FastQCFastQC Report
Wed 9 Aug 2023
SRR6123777_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6123777_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1226762
Total Bases368 Mbp
Sequences flagged as poor quality0
Sequence length300
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCTCTCTGGTTAGACCAGATCTGAGCCTGGGAGCTCTCTGGCTAACTA32739326.687572650603787No Hit
GCGAATCGTTCTAGCTCCCTGCTTGCCCATACTATATGTTTTAGTTTATA14441211.771802517521737No Hit
GCGAATCGTTCTAGCTCCCTGCTTGCTTTATTGAGGCTTAAGCAGTGGGT793036.464416080706771No Hit
CCGAAACCTTTATCAGCGTTAAACCATTTTACTAAACCAGTCATTTTATT743836.063360293194605No Hit
GACGTCGGTTTGAAGAACAGACGATATACGAAGTAGTTTACTAAAGCAGT544964.442263454525001No Hit
GCGAATCGTTCTAGCTCCCTGCTTGCTTAAGCAGTGGGTTCCCTAGTTAG143031.1659148229240879No Hit
CCGAAACCTTTCTCAGCGTTAAACCATTTTACTAAACCAGTCATTTTATT121190.9878851806625897No Hit
GCGAATCGTTCTAGCTCCCTGCTTGCGGCTTAAGCAGTGGGTTCCCTAGT105060.8564008340656133No Hit
GCGAATCGTTCTAGCTCCCTGCTTGCACTACTTTGAGCACTCAAGGCAAG99560.8115673618843753No Hit
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTAGGCTATCTCGTA74170.6045997512149871Illumina Paired End Sequencing Primer 2 (100% over 36bp)
GCGAATCGTTCTAGCTCCCTGCTTGCAGTGGGTTCCCTAGTTAGCCAGAG71630.5838948386076517No Hit
GGTCTCTCTGGTTAGACCAGATCTGAGCCTGGGTGCTCTCTGGCTAACTA65130.5309098260298248No Hit
CCGAAACCTTTATCAGCGTTAAACCATTTTACTAAACCAGTCATTTAATT58540.477191174816305No Hit
CCGAAACCTTTATCAGCGTTAAACCATTTTACTAAACCAGTCATTTTAAT58270.474990258909226No Hit
CCGAAACCTTTATCAGCGTTAAACCATTTTACTAAACCAGTCATTTTATA56690.4621108250826159No Hit
GCCGAAACCTTTATCAGCGTTAAACCATTTTACTAAACCAGTCATTTTAT56540.4608880940231276No Hit
GACGTCGGTTTGAAGAACAGACGATATATGAAGTAGTTTACTAAAGCAGT54300.4426286435347687No Hit
GCGAATCGTTCTAGCTCCCTGCTTGCGGATAAGCACTACTTTGAGCACTC54110.4410798508594169No Hit
GACGTCGGTTTGAAGAACAGACGATTTACGAAGTAGTTTACTAAAGCAGT52500.427955870820909No Hit
GACGTCGGTTTGAAGAACAGACGATATTCGAAGTAGTTTACTAAAGCAGT51220.4175218991132755No Hit
GACGTCGGTTTGAAGAACAGACGATATAGGAAGTAGTTTACTAAAGCAGT49840.40627277336598294No Hit
GCGAATCGTTCTAGCTCCCTGCTTGCGCACTCAAGGCAAGCTTTATTGAG46290.37733480495809296No Hit
GCGAATCGTTCTAGCTCCCTGCTTGCAAGTCCCTGTTCGGGCGCCACTGC43160.35182048351677014No Hit
GACGTCGGTTTGAAGAACAGACGATATACGATGTAGTTTACTAAAGCAGT43100.35133139109297484No Hit
GCGAATCGTTCTAGCTCCCTGCTTGCAAGCTTTATTGAGGCTTAAGCAGT38770.31603522117574556No Hit
GACGTCGGTTTGAAGAACAGACGATATACGTAGTAGTTTACTAAAGCAGT35430.2888090762511392No Hit
GCGAATCGTTCTAGCTCCCTGCTTGCTTTCAAGTCCCTGTTCGGGCGCCA34550.28163572070214105No Hit
GTCTCTCTGGTTAGACCAGATCTGAGCCTGGGAGCTCTCTGGCTAACTAG29340.23916619523591376No Hit
GACGTCGGTTTGAAGAACAGACGATATAAGAAGTAGTTTACTAAAGCAGT28910.2356610328653806No Hit
GACGTCGGTTTGAAGAACAGACGGTATACGAAGTAGTTTACTAAAGCAGT28850.23517194044158524No Hit
GACGTCGGTTTGAAGAACAGACGATATACGAAGTTGTTTACTAAAGCAGT28770.23451981720985815No Hit
GCGAATCGTTCTAGCTCCCTGCTTGCTTTATTGAGGCTAAGCAGTGGGTT28430.23174829347501796No Hit
CGAATCGTTCTAGCTCCCTGCTTGCCCATACTATATGTTTTAGTTTATAT27570.22473796873395163No Hit
GGTCTCTCTGGTTAGACCAGATCTGAGCCTGGGCGCTCTCTGGCTAACTA25600.20867943415267182No Hit
GCGAATCGTTCTAGCTCCCTGCTTGCTAGAGATTTTCCACACTGACTAAA25540.20819034172887652No Hit
AATCGTTCTAGCTCCCTGCTTGCCCATACTATATGTTTTAGTTTATATTG25400.2070491260733541No Hit
GACGTCGGTTTGAAGAACAGACGATATACGGAGTAGTTTACTAAAGCAGT25210.20550033339800222No Hit
GCGAATCGTTCTAGCTCCCTGCTTGCTTTATTGAGGCATAAGCAGTGGGT23330.19017543745241539No Hit
TAGCTCCCTGCTTGCCCATACTATATGTTTTAGTTTATATTGTTTCTTTC21080.1718344715600907No Hit
GAAACCTTTATCAGCGTTAAACCATTTTACTAAACCAGTCATTTTATTTG20770.1693074940371482No Hit
GACGTCGGTTTGAAGAACAGACGTTATACGAAGTAGTTTACTAAAGCAGT20670.16849233999748933No Hit
CTCTCTGGTTAGACCAGATCTGAGCCTGGGAGCTCTCTGGCTAACTAGGG20090.16376444656746786No Hit
CGAATCGTTCTAGCTCCCTGCTTGCTTTATTGAGGCTTAAGCAGTGGGTT19410.1582213990977875No Hit
GCGAATCGTTCTAGCTCCCTGCTTGCCGTGCGCGCTTCAGCAAGCCGAGT19370.15789533748192397No Hit
GTTCTAGCTCCCTGCTTGCCCATACTATATGTTTTAGTTTATATTGTTTC19300.15732472965416275No Hit
ATCGTTCTAGCTCCCTGCTTGCCCATACTATATGTTTTAGTTTATATTGT18660.15210774380034595No Hit
CGTTCTAGCTCCCTGCTTGCCCATACTATATGTTTTAGTTTATATTGTTT18460.1504774357210282No Hit
GGTCTCTCTGGTTAGACCAGATCTGAGCCTGGGGCTCTCTGGCTAACTAG18310.1492547046615399No Hit
TTCTAGCTCCCTGCTTGCCCATACTATATGTTTTAGTTTATATTGTTTCT17870.14566802688704084No Hit
GACGTCGGTTTGAAGAACAGACGATATACGAGGTAGTTTACTAAAGCAGT17810.1451789344632455No Hit
CCGAACCCTTTATCAGCGTTAAACCATTTTACTAAACCAGTCATTTTATT17410.14191831830461002No Hit
CTCTGGTTAGACCAGATCTGAGCCTGGGAGCTCTCTGGCTAACTAGGGAA17100.1393913407816675No Hit
AATCGTTCTAGCTCCCTGCTTGCTTTATTGAGGCTTAAGCAGTGGGTTCC15420.12569675291539842No Hit
AGCTCCCTGCTTGCCCATACTATATGTTTTAGTTTATATTGTTTCTTTCC15380.12537069129953488No Hit
GCGAATCGTTCTAGCTCCCTGCTTGCTAAAAGGGTCTGAGGGATCTCTAG15100.12308825998849002No Hit
GGTCTCTTGGTTAGACCAGATCTGAGCCTGGGAGCTCTCTGGCTAACTAG14790.12056128246554752No Hit
CCGAAACCTTTATCAGCGTTAAACCATTTTACTAAACCAGTCAATTTATT14740.12015370544571809No Hit
GCGAATCGTTCTAGCTCCCTGCTTGCCAGAGAGCTCCCAGGCTCAGATCT14740.12015370544571809No Hit
GCGAATCGTTCTAGCTCCCTGCTTGCACTCAAGGCAAGCTTTATTGAGGC14690.11974612842588864No Hit
CCGAAACCTTTATCAGCGTTAAACCATTTTACTAAACCAGTCATATTATT14250.11615945065138959No Hit
CTAGCTCCCTGCTTGCCCATACTATATGTTTTAGTTTATATTGTTTCTTT14150.11534429661173072No Hit
TCTAGCTCCCTGCTTGCCCATACTATATGTTTTAGTTTATATTGTTTCTT13910.1133879269165494No Hit
GGTTCTCTGGTTAGACCAGATCTGAGCCTGGGAGCTCTCTGGCTAACTAG13550.11045337237377748No Hit
GGTTAGACCAGATCTGAGCCTGGGAGCTCTCTGGCTAACTAGGGAACCCA13520.1102088261618798No Hit
GCGAATCGTTCTAGCTCCCTGCTTGCTTTATTGAGGCTTAAGCAGAGGGT13340.10874154889049384No Hit
GCTCCCTGCTTGCCCATACTATATGTTTTAGTTTATATTGTTTCTTTCCC12840.10466577869219947No Hit
GCGAATCGTTCTAGCTCCCTGCTTGCAACAGACGGATAAGCACTACTTTG12600.10270940899701816No Hit
GCGAATCGTTCTAGCTCCCTGCTTGCGCCACTGCTAGAGATTTTCCACAC12560.10238334738115462No Hit
GCGAATCGTTCTAGCTCCCTGCTTGCTTTGAGCACTCAAGGCAAGCTTTA12530.10213880116925694No Hit
GGTCTCTCTGTTAGACCAGATCTGAGCCTGGGAGCTCTCTGGCTAACTAG12510.10197577036132517No Hit
GAATCGTTCTAGCTCCCTGCTTGCCCATACTATATGTTTTAGTTTATATT12300.10026394687804153No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)