGGTGCCAAGG914630.11253358887028374Illumina Small RNA Adapter 2 (100% over 21bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA901440.11091072712597287No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGATGGACTTCTCGGGTGC897050.1103705934597466No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGATGGAATTCTCGGGTGCCAA881480.10845490298522649No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGTGGAATTCTCGGGTGCCA875690.10774251712475948No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGGAATTCTCGGGT872840.107391860872198No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTTGGAATTCTCGGGTGCCAAG867400.10672253806029118No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGTGGAATTCTCGGGTGCCAAG856860.10542572511222169No Hit
CCCCCGGGGAGCCCGGCGGGCGCCGGCGCGCCTGGAATTCTCGGGTGCCA844770.1039382043776714No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGTGGAATTCTCGGGTGCCAAG835020.10273859088206633No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGCTGGAATTCTCGGGTGC831910.1023559449362887No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGTGGAATTCTCGGGTGCC825820.10160664789134154No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)